The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
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Evaluation of QuEChERS Method for Determination of pesticide Residues Using GC/NPD and GC/ECD

GC/NPD와 GC/ECD를 이용한 잔류농약 정량 분석법으로써의 QuEChERS의 평가

  • Cho, Tae Hee (Kangnam Agro-Fishery Products Inspection Center, Seoul Metropolitan Goverment Research Institute of Public Health and Environment) ;
  • Park, Young Hye (Kangnam Agro-Fishery Products Inspection Center, Seoul Metropolitan Goverment Research Institute of Public Health and Environment) ;
  • Park, Hye Won (Kangnam Agro-Fishery Products Inspection Center, Seoul Metropolitan Goverment Research Institute of Public Health and Environment) ;
  • Hwang, Lae Hwong (Kangnam Agro-Fishery Products Inspection Center, Seoul Metropolitan Goverment Research Institute of Public Health and Environment) ;
  • Cho, In Soon (Kangnam Agro-Fishery Products Inspection Center, Seoul Metropolitan Goverment Research Institute of Public Health and Environment) ;
  • Kim, Min Jung (Kangnam Agro-Fishery Products Inspection Center, Seoul Metropolitan Goverment Research Institute of Public Health and Environment) ;
  • Kim, Hyun Jeong (Kangnam Agro-Fishery Products Inspection Center, Seoul Metropolitan Goverment Research Institute of Public Health and Environment) ;
  • Kim, Mu Sang (Kangnam Agro-Fishery Products Inspection Center, Seoul Metropolitan Goverment Research Institute of Public Health and Environment) ;
  • Chae, Young Zoo (Kangnam Agro-Fishery Products Inspection Center, Seoul Metropolitan Goverment Research Institute of Public Health and Environment)
  • 조태희 (강남농수산물검사소 서울특별시보건환경연구원) ;
  • 박영혜 (강남농수산물검사소 서울특별시보건환경연구원) ;
  • 박혜원 (강남농수산물검사소 서울특별시보건환경연구원) ;
  • 황래홍 (강남농수산물검사소 서울특별시보건환경연구원) ;
  • 조인순 (강남농수산물검사소 서울특별시보건환경연구원) ;
  • 김민정 (강남농수산물검사소 서울특별시보건환경연구원) ;
  • 김현정 (강남농수산물검사소 서울특별시보건환경연구원) ;
  • 김무상 (강남농수산물검사소 서울특별시보건환경연구원) ;
  • 채영주 (강남농수산물검사소 서울특별시보건환경연구원)
  • Received : 2012.11.22
  • Accepted : 2013.02.01
  • Published : 2013.03.31
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Abstract

The modified QuEChERS method was evaluated for rapid determination of pesticide residues in spinach by gas chromatography-nitrogen phosphorous detector (NPD) and electron capture detector (ECD). Fifty GC-amenable pesticides which were most frequently detected in monitoring were selected in the current study. Matrix-matched calibration was performed. The detector response for all pesticides was linear with determination coefficients higher than 0.995. LODs for most compounds ranged between 0.001 and 0.1 ug/g, and about 90% of the compounds had LODs of less than 0.05 ug/g. LOQs ranged from 0.001 to 0.32 ug/g, which were well below the MRLs permitted for most of pesticides. In the majority of cases, the recoveries (80-120%) and relative standard deviations (RSDs) (less than 20%) were within acceptable levels except for dichlorvos, propamocarb, chlorothalonil, dichlofluanid, cyhalothrin and fenvalerate. Also, this method which is applied to routine samples showed good results when comparing with traditional multi-residue method.

GC-NPD와 GC-ECD를 사용하여 잔류농약을 신속하게 정량 할 수 있는 변형 된 QuEChERS법이 시금치를 이용하여 검토되었다. 본 연구에서는 잔류농약 모니터링에서 주로 검출되며 GC로 측정 가능한 50가지 농약을 대상으로 하였다. 무농약 시금치를 이용하여 검량선을 작성하였으며, R2 값은 0.995 이상으로 모든 농약이 직선상을 나타내었다. 분석 농약의 검출한계는 0.001~0.1 ug/g로 나타났으며, 분석농약의 90% 이상이 0.05 ug/g 이하를 나타내었다. 정량한계는 0.001~0.32 ug/g로, 이는 농약의 잔류 허용기준치 보다 낮았다. Dichlorvos, propamocarb, chlorothalonil, dichlofluanid, cyhalothrin 그리고 fenvalerate를 제외하고는 대부분의 경우 회수율은 80~120%, RSD는 20% 이하로 나타났다. QuEChers는 기존 다중분석법과 비교해볼 때 일상적인 분석 시료에 응용했을 때 용매 사용량을 획기적으로 줄임으로써 친환경적 일뿐만 아니라 경제적이며 분석시간의 단축을 통해 유통 농산물의 안전성 확보에 기여할 수 있는 바가 크다고 할 수 있겠다.

Keywords

References

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