The Korean Journal of Malacology (한국패류학회지)

The Malacological Society of Korea (한국패류학회)
권호 표지
DOI QR코드

DOI QR Code

Concentration of Heavy Metal and Alteration of Reproductive and Histological Biomarker of Mytilus galloprovincialis in Gamak Bay of the Southern Coast of Korea

한국 남해안 가막만에서 채집된 지중해담치, Mytilus galloprovincialis의 체내 중금속 농도와 생식 및 조직학적 지표 변화

  • Jeon, Mi Ae (Department of Aqualife Medicine, Chonnam National University) ;
  • Kang, Ju Chan (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Lee, Jung Sick (Department of Aqualife Medicine, Chonnam National University)
  • 전미애 (전남대학교 수산생명의학과) ;
  • 강주찬 (부경대학교 수산생명의학과) ;
  • 이정식 (전남대학교 수산생명의학과)
  • Received : 2013.03.18
  • Accepted : 2013.03.26
  • Published : 2013.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study investigated concentration of metal ions, intersexuality and histological alterations of digestive gland in Mytilus galloprovincialis. Samples were collected from two areas of Gamak Bay in May, 2010. The concentration of metal ions showed that Al ($366.5{\pm}249.7$ mg/kg) was highest and Zn ($179.5{\pm}67.8$ mg/kg) was second high concentration. Co ($1.0{\pm}0.2$ mg/kg) was lowest. The intersexuality was 26.4% and males (38.8%) was higher than the females (12.9%). Intersex type was observed four types. Destruction of digestive tubule epithelium was highest among other biomarker in digestive gland. Distribution of basophilic cell and lipofuscin was 6.1% and 1.5%, respectively.

본 연구는 지중해담치, Mytilus galloprovincialis의 체내 중금속 농도, 이성생식세포 발현과 소화선의 조직학적 지표 변화를 이용하여 이들의 안정성을 알아보고자 하였다. 시료는 2010년 5월 한국 남해안의 가막만 2곳에서 채집하였다. 금속이온의 체내 축적 농도는 분석된 10가지 금속 이온 가운데 Al이 가장 높았으며, 두번째로 Zn이 높았다. 그리고 Co가 가장 낮은 농도로 나타났다. 이성생식세포 발현율은 26.4%로 나타났으며, 수컷에서 더 높았다. 이성생식세포 발현 형태는 네 가지 형태가 관찰되었다. 조직학적 지표는 소화선의 조직학적 변화, 호염기성세포와 지방갈색소의 분포비율을 이용하였다. 소화선에서는 소화선세관 상피층의 파괴가 가장 높은 비율로 나타났다. 호염기성세포의 분포 비율은 6.1%로 나타났고, 지방 갈색소 분포 비율은 1.5%였다.

