The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Anti-Oxidative Activity of Pectin and Its Stabilizing Effect on Retinyl Palmitate

  • Received : 2013.04.01
  • Accepted : 2013.05.07
  • Published : 2013.06.30
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Abstract

The purpose of this study was to examine the anti-oxidative activity of pectin and other polysaccharides in order to develop a cosmeceutical base having anti-oxidative effects towards retinyl palmitate (RP). The anti-oxidative stabilizing effects of pectin and other polysaccharides on RP were evaluated by DPPH assay and then the stabilizing effect of pectin on RP was examined as a function of time. Among the polysaccharides we examined, pectin exhibited a considerably higher anti-oxidative activity, with an approximately 5-fold greater DPPH radical scavenging effect compared to other polysaccharides. The DPPH radical scavenging effect of pectin increased gradually with increasing concentrations of pectin. At two different RP concentrations, 0.01 and 0.1% in ethanol, addition of pectin improved the stability of RP in a concentration dependent manner. The stabilizing effect of pectin on RP was more effective for the lower concentration of RP (0.01%, v/v). Further, degradation of RP was reduced following the addition of pectin as measured over 8 hours. From the results obtained, it can be suggested that pectin may be a promising ingredient for cosmeceutical bases designed to stabilize RP or other pharmacological agents subject to degradation by oxidation.

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References

  1. Boehnlein J, Sakr A, Lichtin JL, Bronaugh RL. Characterization of esterase and alcohol dehydrogenase activity in skin. Metabolism of retinyl palmitate to retinol (vitamin A) during percutaneous absorption. Pharm Res. 1994;11:1155- 1159. https://doi.org/10.1023/A:1018941016563
  2. Draelos ZD. The cosmeceutical realm. Clin Dermatol. 2008; 26:627-632. https://doi.org/10.1016/j.clindermatol.2007.09.005
  3. Serri R, Iorizzo M. Cosmeceuticals: focus on topical retinoids in photoaging. Clin Dermatol. 2008;26:633-635. https://doi.org/10.1016/j.clindermatol.2007.09.016
  4. Guenin EP, Zatz JL. Skin permeation of retinyl palmitate from vescicles. J Soc Cosmet Chem. 1995;46:261-270.
  5. Sorg O, Tran C, Carraux P, Grand D, Hügin A, Didierjean L, Saurat JH. Spectral properties of topical retinoids prevent DNA damage and apoptosis after acute UV-B exposure in hairless mice. Photochem Photobiol. 2005;81:830-836. https://doi.org/10.1562/2004-10-01-RA-333R1.1
  6. Antille C, Tran C, Sorg O, Carraux P, Didierjean L, Saurat JH. Vitamin A exerts a photoprotective action in skin by absorbing ultraviolet B radiation. J Invest Dermatol. 2003; 121:1163-1167. https://doi.org/10.1046/j.1523-1747.2003.12519.x
  7. Idson B. Vitamins in cosmetics, an update I. overview and vitamin A. Drug Cosmet Ind. 1990;146:26-91.
  8. Ihara H, Hashizume N, Hirase N, Suzue R. Esterification makes retinol more labile to photolysis. J Nutr Sci Vitaminol (Tokyo). 1999;45:353-358. https://doi.org/10.3177/jnsv.45.353
  9. Carlotti ME, Rossatto V, Gallarate M, Trotta M, Debernardi F. Vitamin A palmitate photostability and stability over time. J Cosmet Sci. 2004;55:233-252.
  10. Carlotti ME, Rossatto V, Gallarate M. Vitamin A and vitamin A palmitate stability over time and under UVA and UVB radiation. Int J Pharm. 2002;240:85-94. https://doi.org/10.1016/S0378-5173(02)00128-X
  11. Ihara H, Hashizume N, Hirase N, Suzue R. Esterification makes retinol more labile to photolysis. J Nutr Sci Vitaminol (Tokyo). 1999;45:353-358. https://doi.org/10.3177/jnsv.45.353
  12. Sane A, Limtrakul J. Formation of retinyl palmitate-loaded poly(l-lactide) nanoparticles using rapid expansion of supercritical solutions into liquid solvents (RESOLV). J Supercrit Fluid. 2009;51:230-237. https://doi.org/10.1016/j.supflu.2009.09.003
  13. Carlotti ME, Rossatto V, Gallarate M, Trotta M, Debernardi F. Vitamin A palmitate photostability and stability over time. J Cosmet Sci. 2004;55:233-252.
  14. Maxwell EG, Belshaw NJ, Waldron KW, Morris VJ. Pectin - An emerging new bioactive food polysaccharide. Trends Food Sci Tech. 2011;24:64-73.
  15. Wong TW, Colombo G, Sonvico F. Pectin matrix as oral drug delivery vehicle for colon cancer treatment. AAPS Pharm Sci Tech. 2011;12:201-214. https://doi.org/10.1208/s12249-010-9564-z
  16. Yamaguchi T, Takamura H, Matoba T, Terao J. HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl. Biosci Biotechnol Biochem. 1998;62:1201-1204. https://doi.org/10.1271/bbb.62.1201
  17. Rice Evans CA, Miller NJ, Paganga G. Antioxidant properties of phenolic compounds. Trends Plant Sci. 1997;2:152-159. https://doi.org/10.1016/S1360-1385(97)01018-2
  18. Rice-Evans CA, Miller NJ, Paganga G. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radic Biol Med. 1996;20:933-956. https://doi.org/10.1016/0891-5849(95)02227-9
  19. Denes JM, Baron A, Renard CM, Pean C, Drilleau JF. Different action patterns for apple pectin methylesterase at pH 7.0 and 4.5. Carbohydr Res. 2000;327:385-393. https://doi.org/10.1016/S0008-6215(00)00070-7
  20. García-Ochoa F, Santos VE, Casas JA, Gomez E. Xanthangum: production, recovery, and properties. Biotechnol Adv. 2000; 18:549-579. https://doi.org/10.1016/S0734-9750(00)00050-1
  21. Fatimi A, Tassin JF, Quillard S, Axelos MA, Weiss P. The rheological properties of silated hydroxypropylmethylcellulose tissue engineering matrices. Biomaterials. 2008;29:533-543. https://doi.org/10.1016/j.biomaterials.2007.10.032
  22. Shin-ya Y, Lee M, Hinode H, Kajiuchi T. Effects of Nacetylation degree on N-acetylated chitosan hydrolysis with commercially available and modified pectinases. Biochem Eng J. 2001;7:85-88. https://doi.org/10.1016/S1369-703X(00)00129-7
  23. Becker TA, Kipke DR, Brandon T. Calcium alginate gel: a biocompatible and mechanically stable polymer for endovascular embolization. J Biomed Mater Res. 2001;54:76-86. https://doi.org/10.1002/1097-4636(200101)54:1<76::AID-JBM9>3.0.CO;2-V

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