Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

Anti-wrinkle Activity of a Curdrania tricuspidata Extract on Ultraviolet-induced Photoaging

Hairless Mice를 이용한 광노화 모델에서 꾸지뽕나무 추출물의 피부주름 형성 억제효과

  • Kim, Ok Kyung (Dept. of Medical Nutrition, Kyung Hee University) ;
  • Ho, Jin-Nyoung (Dept. of Medical Nutrition, Kyung Hee University) ;
  • Nam, Da-Eun (Dept. of Medical Nutrition, Kyung Hee University) ;
  • Jun, Woojin (Dept. of Food and Nutrition, Chonnam National University) ;
  • Lee, Jeongmin (Dept. of Medical Nutrition, Kyung Hee University)
  • 김옥경 (경희대학교 의학영양학과) ;
  • 호진녕 (경희대학교 의학영양학과) ;
  • 남다은 (경희대학교 의학영양학과) ;
  • 전우진 (전남대학교 식품영양학과) ;
  • 이정민 (경희대학교 의학영양학과)
  • Received : 2012.12.06
  • Accepted : 2013.01.11
  • Published : 2013.04.30
Download PDF
⟨ Previous Next ⟩

Abstract

We investigated the anti-wrinkle activity of an 80% ethanol extract of Curdrania tricuspidata leaves (CTL80) on ultraviolet-induced photoaging in hairless mice. Skin wrinkles were induced by 10 weeks of UVB-irradiation on the back of Skh-1 hairless mice three times a week. Mice were divided into ten groups; normal control (-UVB), UVB irradiated control group (+UVB), dietary groups (UVB+ascorbic acid 0.1%, UVB+CTL80 0.1%, UVB+CTL80 0.25%) and topical application groups (-UVB+base lotion (BL), UVB+BL, UVB+ascorbic acid 1%+BL, UVB+CTL80 1%+BL, UVB+CTL80 2%+BL). Wrinkle formation, histological changes, superoxide dismutase (SOD) activities, glutathione peroxidase (GSH-Px), and the expression of matrix metalloproteinases (MMP-1, MMP-3 and MMP-9) were analyzed. Wrinkles for the +UVB groups formed as a pattern of deep furrows and thick crests. Wrinkles with CTL80 treatment formed as a pattern of shallow furrows and thin crests, with wrinkle areas were lower than the +UVB group. In an antioxidant analysis of mouse blood, SOD and GSH-Px activities were significantly higher in the CTL80 topical application group compared to the +UVB group. The mRNA expression of MMPs in the +UVB group was significantly higher than the normal control group, and significantly lower in the CTL80-treated group. In conclusion, CTL80 exerted anti-wrinkle activity on ultraviolet-induced photoaging by regulating antioxidative defense systems and MMPs expression.

본 실험에서는 안전하고 우수한 효과를 지닌 천연항산화제의 광노화에 의한 피부주름 형성을 억제하는 천연물질을 탐색하기 위한 연구의 일환으로 CTL80의 식이투여와 로션 도포가 광노화를 유발한 동물모델에서 주름 형성에 미치는 영향을 살펴보았다. 광노화 유발 동물모델을 만들기 위해 hairless mice에 10주 동안 UVB 조사를 주 3회 실시하였다. 형태학적 변화를 관찰한 결과 UVB를 조사한 군은 각질이 증가하고 주름 능선의 두께가 굵고 주름이 깊게 형성한 반면, 양성대조군인 비타민 C와 CTL80 처리한 군에서 주름 형성이 감소하였으며, 특히 2% CTL80 로션 도포군이 가장 주름 형성을 억제하였다. 피부조직의 H&E 염색을 통한 조직학적인 변화에서는 UVB 조사로 두꺼워진 각질층과 표피층을 관찰하였으며 진피층의 교원섬유가 감소되었음이 관찰되었다. CTL80은 각질층과 표피층의 증가를 감소시켰으며 진피층의 교원섬유를 증가시켰음을 관찰하였다. 형태학적 변화와 같이 2% CTL80 로션 도포군의 효과가 가장 양호하게 나타났다. 항산화 상태에 미치는 영향을 살펴보기 위해 동물 혈청의 SOD와 GSH-Px의 활성을 측정한 결과, 비타민C와 CTL80처리 군에서 UVB만 조사한 군에 비하여 활성이 유의적으로 높아졌음을 살펴볼 수 있었다. 항산화 활성 또한 CTL80에 농도 의존적이지는 않았으나 식이 투여군에 비하여 로션 도포군에서 높은 활성을 보였다. 교원질 합성을 억제하고 분해를 촉진시키는 MMPs(MMP-1, MMP-3, MMP-9)의 발현을 실시간 정량 PCR로 측정하여 발현 변화를 살펴보았다. 그 결과 식이 투여군과 로션 도포군 모두 UVB 조사군에서는 발현이 현저히 높아졌으나 비타민 C와 CTL80 처리 군에서 유의적으로 낮아졌음을 살펴볼 수 있었다. 특히 CTL80 로션 도포군에서 MMP-9의 발현이 확연히 낮아졌음을 관찰할 수 있었다. 이러한 결과를 통하여 CTL80은 높은 항산화 활성을 지녔으며 이러한 항산화능을 통하여 UVB로 광노화를 유발시킨 동물모델에서 주름 생성 억제에 긍정적인 영향을 미쳤고 주름 억제 효능을 지니는 기능성 소재로써 개발 가능성을 확인하였다.