Keywords

References

  1. Andhale, A.V., Bhosale, P.A. and Zambare, S.P. (2011) Histopathological study of nickel induced alterations in the fresh water bivalve, Lammellidens marginalis. Journal of Experimental Sciences, 2: 1-3.
  2. Byrne, P.A. and O'Halloran, J. (1999) Aspects of assaying sediment toxicity in Irish estuarine ecosystems. Marine Pollution Bulletin, 39: 97-105. https://doi.org/10.1016/S0025-326X(99)00121-6
  3. Byrne, P.A. and O'Halloran, J. (2001) The role of bivalve molluscs as tools in estuarine sediment toxity testing: a review. Hydrobiologia, 465: 209-217. https://doi.org/10.1023/A:1014584607501
  4. Cajaraville, M.P., Diez, G., Marigomez, I.A. and Angulo, E. (1990) Responses of basophilic cells of the digestive gland of mussels to petroleum hydrocarbon exposure. Diseases of Aquatic Organisms, 9: 221-228. https://doi.org/10.3354/dao009221
  5. Chesman, B.S. and Langston, W.J. (2006) Intersex in the clam Scrobicularia plana: a sign of endocrine disruption in estuaries? Biology Letters, 2: 420-422. https://doi.org/10.1098/rsbl.2006.0482
  6. Choi, H.G., Park, J.S. and Lee, P.Y. (1992) Study on the heavy metal concentration in mussels and oysters from the Korean coastal waters. Journal of the Korean Fisheries Society, 25: 485-494.
  7. Da Ros, L., Nasci, C., Marigomez, I. and Soto, M. (2000) Biomarkers and trace metals in the digestive gland of indigenous and transplanted mussels, Mytilus galloprovincialis, in Venice Lagoon, Italy. Marine Environmental Research, 50: 417-423. https://doi.org/10.1016/S0141-1136(00)00038-6
  8. Depledge, M.H. and Hopkin, S.P. (1995) Methods to assess effects on brackish, estuarine and near-coastal water organisms. In: Methods to assess the effects of chemicals on ecosystems. (ed. by Linthurst, R.A., Bourdeau, P. and Tardiff, R.G.), Wiley, Chichester, U.K., pp. 125-149.
  9. Dimitriadis, V.K., Domouhtsidou, G.P. and Raftopoulou, E. (2003) Localization of Hg and Pb in the palps, the digestive gland and the gills in Mytilus galloprovincialis (L.) using autometallography and X-ray microanalysis. Environmental Pollution, 125: 345-353. https://doi.org/10.1016/S0269-7491(03)00122-2
  10. FDA (Food and Drug Administration) (2011) Fish and fishery products hazards and controls guidance. 5. FDA & EPA safety levels in regulations and guidance. F.D.A., U.S., pp. 476.
  11. Food Standards (2011) Food standards code. 2. food product standards. Food Standards Australia New Zealand, Australia, pp. 123.
  12. Gibbs, P.E., Pascoe, P.L. and Burt, G.R. (1988) Sex change in the female dog-whelk, Nucella lapillus, induced by tributyltin from antifouling paints. Journal of the Marine Biological Association of the United Kingdom, 68: 715-731. https://doi.org/10.1017/S0025315400028824
  13. Goldberg, E.D. (1975) The mussel watch. A first step in global marine pollution monitoring. Marine Pollution Bulletin, 6: 1-111. https://doi.org/10.1016/0025-326X(75)90076-4
  14. Hebel, D.K., Jones, M.B. and Depledge, M.H. (1997) Responses of crustaceans to contaminant exposure: a holistic approach. Estuarine, Coastal and Shelf Science, 44: 177-184. https://doi.org/10.1006/ecss.1996.0209
  15. Horiguchi, T., Shiraishi, H., Shimizu, M. and Morita, M. (1994) Imposex and organotin compounds in Thais clavigera and T. bronniin Japan. Journal of the Marine Biological Association of the United Kingdom, 74: 651-669. https://doi.org/10.1017/S002531540004772X
  16. Horiguchi, T., Takiguchi, N., Cho, H.S., Kojima, M., Kaya, M., Shiraishi, H., Morita, M., Hirose, H. and Shimizu, M. (2000) Ovo-testis and disturbed reproductive cycle in the giant abalone Haliotis madaka: possible linkage with organotin contamination in a site of population decline. Marine Environmental Research, 50: 223-229. https://doi.org/10.1016/S0141-1136(00)00071-4
  17. Huggett, R.J., Kimerle, R.A., Jr, P.M.M. and Bergman, H.L. (1992) Biomarkers: biochemical, physiological, and histological markers of anthropogenic stress. Lewis Publishers, London, pp. 347.
  18. Husmann, G., Abele, D., Monien, D., Monien, P., Kriews, M. and Philipp, E.E.R. (2012) The influence of sedimentation on metal accumulation and cellular oxidative stress markers in the Antarctic bivalve Laternula elliptica. Estuarine, Coastal and Shelf Science, 111: 48-59. https://doi.org/10.1016/j.ecss.2012.06.003
  19. Iguchi, T. (1998) Environmental endocrine disruptors. Japanese Journal of Clinical Medicine, 56: 2953-2962.
  20. Ju, S.M., Park, J.J. and Lee, J.S. (2009) Induction of intersex and masculinization of the equilateral venus, Gomphina veneriformis (Bivalvia: Veneridae) by zinc. Animal Cells and Systems, 13: 339-344. https://doi.org/10.1080/19768354.2009.9647227
  21. Ju, S.M. and Lee, J.S. (2011) Microanatomical structure of the digestive diverticulum of Mytilus galloprovincialis (Bivalvia: Mytilidae). Korean Journal of Microscopy, 41: 257-263.
  22. Ju, S.M., Lee, J.W., Jin, Y.G., Yu, J. and Lee, J.S. (2006) Effect of zinc bioaccumulation on survival rate, activity, growth and organ structure of the equilateral venus, Gomphina venerifomis (Bivalvia: Veneridae). Journal of Environmental Toxicology, 21: 115-126.
  23. Kahng, S.H., Je, J.G., Oh, J.R., Shim, W.J. and Shim, J.H. (1996) Imposex of Thais clavigera and T. luteostoma (Muricidae) as evidence of organotin pollution in Chinhae bay. The Korean Journal of Malacology, 12: 123-131.
