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Anti-inflammatory and Anti-allergic Effects of Herbal Extracts on Atopic Dermatitis ( Part II )

약용식물 추출물의 아토피성 피부염에 대한 항염증 및 항알레르기 효과 (제 2 보)

  • 랑문정 (배재대학교 분자과학부)
  • Received : 2013.02.26
  • Accepted : 2013.03.25
  • Published : 2013.03.30

Abstract

Atopic dermatitis is a chronic, relapsing inflammatory skin disease associated with dysfunction of skin barrier and cutaneous hyper-reactivity to environmental triggers. In the previous study, cytotoxicity, antioxidant, anti-inflammatory and anti-allergic activities were investigated for various herbal extracts such as Aloe vera L. (AV), Viola mandshurica W. Becker (VM), Punica granatum L. (PG), and Dendrobium nobile L. (DN) in order to develop effective therapeutic herbal extracts for atopic dermatitis, In this study, anti-inflammatory activities of these herb extracts in lipopolysaccharide (LPS)-induced macrophage RAW264.7 cells were further examined to find the underlying molecular mechanisms. The RT-PCR (reverse transcription polymerase chain reaction) analysis showed that PG, DN and AV inhibited effectively the gene expression of pro-inflammatory cytokines IL-6 and IL-$1{\beta}$ in LPS-stimulated macrophages, while VM did not. The transfection and luciferase analysis exhibited that all herbal extracts hindered the activation of transcription nuclear factor kappa B (NF-${\kappa}B$). The western blot analysis indicated that AV blocked the activation of only JNK MAP (c-Jun N-terminal kinase mitogen-activated protein) kinase not p38 MAP kinase, while VM, PG and DN did not show the activation of both JNK and p38 MAP kinases. These results suggest that AV, VM, PG, and DN have anti-inflammatory activities and thus have the potential to reduce and alleviate the symptoms of atopic dermatitis.

아토피성 피부염은 만성 재발성 염증성 피부질환으로 피부장벽기능의 이상과 환경유발인자에 대한 피부과민성과 연관되어 있다. 이전의 연구에서는 아토피성피부염에 효과적인 약용식물 추출물을 발굴하기 위하여 노회, 자회지정, 석류, 석곡 추출물들의 세포독성, 항산화, 항염, 항알레르기 효과를 검토하였다. 본 연구에서는 지질다당류로 활성화시킨 대식세포 RAW26.7 에 대한 약용식물 추출물들의 항염작용을 보다 상세하게 검토하여 항염작용의 근본적인 분자기전을 확인하고자 하였다. 역전사중합효소연쇄반응분석(reverse transcription polymerase chain reaction analysis) 결과, 석류, 석곡, 노회는 염증성 사이토카인인 IL-6 와 IL-$1{\beta}$ 유전자발현을 현저하게 억제시켰으며 자화지정은 영향이 없었다. 형질주입과 발광효소분석(transfection and luciferase analysis) 결과, 약용식물 모두가 전사 핵인자 카파비(NF-${\kappa}B$)의 활성화를 억제시켰다. 웨스턴 블럿 분석(western blot analysis) 결과, 노회는 JNK MAP 인산화효소의 활성화를 차단하였지만 p38 MAP 인산화효소의 활성화는 차단하지 못하였다. 반면에 자화지정, 석류, 석곡은 JNK MAP 인산화효소뿐만 아니라 p38 MAP 인산화효소의 활성화도 차단하였다. 이들 실험결과들은 노회, 자화지정, 석류, 석곡은 항염효능을 가지고 있으며 따라서 아토피성 피부염의 증상을 경감 또는 완화시키는 잠재력이 있음을 보여 준다.

