Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
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Effects of medicinal herb water extracts on expression of hepatic glucokinase, pyruvate dehydrogenase and acetyl-CoA carboxylase mRNA

한약재 물 추출물이 간세포 Glucokinase, Pyruvate Dehydrogenase, Acetyl-CoA Carboxylase mRNA 발현에 미치는 영향

  • Kim, Hyun Sook (Well-being Bioproducts RIC Center, Kangwon National University) ;
  • Kim, Tae Woo (Well-being Bioproducts RIC Center, Kangwon National University) ;
  • Kim, Dae Jung (Well-being Bioproducts RIC Center, Kangwon National University) ;
  • Lee, Jae Sung (Department of Bio-Health Technology, Kangwon National University) ;
  • Choe, Myeon (Well-being Bioproducts RIC Center, Kangwon National University)
  • 김현숙 (강원대학교 웰빙특산물산업화지역혁신센터) ;
  • 김태우 (강원대학교 웰빙특산물산업화지역혁신센터) ;
  • 김대중 (강원대학교 웰빙특산물산업화지역혁신센터) ;
  • 이재성 (강원대학교 생명건강공학과) ;
  • 최면 (강원대학교 웰빙특산물산업화지역혁신센터)
  • Received : 2013.01.21
  • Accepted : 2013.02.28
  • Published : 2013.04.30
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Abstract

We studied the anti-diabetic effects of medicinal herb water extracts on expression of hepatic glucokinase (GCK), pyruvate dehydrogenase (PDH), and acetyl-CoA carboxylase (ACC) mRNA. The medicinal herbs used for experiments were Cornus officinalis (CO), Paeonia suffruticosa Andrews (PSA), Discorea japonica Thunb. (DJ), Rehmannia glutinosa (RG), Lycium chinense (LC), and Pyrus pyrifolia (PP). For GCK mRNA expression, CO, RG, and LC water extracts exhibited a more effective activity than other extracts. Cells treated with RG and LC water extracts showed an increase in expression of PDH mRNA to 191% and 124%, respectively, compared to control. Expression of ACC mRNA was significantly higher in LC water extract. These data indicate that CO, RG, and LC water extracts stimulates expression of hepatic GCK, PDH, and ACC mRNA.

본 연구에서는 산수유, 목단피, 산약, 숙지황, 지골피, 야생배의 한약재 물 추출물이 당대사 관련 효소인 GCK, PDH, ACC mRNA 발현정도에 미치는 영향을 측정하였다. HepG2 세포에 대한 세포독성을 측정한 결과, GCK, PDH, ACC mRNA 발현량을 측정하기 위한 물 추출물의 농도 범위는 세포 생존율에 영향을 주지 않는 100, 250, 500 ppm로 결정하였다. GCK mRNA 발현은 100 ppm에서 숙지황 물 추출물이 165%로 가장 높게 나타났고, 250 ppm에서는 숙지황과 지골피 물 추출물이 각각 180%, 154%로 높았으며, 500 ppm에서는 산수유(195%), 목단피 (157%), 야생배 (139%), 산약 (122%), 지골피 (117%), 숙지황 (113%)의 순으로 GCK mRNA 발현이 증가되었다. PDH mRNA 발현량은 250 ppm 농도에서 지골피, 숙지황 물추출물에서 각각 141%, 118% 증가되었고, 500 ppm에서는 지골피, 숙지황 물 추출물에서 각각 191%, 124% 증가되었다. ACC mRNA 발현량은 500 ppm에서 지골피 (188%), 숙지황 (126%)로 가장 높게 나타났다. 결과적으로 GCK, PDH, ACC mRNA 발현량을 증가시킬 수 있는 소재로 산수유, 숙지황, 지골피 등을 꼽을 수 있겠으며 이들 소재들은 식후 혈당상승을 억제할 수 있는 항당뇨 천연소재로 이용될 수 있음을 제시하였다.

Keywords

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