  24. Kim, J.H., Cho, H.S., Yu, J., Choi, Y.S. and Jung, C.S. (2006) A study on the distribution of organochloride compounds in Gwangyang Bay and Gamak Bay. Journal of the Korean Socety for Marine Environmental Engineerning, 11: 40-43.
  25. Krishnakumar, P.K., Asokan, P.K. and Pillai, V.K. (1990) Physiological and cellular responses to copper and mercury in the green mussel Perna viridis (Linnaeus). Aquatic Toxicology, 18: 1630-174.
  26. Lee, J.H., Jeong, H.S., You, Y.Y., Seo, S.J., Jeong, J.Y., Kim, K.B., Cheoi, M.H. and Cho, H.S. (2011) Heavy metal pollution situation in marine organisms of the early stage after the Hebei spirit oil pollution accident. Journal of the Korean Socety for Marine Environmental Engineerning, 11: 108-111.
  27. Lee, J.S. and Park, J.J. (2007) Risk assessment of nonylphenol using the sex ratio, sexual maturation, intersex and lipofuscin accumulation of the equilateral venus, Gomphina veneriformis (Bivalvia: Veneridae). Korean Journal of Fisheries and Aquatic Sciences, 40: 16-23. https://doi.org/10.5657/kfas.2007.40.1.016
  28. Lee, J.S., Lee, Y.G., Kang, S.W., Park, J.S., Lee, D.G., Jeon, M.A. and Ju, S.M. (2010) Intersexuality of Crassostrea gigas and Ruditapes philippinarum in Southern coastal waters of Korea. Journal of Environmental Toxicology, 25: 287-294.
  29. Lee, M.O., Kim, B.K., Park, S.J. and Kim, J.K. (2005) Marine environment of Gamak Bay by field observations and numerical experiments. Journal of the Korean Socety for Marine Environmental Engineerning, 11: 109-114.
  30. Marin, M.G., Boscolo, R., Cella, A., Degetto, S. and Ros, L.D. (2006) Field validation of autometallographical black silver deposit (BSD) extent in three bivalve species from the Lagoon of Venice, Italy (Mytilus galloprovincialis, Tapes philippinarum, Scapharca inaequivalvis) for metal bioavailability assessment. Science of the Total Environment, 371: 156-167. https://doi.org/10.1016/j.scitotenv.2006.09.003
  31. Moon, C.J., Koh, H.J., Shin, I.C., Lee, E.H. and Moon, H.R. (2004) The effects of oxidative stress induced by aluminum on cellular macromolecules in the hippocampus and cerebral cortes of rats. Journal of Toxicology and Public Health, 20: 213-223.
  32. Moore, M.N. (1988) Cytochemical responses of the lysosomal system and NADPH-ferrihemoprotein reductase in molluscan digestive cells to environmental and experimental exposure to xenobiotics. Marine Ecology Progress Series, 46: 81-89. https://doi.org/10.3354/meps046081
  33. Moore, M.N., Allen, J.I. and Somerfield, P.J. (2006) Autophagy: Role in surviving environmental stress. Marine Environmental Research, 62: 420-425. https://doi.org/10.1016/j.marenvres.2006.04.055
  34. NRC (National Research Council) (1983) Risk assessment in the federal government: managing the process. National Academy Press, Washington, D.C., pp. 192.
  35. Owen, G. (1972) Lysosomes, peroxisomes and bivalves. Science Progress, 60: 299-318.
  36. Park, J.J., Park, J.C., Kim, S.S., Cho, H.S., Lee, Y.G. and Lee, J.S. (2009) Histological response of digestive gland of Gomphina veneriformis with TBTCl accumulation in sediment and soft tissue. Journal of Environmental Toxicology, 24: 341-350.
  37. Quinn, B., Gagne, F., Costello, M., McKenzie, C., Wilson, J. and Mothersill, C. (2004) The endocrine disrupting effect of municipal effluent on the zebra mussel, Dreissena polymorpha. Aquatic Toxicology, 66: 279-292. https://doi.org/10.1016/j.aquatox.2003.10.007
  38. Ra, K., Kim, K.T., Lee, J.M., Bang, J.H. and Kim, E.S. (2011) Study on heavy metals in bivalve and fish collected from lake Shihwa and outer sea. Korean Association of Ocean Science and Technology Societies, 6: 2276-2285.
  39. Rand, G.M. and Petrocelli, S.R. (1985) Introduction. In: Fundamentals of aquatic toxicology: Methods and applications. (ed. by Rand, G.M. and Petrocelli, S.R.), Hemisphere Publishing Corporation, Washington, D.C., pp. 28.
  40. Schintu, M., Durante, L., Maccioni, A., Meloni, P., Degetto, S. and Contu, A. (2008) Measurement of environmental trace-metal levels in Mediterranean coastal areas with transplanted mussels and DGT techniques. Marine Pollution Bulletin, 57: 832-837. https://doi.org/10.1016/j.marpolbul.2008.02.038
  41. Soto, M., Cajaraville, M.P. and Marigomez, I. (1996) Tissue and cell distribution of copper, zinc and cadmium in the mussel, Mytilus galloprovincialis, determined by autometallography. Tissue and Cell, 28: 557-568. https://doi.org/10.1016/S0040-8166(96)80058-9
  42. Stasiunaite, P. (1999) Long-term heavy metal mixture toxicity to embryos and alevins of rainbow trout (Oncorhynchus mykiss). Acta Zoologica Lituanica, 9: 40-46. https://doi.org/10.1080/13921657.1999.10512286
  43. Szefer, P., Kim, B.-S., Kim, C.-K., Kim, E.-H. and Lee, C.-B. (2004) Distribution and coassociations of trace elements in soft tissue and byssus of Mytilus galloprovincialis relative to the surrounding seawater and suspended matter of the southern part of the Korean Peninsula. Environmental Pollution, 129: 209-228. https://doi.org/10.1016/j.envpol.2003.10.012
  44. WWF (World Wide Fund for Nature) (2006) Reducing your risk from EDCs. W.W.F., Switzerland, pp. 20.
  45. Zorita, I., Oritiz-Zarragoitia, M., Soto, M. and Cajaraville, M.P. (2006) Biomarkers in mussels from a copper site gradient (Visnes, Norway): An integrated biochemical, histochemical and histological study. Aquatic Toxicology, 78S: S109-S116.
  46. 식품의약품안전청 (2012) 식품공전. 제 I권. 식품의약품안전청, 서울, pp. 277