Keywords

References

  1. K. Wolff, et al., "Fitzpatrick's Dermatology in General Medicine", 7th ed., p.146, McGraw-Hill, New York (2008).
  2. M. J. Rang, Anti-inflammatory and Anti-allergic Effects of Herbal Extracts on Atopic Dermatitis (Part I), J. of the Korean Oil Chemists' Soc. , 28(1), 75 (2011).
  3. Y. S. Kang, K. Y. Kyeong, M. J. Rang, D. H. Hwan, Y. K. Lee, W. K. Cho, S K. Choi, and S. K. Han, "Science of Cosmetics and Health Care Product", vol 1, p. 325, Shinkwang Publisher, Seoul (2008).
  4. Textbook Compilation Committee of Korean Dermatological Association, "Dermatology", 5th ed., p.165, Yeomungak, Seoul (2008).
  5. K. S. Li, ABCs of Atopic Dermatitis, The Journal of Skin barrier Research, 13(1), 28 (2011).
  6. H. J. Kwon, Is Atopic Dermatitis an Environmental Disease ?, The Journal of Skin barrier Research, 14(2), 28 (2012).
  7. M. J. Rang, Anti-inflammatory and Anti-allergic Effects of Herbal Extracts on Atopic Dermatitis (Part I), J. of the Korean Oil Chemists' Soc., 28(1), 75 (2011).
  8. S. Bonness and T. Bieber, Molecular Basis of Atopic Dermatitis, Curr. Opin. Allergy Clin. Immunolol., 7, 382 (2007). https://doi.org/10.1097/ACI.0b013e3282a643c3
  9. T. Bieber, Atopic Dermatitis, N. Engl. J. Med., 358, 1483 (2008). https://doi.org/10.1056/NEJMra074081
  10. W. Abramovits, Atopic Dermatitis, J. Am. Acad. Dermatol., 53, S86 (2005). https://doi.org/10.1016/j.jaad.2005.04.034
  11. N. Novak and T. Bieber, The Role of Dentric Cell Subtypes in the Pathophysiology of Atopic Dermatitis, J. Am. Acad. Dermatol., 53, S171 (2005). https://doi.org/10.1016/j.jaad.2005.04.060
  12. J. W. Cho, Current Opinions in Pathogenesis of Atopic Dermatitis : Skin Barrier, Inflammation, and Microbiome, The Journal of Skin barrier Research, 14(1), 55 (2012).
  13. S. J. Seo, Genetic Factors of Atopic Dermatitis, The Journal of Skin barrier Research, 14(2), 20 (2012).
  14. C. N. A. Palmer, et al., Common Loss-of-Function Variants of the Epidermal Barrier Protein Filaggrin are a Major Predisposing Factor for Atopic Dermatitis, Nature Genetics, 38(4), 441 (2006). https://doi.org/10.1038/ng1767
  15. G. M. O'Regan, A. Sandilands, W. H. Irwin Mclean, and A.D. Irvine, Filaggrin in Atopic Dermatitis, J. Allergy Clin. Immunolol., 122, 689 (2008). https://doi.org/10.1016/j.jaci.2008.08.002
  16. A. Irvine, W. H. Irwin Mclean, and D. Leung, Filaggrin Mutations Associated with Skin and Allergic Diseases Atopic Dermatitis, N. Engl. J. Med., 365, 1315 (2011). https://doi.org/10.1056/NEJMra1011040
  17. M. A. McAller and A.D. Irvine, The Multifuctional Role of Filaggrin in Allergic Skin Disease, J. Allergy Clin. Immunolol., 131, 280 (2013). https://doi.org/10.1016/j.jaci.2012.12.668
  18. Y. L. Chung and S. H. Lee, Atopic Dermatitis, The Journal of Skin barrier Research, 3, 40 (2001).
  19. J, R. Lee, Topical Treatment of Atopic Dermatitis, The Journal of Skin barrier Research, 14(1), 58 (2012).
  20. T. Horikawa and M. Ichihashi, Efficacy of Glycoceramide-containing Cream and Lotion on Atopic Dermatitis, Fragrance Journal, 14(10), 29 (1999) https://doi.org/10.1002/(SICI)1099-1026(199901/02)14:1<29::AID-FFJ765>3.0.CO;2-G
  21. B. D. Park,J. K. Youm, S. K. Jeong, E. H. Choi, S. K. Ahn, and S. H. Lee, The Characterization of Molecular Organization of Multilamellar Emulsions Containing Pseudoceramide and Type III Synthetic Ceramide, J. Invest. Dermatol., 121, 794 (2003). https://doi.org/10.1046/j.1523-1747.2003.12470.x
  22. H. Tagami, Treatment of the Atopic Dermatitis Skin and Skin Care, Fragrance Journal, 18(6), 13 (2003). https://doi.org/10.1002/ffj.1134
  23. H. Tamura and T. Kinokuni, Skin Care Ingredients for Atopic Dermatitis, Fragrance Journal, 18(6), 60 (2003).
  24. E. Simpson, Atopic Dermatitis: A Review of Topical Treatment Options, Current Medical Research and Opinion, 26(3), 633 (2010). https://doi.org/10.1185/03007990903512156
  25. M. J. Rang, Anti-inflammatory and Antiallergic Effects of Herbal Extracts on Atopic Dermatitis (Part I), J. of the Korean Oil Chemists' Soc., 28(1), 75 (2011).