Keywords

References

  1. Rittie L, Fisher GJ. 2002. UV-light-induced signal cascades and skin aging. Ageing Research Reviews 1: 705-720. https://doi.org/10.1016/S1568-1637(02)00024-7
  2. Bernerd F, Vioux C, Asselineau D. 2000. Evaluation of the protective effect of sunscreens on in vitro reconstructed human skin exposed to UVB or UVA irradiation. Photochem Photobiol 71: 314-320. https://doi.org/10.1562/0031-8655(2000)071<0314:EOTPEO>2.0.CO;2
  3. Yasui H, Sakurai H. 2003. Age-dependent generation of reactive oxygen species in the skin of live hairless rats exposed to UVA light. Exp Dermatol 12: 655-661 https://doi.org/10.1034/j.1600-0625.2003.00033.x
  4. Ansel JC, Luger TA, Lowry D, Perry P, Roop DR, Mountz JD. 1988. The expression and modulation of IL-$1{\alpha}$ in murine keratinocytes. J Immunol 140: 2274-2278.
  5. Castanet J, Ortonne JP. 1997. Pigmentary changes in aged and photoaged skin. Arch Dermatol 133: 1296-1299. https://doi.org/10.1001/archderm.1997.03890460120015
  6. Fisher GJ, Wang ZQ, Datta SC, Varani J, Kang SW, Voorhees JJ. 1997. Pathophysiology of premature skin aging induced by ultraviolet light. New Eng J Med 337: 1419-1428. https://doi.org/10.1056/NEJM199711133372003
  7. Shephard P, Martin G, Smola-Hess S, Brunner G, Krieg T. Smola H. 2004. Myofibroblast differentiation is induced in keratinocyte-fibroblast co-cultures and is antagonistically regulated by endogenous transforming growth factor-${\beta}$ and interleukin-1. Am J Pathol 164: 2055-2066. https://doi.org/10.1016/S0002-9440(10)63764-9
  8. Steenvoorden DP, Henegouwen GM. 1997. The use of endogenous antioxidants to improve photoprotection. J Photochem Photobiol B 41: 1-13. https://doi.org/10.1016/S1011-1344(97)00081-X
  9. Darr D, Fridovich I. 1994. Free radicals in cutaneous biology. J Invest Dermatol 102: 671-675. https://doi.org/10.1111/1523-1747.ep12374036
  10. Masteikova R, Muselik J, Bernatoniene J, Majiene D, Savickas A, Malinauskas F, Bernatoniene R, Peciura R, Chalupova Z, Dvorackova K. 2008. Antioxidant activity of tinctures prepared from hawthorn fruits and motherwort herb. Ceska Slov Farm 57: 35-38.
  11. Kim OK, Ho JN, Nam DE, Jun WJ, Hwang KT, Kang JE, Chae OS, Lee JM. 2012. Hepatoprotective effect of Curdrania tricuspidata extract against oxidative damage. J Korean Soc Food Sci Nutr 41: 7-13. https://doi.org/10.3746/jkfn.2012.41.1.007
  12. Kim JY, Chung JH, Hwang I, Kwan YS, Chai JK, Lee KH, Han TH, Moon JH. 2009. Quantification of quercetin and kaempferol contents in different parts of Cudrania tricuspidata and their processed foods. Kor J Hort Sci Technol 27: 489-496.
  13. Choi SR, You DH, Kim JY, Park CB, Kim DH, Ryu J. 2009. Antioxidant activity of methanol extracts from Curdrania tricuspidata Bureau according to harvesting parts and time. Korean J Med Crop Sci 17: 115-120.
  14. Oh PS, Lim KT. 2011. Anti-inflammatory effect of glycoprotein isolated from Cudrania tricuspidata Bureau: involvement of MAPK/NF-${\kappa}B$ signaling. Immunol Invest 40:76-91. https://doi.org/10.3109/08820139.2010.516049
  15. Kang DG, Hur TY, Lee GM, Oh H, Kwon TO, Sohn EJ, Lee HS. 2002. Effects of Cudrania tricuspidata water extract on blood pressure and renal functions in NO-dependent hypertension. Life Sci 70: 2599-2609. https://doi.org/10.1016/S0024-3205(02)01547-3
  16. Lee IK, Song KS, Kim CJ, Kim HM, Oh GT, Yoo ID. 1994. Tumor cell growth inhibition and antioxydative activity of flavonoids from the stem bark of Cudrania tricuspidata. Agric Chem Biotechnol 37: 105-109.
  17. Chung JH. 2003. Photoaging in asians. Photodermatol Photoimmunol Photomed 19: 109-121. https://doi.org/10.1034/j.1600-0781.2003.00027.x
  18. Kligman LH. 1996. The hairless mouse model for photoaging. Clin Dermatol 14: 185-195.
  19. Seite S, Zucchi H, Septier D, Igondjo-Tchen S, Senni K, Godeau G. 2006. Elastin changes during chronological and photo-ageing: the important role of lysozyme. J Eur Acad Dermatol Venereol 20: 980-987.
  20. Woods GM, Malley RC, Muller HK. 2005. The skin immune system and the challenge of tumour immuno-surveillance. Eur J Dermatol 15: 63-69.
  21. Deisseroth A, Dounce AL. 1970. Catalase physical and chemical properties, mechanism of catalysis and physiological role. Physiol Rev 50: 3-24.
  22. Halliwell B, Gutteridge JMC. 1998. Free radicals in biology and medicine. Oxford University Press, London, UK. p 32.
  23. Kirnbaner R, Kock A, Neuner P, Forster E, Krutmann J, Urbanski A, Schauer E, Ansel JC, Schwarz T, Luger TA. 1991. Regulation of epidermal cell interleukin-6 production by UV light and corticosteroids. J Invest Dermatol 96:484-489. https://doi.org/10.1111/1523-1747.ep12470181
  24. Ikeda T, Tsutsumi T. 1990. Function and skin depigmental activity of crude drugs. Fragrance J 6: 59-66.
  25. Talwar HS, Griffiths CEM, Fisher GJ, Hamilton TA, Voorhees JJ. 1995. Reduced type I and type III procollagens in photodamaged adult human skin. J Invest Dermatol 105:285-290. https://doi.org/10.1111/1523-1747.ep12318471