Cited by

  1. Histopathologic Observation of the Mediterranean Mussel, Mytilus galloprovincialis (Lamarck, 1819) During a Spawning Season vol.36, pp.2, 2014, https://doi.org/10.4217/OPR.2014.36.2.121
  2. Concentration of Trace Metals, Intersexuality and Histological Alterations of Ruditapes philippinarum from Gamak Bay in Korea vol.31, pp.2, 2015, https://doi.org/10.9710/kjm.2015.31.2.137
  3. Systematic Review of Heavy Metal Concentrations in Fish and Shellfish in Korea vol.41, pp.1, 2018, https://doi.org/10.12771/emj.2018.41.1.1
  4. 베트남 하롱베이 이매패류 2종의 intersexuality vol.29, pp.4, 2013, https://doi.org/10.9710/kjm.2013.29.4.377
  5. 사육환경에 따른 이매패류 (Crassostrea gigas, Mytilus galloprovincialis)의 외부형질 성장과 Heat Shock Protein 70 유전자 발현 vol.49, pp.1, 2013, https://doi.org/10.11614/ksl.2016.49.1.022
  6. 한국에서 이매패류 5종의 이성생식세포 발현 vol.32, pp.3, 2016, https://doi.org/10.9710/kjm.2016.32.3.197
  7. Past, present, and future for the study of Gamak Bay, Korea vol.23, pp.3, 2013, https://doi.org/10.7846/jkosmee.2020.23.3.148
  8. Induction of sex ratio change and intersex in Tegillarca granosa (Bivalvia: Arcidae) by waterborne zinc exposure vol.21, pp.None, 2021, https://doi.org/10.1016/j.aqrep.2021.100794