  26. H. W. Jung and Y. K. Park, Inhibitory Effect of Coptidis Rhizoma on LPSinduced Production of Nitric Oxide and TNF-$\alpha$ in Mouse Macrophages, Kor. J. Herbology, 21(2), 165 (2006).
  27. D. K. Choi, Y. S. Lee, K. M. Lee, K. H. Kim, M. J. Cho, C. D. Kim, and J. H. Lee, Effect of X-ray on the Expression of p53 in Human Skin Tissue Cultured in Vitro, Korean Journal of Investigative Dermatology, 14(2), 52 (2007).
  28. J. H. Lee, E.Y. Seo, E. K. Jeon, C. D. Kim, J. H. Lee, and H. J. Song, Identification of the Genes Differentially Expressed in the Keratinocytes by Treatment with 12-O Tetradecanoylphorbol-13- Acetate Using Suppression Subtractive Hybridization, Korean Journal of Investigative Dermatology, 14(1), 17 (2007).
  29. T. K. Means, R. P. Pavlovich, D. Roca, M. W. Vermeulen, and M. J. Fenton, Activation of TNF-$\alpha$ Transcription Utilizes Distinct MAP Kinase Pathways in Different Macrophage Populations, J. Leukoc. Biol.. 67, 885 (2000).
  30. D. K. Choi, Y. S. Lee, K. M. Lee, K. H. Kim, M. J. Cho, C. D. Kim, and J. H. Lee, Effect of X-ray on the Expression of p53 in Human Skin Tissue Cultured in Vitro, Korean Journal of Investigative Dermatology, 14(2), 52 (2007)
  31. D. H. Kim, H. S. Yi, H. J. Yun, C. M. Cha, and S. D. Park, Anti-inflammatory Effect of Methanol Extract of Keum- Ryung-Ja-San in Mouse Macrophages, Kor. J. Herbology, 25(2), 89 (2010).
  32. M. J. Rang, Anti-inflammatory and Antiallergic Effects of Herbal Extracts on Atopic Dermatitis (Part I), J. of the Korean Oil Chemists' Soc. , 28(1), 75 (2011).
  33. M. Y. K. Leung, C. Liu, L. F. Zhu, Y. Z. Hui, B. Yu, and K. P. Fung, Chemical and Biological Characterization of a Polysaccharide Biological Response Modifier from Aloe vera L. var. chinensis (Haw.) Berg., Glycobiology, 14(6), 501 (2004). https://doi.org/10.1093/glycob/cwh050
  34. S. A. Im, S. T. Oh, S. G. Song, M. R. Kim, D. S. Kim, S. S. Woo, T. H. Jo, Y. I. Park, and C. K. Lee, Identification of Optimal Molecular Size of Modified Aloe Polysaccharides with Maximum Immunomodulatory Activity. International Immunopharmacology, 5, 271 (2005). https://doi.org/10.1016/j.intimp.2004.09.031
  35. M. J. Rang, Anti-inflammatory and Antiallergic Effects of Herbal Extracts on Atopic Dermatitis (Part I), J. of the Korean Oil Chemists' Soc. , 28(1), 75 (2011).
  36. P. A. Baeuerle and T. Henkel, Function and Activation of NF-kappa B in the Immune System, Annu. Rev. Immunol., 141 (1994).
  37. Y. Liang, Y. Zhou, and P. P. Shen, NF- $\kappa$B and Its Regulation on the Immune System, Cellular & Molecular Immunology, 1(5), 343 (2004).
  38. J. Y. Huh, H. J. Cho. K. J. Park, and S. D. Park, Anti-inflammatory Effect of Rumex japonicus HOUTT. in RAW 264.7 Cells, Kor. J. Herbology, 27(4), 99 (2012).
  39. J. Y. Huh, H. J. Cho. K. J. Park, and S. D. Park, Anti-inflammatory Effect of Rumex japonicus HOUTT. in RAW 264.7 Cells, Kor. J. Herbology, 27(4), 99 (2012).
  40. M. S. Kim, J. S. Jeong, H. Y. Lee, Y. S. Ju , G. S. Bae, S. W. Seo, I. J. Cho, S. J. Park, and H. J. Song, Anti-oxidative Effects of Dendrobii Herba on Toxic Agent Induced Kidney Cell Injury, Kor. J. Herbology, 26(2), 51 (2011).
  41. C. Dinarello, Proinflammatory Cytokines, Chest, 118(2), 503 (2000). https://doi.org/10.1378/chest.118.2.503
  42. S. R. Chen, X. Z. Xu, Y. H. Wang, J. W. Chen, S. W. Xu, L. Q. Gu, and P. Q. Liu. Icariin Derivative Inhibits Inflammation Through Suppression of p38 Mitogenactivated Protein Kinase and Nuclear factor-kappa B Pathways. Biol Pharm Bull., 33(8), 1307 (2010). https://doi.org/10.1248/bpb.33.1307
  43. S. Tang, X. Y. Shen, H. Q. Huang, S. W. Xu, Y. Yu, C. H. Zhou, S. R. Chen, K. Le, Y. H. Wang, and P. Q. Liu. Cryptotanshinone Suppressed Inflammatory Cytokines Secretion in RAW264.7 Macrophages Through inhibition of the NF-$\kappa$B and MAPK Signaling Pathways. Inflammation. 34(2), 111 (2011) https://doi.org/10.1007/s10753-010-9214-3

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