Cited by

  1. Consideration of preservation methods for plant genetic resources in natural monument - Focusing on preparation for becoming effective of Nagoya Protocol - vol.41, pp.3, 2014, https://doi.org/10.7744/cnujas.2014.41.3.193
  2. Cytoprotective effects against UVA and physical properties of luteolin-loaded cationic solid lipid nanoparticle vol.35, 2016, https://doi.org/10.1016/j.jiec.2015.12.014
  3. Characteristics and Cellular Protective Effects against UVA of Cationic Liposome Loaded with Quercetin and Rutin vol.26, pp.2, 2015, https://doi.org/10.14478/ace.2015.1002
  4. Effects of Cudrania tricuspidata Fruit on the Performance, Blood Metabolites and IgG in Laying Hens vol.43, pp.1, 2016, https://doi.org/10.5536/KJPS.2016.43.1.15
  5. 꾸지뽕나무 추출물의 피부 생리 활성 vol.32, pp.2, 2013, https://doi.org/10.12925/jkocs.2015.32.2.260
  6. 꾸지뽕나무 추출물의 피부 생리 활성 vol.32, pp.2, 2013, https://doi.org/10.12925/jkocs.2015.32.2.260
  7. Anti-Inflammatory Effects of Compounds from Cudrania tricuspidata in HaCaT Human Keratinocytes vol.22, pp.14, 2013, https://doi.org/10.3390/ijms22147472
  8. Effects of Sargassum thunbergii Extract on Skin Whitening and Anti-Wrinkling through Inhibition of TRP-1 and MMPs vol.26, pp.23, 2013, https://doi.org/10.3390/molecules26237381