Korean Journal of Soil Science and Fertilizer (한국토양비료학회지)

Korean Society of Soil Science and Fertilizer (한국토양비료학회)
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Relationship Between Relative Water Content and Ascorbate Redox Enzymes Activity in Lettuce Leaves Subjected to Soil Water Stress

토양 수분 Stress에 따른 상추의 엽중 상대수분 함량과 아스코브산 관련 효소 활성도

  • Kang, Sang-Jae (School of Applied Life Sciences, Kyungpook National University) ;
  • Park, Man (School of Applied Life Sciences, Kyungpook National University)
  • 강상재 (경북대학교 농업생명과학대학 응용생명과학부) ;
  • 박만 (경북대학교 농업생명과학대학 응용생명과학부)
  • Received : 2013.01.25
  • Accepted : 2013.02.12
  • Published : 2013.02.28
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Abstract

The relationship between relative water contents of lettuce leaves and biochemical activities in lettuce was examined in this study to explore an adaptation response of lettuce to water stress from soils. Soil water contents and relative water contents of leaves were positively related to show $R^2$=0.8728. Hydrogen peroxide contents of leaves rapidly increased with reduction of soil water content, whereas soluble protein contents and dry matters rapidly decreased. And chlorophyll a and b contents of leaves decreased with increase in carotenoid content. Furthermore, the activities of ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR), and dehydroascorbate reductase (DHAR) increased dramatically, and mRNA transcript levels of APX, MDHAR and DHAR also increased. Relationship of relative water content of lettuce leaves to hydrogen peroxide, to ascorbate peroxidase activity, to dehydroascorbate reductase activity, and to monodehydroascorbate reductase activity was shown to be positively correlated. It is highly plausible from this study that these enzyme activities could be developed as an indicator of water states in soils.

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References

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