The Journal of Engineering Geology (지질공학)

The Korean Society of Engineering Geology (대한지질공학회)
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Characterization of Water Quality and the Aerobic Bacterial Population in Leachate Derived from Animal Carcass Disposal

가축 매몰지 침출수에 대한 수질 특성 및 호기성 미생물 분포에 관한 연구

  • Choi, Nag-Choul (Department of Rural Systems Engineering/Research Institute for Agriculture and Life Sciences, Seoul National University) ;
  • Choi, Eun-Ju (Department of Energy and Resource Engineering, Chosun University) ;
  • Kim, Bong-Ju (Department of Energy and Resource Engineering, Chosun University) ;
  • Park, Jeong-Ann (Department of Rural Systems Engineering/Research Institute for Agriculture and Life Sciences, Seoul National University) ;
  • Kim, Song-Bae (Department of Rural Systems Engineering/Research Institute for Agriculture and Life Sciences, Seoul National University) ;
  • Park, Cheon-Young (Department of Energy and Resource Engineering, Chosun University)
  • 최낙철 (서울대학교 지역시스템공학과) ;
  • 최은주 (조선대학교 에너지자원공학과) ;
  • 김봉주 (조선대학교 에너지자원공학과) ;
  • 박정안 (서울대학교 지역시스템공학과) ;
  • 김성배 (서울대학교 지역시스템공학과) ;
  • 박천영 (조선대학교 에너지자원공학과)
  • Received : 2013.02.14
  • Accepted : 2013.03.21
  • Published : 2013.03.31
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Abstract

Leakage of leachate from animal carcass disposal is a significant issue because disease can easily spread to humans and other livestock. In this study, we analyzed the physicochemical properties of leachate and tested for the presence of aerobic bacteria in leachate using molecular biology methods, for 16 animal carcass disposals in the first stage (after burial for 5 months). Leachate physicochemical analysis revealed higher total coliforms, TOC, $NH^{4+}$, and $NO^{3-}$ concentrations compared with previously published data. In most leachate samples, the concentrations of $NH^{4+}$ and $NO^{3-}$ exceeded the Korean guideline values for drinking water. In 16S rRNA sequence analysis of the distribution of leachate under aerobic conditions, Bacillus pumilus, Lysinibacillus sphaericus, and B. sphaericus were observed with high frequency, whereas no food-poisoning-related bacteria such as B. cereus or Salmonella were detected. The present findings improve our knowledge of the transport of leachate from animal carcass disposal sites through geologic media, and are useful in risk analysis and for subsequent studies.

가축 매몰지내 침출수의 누출은 인간과 다른 가축들에게 쉽게 질병을 확산시킬 수 있는 중요한 문제이다. 본 연구에서는 매몰된지 5개월 이내의 초기 16개 가축매몰지에 대한 침출수의 물리화학적 특성과 분자미생물학적 방법을 이용하여 침출수내 존재하는 호기성 미생물을 분석하였다. 총대장균군, 총유기탄소, 암모니아이온 및 질산성질소가 관련된 참고문헌자료에 비하여 높게 나타났다. 또한 암모니아 이온과 질산성질소는 국내 먹는물 기준치를 초과하여 나타났다. 16S rNA 서열 분석법을 사용하여 호기성 조건에서 침출수의 미생물 분포를 분석한 결과, 높은 빈도의 Bacillus pumilus, Lysinibacillus sphaericus 및 Bacillus sphaericus이 관찰되었지만, Bucillus cereus와 Salmonella와 같은 식중독 미생물은 발견되지 않았다. 본 연구는 가축매몰지로부터 형성되는 침출수에 대한 지질 매체 내에서의 거동특성, 처리 및 위해성 평가에 대한 기초자료로 활용 가능할 것이다.

Keywords

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