The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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The anti-inflammatory effect of Lithospermum Erythrorhizon on lipopolysaccharide - induced inflammatory response in RAW 264.7 cells

LPS로 유도한 RAW 264.7 세포의 염증반응에서 자초(紫草)의 항염증 효과

  • Choi, Sun-Bok (Dept. of Herbology, College of Oriental Medicine, Wonkwang University) ;
  • Bae, Gi-Sang (Hanbang Body-fluid Research Center, Wonkwang University) ;
  • Jo, Il-Joo (Dept. of Herbal Resources, Professional Graduate School of Oriental Medicine Wonkwang University) ;
  • Park, Kyoung-Chel (Dept. of Herbology, College of Oriental Medicine, Wonkwang University) ;
  • Seo, Seung-Hee (Dept. of Herbal Resources, Professional Graduate School of Oriental Medicine Wonkwang University) ;
  • Kim, Dong-Goo (Dept. of Herbal Resources, Professional Graduate School of Oriental Medicine Wonkwang University) ;
  • Shin, Joon-Yeon (Dept. of Herbal Resources, Professional Graduate School of Oriental Medicine Wonkwang University) ;
  • Gwak, Tae-Sin (Dept. of Herbology, College of Oriental Medicine, Wonkwang University) ;
  • Lee, Jung-Hyun (Dept. of Herbology, College of Oriental Medicine, Wonkwang University) ;
  • Lee, Guem-San (Dept. of Herbology, College of Oriental Medicine, Wonkwang University) ;
  • Park, Sung-Joo (Dept. of Herbology, College of Oriental Medicine, Wonkwang University) ;
  • Song, Ho-Joon (Dept. of Herbology, College of Oriental Medicine, Wonkwang University)
  • 최선복 (원광대학교 한의과대학 본초학교실) ;
  • 배기상 (원광대학교 한방체액조절연구센터) ;
  • 조일주 (원광대학교 한약자원전문대학원) ;
  • 박경철 (원광대학교 한의과대학 본초학교실) ;
  • 서승희 (원광대학교 한약자원전문대학원) ;
  • 김동구 (원광대학교 한약자원전문대학원) ;
  • 신준연 (원광대학교 한약자원전문대학원) ;
  • 곽태신 (원광대학교 한의과대학 본초학교실) ;
  • 이정현 (원광대학교 한의과대학 본초학교실) ;
  • 이금산 (원광대학교 한의과대학 본초학교실) ;
  • 박성주 (원광대학교 한의과대학 본초학교실) ;
  • 송호준 (원광대학교 한의과대학 본초학교실)
  • Received : 2013.02.15
  • Accepted : 2013.03.21
  • Published : 2013.03.30
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Abstract

Objective : Lithospermum Erythrorhizon (LE) has been used as an anti-bacterial and anti-inflammatory agent. However, it is unclear that LE aqueous extract could show the anti-inflammatory effects in RAW 264.7cells. The purpose of this study was to investigate the anti-inflammatory effect of aqueous extract from LE on lipopolysaccharide (LPS) - induced inflammatory response. Methods : To measure out the cytotoxicity of LE, we performed the MTT assay. To evaluate the anti-inflammatory effects of LE, we examined the inflammatory mediators such as nitric oxide (NO), prostaglandin E2 ($PGE_2$) and pro-inflammatory cytokines (tumor necrosis factor (TNF)-${\alpha}$, interleukin, (IL)-$1{\beta}$ and (IL)-6) on RAW 264.7 cells. We also examined molecular mechanisms such as mitogen-activated protein kinases (MAPKs) and nuclear factor-B (NF-${\kappa}B$) activation by western blot. Results : Aqueous Extract from LE itself did not have any cytotoxic effect in RAW 264.7 cells. Aqueous extract from LE inhibited LPS-induced productions of inflammatory mediators such as NO, $PGE_2$, and pro-inflammatory cytokines including TNF-${\alpha}$, IL-$1{\beta}$ and IL-6 in RAW 264.7cells. In addition, LE inhibited the phosphorylation of p38 kinases (p38), c-Jun $NH_2$-terminal kinase (JNK), and NF-${\kappa}B$ activation in RAW 264.7 cells. Conclusion : LE down-regulated LPS-induced production of inflammatory mediators through the inhibition of p38, JNK and NF-${\kappa}B$ activation. Taken together, these results could provide the evidence for the anti-inflammatory effects of LE. Therefore, LE may be a novel target in the management of inflammation and help to support a potential strategy for prevention and therapy of inflammatory diseases.

Keywords

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