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Effects of Water-soaking and Mechanical and Chemical Scarifications on Seed germination of Hairy Vetch (Vicia villosa Roth)

헤어리베치 종자의 침지처리와 종피 파상 및 황산처리가 발아에 미치는 영향

  • 김민태 (농촌진흥청 국립식량과학원) ;
  • 이용환 (농촌진흥청 국립식량과학원) ;
  • 전원태 (농촌진흥청 국립식량과학원) ;
  • 김숙진 (농촌진흥청 국립식량과학원) ;
  • 윤다해 (농촌진흥청 국립식량과학원) ;
  • 구자환 (농촌진흥청 국립식량과학원) ;
  • 송하나 (농촌진흥청 국립식량과학원) ;
  • 이현복 (농촌진흥청 국립식량과학원) ;
  • 서명철 (농촌진흥청 국립식량과학원) ;
  • 강항원 (농촌진흥청 국립식량과학원)
  • Received : 2012.11.19
  • Accepted : 2013.02.01
  • Published : 2013.02.28

Abstract

Seed coat of hairy vetch is very thick and hard, and difficult to absorb water during germination. It requires much time that cotyledon comes out from seed coat. Therefore this experiment was carried out to increase the germination rate by enhancing water absorption through water-soaking of seed, chemical scarification by sulfuric acid, and mechanical scarification on seed coat. Water-soaking for 5 hours seemed to be highly effective in enhancing germination rate. Although not significant, water-soaking resulted in increase of germination rate by 9%. Effect of soaking temperature was not significant, but early germination rate was increased to be 5.0%, 31.7% and 48.3% at $10^{\circ}C$, $20^{\circ}C$ and $30^{\circ}C$, respectively. Mechanical scarification of seed coat led to a germination rate of 97% whereas intact seed showed that of 65%. Chemical scarification by sulfuric acid for 10min, 20min and 30min resulted in a germination rate of 76.7%, 74.7% and 96.0% respectively. It is clear that scarification increased germination rate.

헤어리베치와 같은 두과 녹비작물은 종피가 딱딱하여 발아율 감소의 원인이 되는 경실률이 환경 및 저장조건에 따라 10~40%나 된다. 따라서 헤어리베치의 종자 발아율을 증진하기 위하여 침수처리, 종피파상 및 황산처리가 발아에 미치는 영향 조사한 결과 다음과 같다. 헤어리베치 종자를 증류수에 1~9시간까지 침지 시킨 후 발아율을 조사한 결과 5시간 침지조건에서 초기발아가 가장 좋았으며 발아율에는 큰 차이는 보이지 않았지만 무침지에 비하여 9% 높은 것으로 나타났다. 온도 조건에 따른 발아율의 차이는 83.0~85.7%로 큰 차이는 없었지만 초기 발아 속도는 $10^{\circ}C$의 5%에 비하여 $20^{\circ}C$ 31.7%, $30^{\circ}C$ 48.3%로 발아율의 증가를 보였다. 종피파상 (칼집처리) 시험 시 무처리 헤어리베치 종자의 발아율은 65%였고 파상처리 종자의 발아율은 97%로 헤어리베치 종피의 파상처리는 종자의 발아율을 상승시키는데 효과가 큰 것으로 나타났다. 헤어리베치 종자를 10분, 20분 동안 황산처리된 종자의 발아율은 각각 76.7%, 74.7%를 보였으며 30분 동안 처리된 종자는 96.0%의 발아율로 가장 높았다.

Keywords

References

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