Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Comparison of the Ingredient Quantities and Biological Activities of Hwangryunhaedok-tang (Hwanglianjiedu-tang) Decoction and Commercial Extractive Granules

황련해독탕 전탕액과 시판제제의 성분 및 효능 비교

  • Kim, Yeji (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine) ;
  • Kim, Ohn Soon (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine) ;
  • Seo, Chang-Seob (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine) ;
  • Shin, Hyeun-Kyoo (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine)
  • 김예지 (한국한의학연구원, 한약기초연구그룹) ;
  • 김온순 (한국한의학연구원, 한약기초연구그룹) ;
  • 서창섭 (한국한의학연구원, 한약기초연구그룹) ;
  • 신현규 (한국한의학연구원, 한약기초연구그룹)
  • Received : 2012.12.04
  • Accepted : 2013.01.11
  • Published : 2013.03.31
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Abstract

This study was to investigate the quality of commercial Hwangryunhaedok-tang (Hwanglianjiedu-tang, HHT) extractive granules by comparing with HHT decoction. The contents of index components and the anti-inflammatory and antioxidative abilities of two different commercial HHT granules (HHT-2 and HHT-3) were compared with those of the HHT decoction (HHT-1). The contents were analyzed with HPLC. The anti-inflammatory effects were determined by measuring NO, $PGE_2$ and IL-6 in RAW 264.7 cell. We compared the anti-oxidative effects through ABTS radical scavenging activities. The range of contents of 7 ingredients was 0.42-36.54 mg/g in HHT-1, not detected-12.30 mg/g in HHT-2, not detected-18.79 mg/g in HHT-3. Although HHT-1, HHT-2, HHT-3 had the inhibitory effects on the production of NO, $PGE_2$ and the scavenging activities on ABTS, HHT-1 showed stronger effects than HHT-2, HHT-3 (HHT-1 > HHT-3 > HHT-2). HHT-1 inhibited the production of IL-6, while HHT-2, HHT-3 showed no inhibitory effects. It is necessary to find appropriate methods for extracting HHT and to establish standardized processes, in order to improve the quality of commercial traditional herbal formula.

Keywords

References

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