FLOWER RESEARCH JOURNAL (화훼연구)

The Korean Society for Floricultural Science (한국화훼학회)
권호 표지
DOI QR코드

DOI QR Code

In Vitro Zygotic Embryo Germination and Somatic Embryogenesis through Cotyledonary Explants of Paeonia lactiflora Pall.

작약의 자엽을 이용한 체세포배 형성

Jana, Sonali;Sivanesan, Iyyakkannu;Lim, Mi Young;Jeong, Byoung Ryong
;;임미영;정병룡

  • Published : 20130300
⟨ Previous Next ⟩

Abstract

A simple and efficient protocol was developed for somatic embryogenesis from the cotyledon explant of Paeonia lactiflora Pall. Seeds of peony obtained from fieldgrown plants were disinfested and zygotic embryos were excised. For germination, excised embryos were cultured on the Murashige and Skoog (MS) medium supplemented with 3% (w/v) sucrose, 0.8% (w/v) agar, and different concentrations of $N^6$ benzyl-adenine (BA) and gibberellic acid ($GA_3$). The greatest germination percentage (95%) was observed when embryos were cultured on the MS medium with $1.0mg{\cdot}L^{-1}$ BA and $0.5mg{\cdot}L^{-1}$ $GA_3$, and maintained at $25{\pm}2^{\circ}C$ under a 16 h photoperiod. Thirty days old cotyledon explants were cultured on the MS medium supplemented with different concentrations and combinations of plant growth regulators viz., BA, $GA_3$, 2,4-dichlorophenoxyacetic acid (2,4-D), and ${\acute{a}}$-naphthalene acetic acid (NAA). After 90 days, the globular embryos were directly formed on the surface of explants. The highest frequency of somatic embryo induction (72.5) was obtained on the MS medium with $3.0mg{\cdot}L^{-1}$ BA, $1.0mg{\cdot}L^{-1}$ NAA, $1.0mg{\cdot}L^{-1}$ $GA_3$, and 0.1% (w/v) activated charcoal (AC), with a mean number of 14 embryos per explant. Maturation of globular embryos into heart- and torpedo-shape was observed on the same medium. When the torpedo-shaped embryos were transferred onto the same MS medium supplemented with $3.0mg{\cdot}L^{-1}$ BA, $1.0mg{\cdot}L^{-1}$ NAA, $1.0mg{\cdot}L^{-1}$ $GA_3$, and 0.1% (w/v) AC, secondary somatic embryos were observed on the surface of primary somatic embryos. When the embryos were transferred to the MS medium supplemented with $1.0mg{\cdot}L^{-1}$ each of BA and $GA_3$, all of them converted into plantlets, but their growth was very slow.

Keywords

References

  1. Agarwal S, Kanwar K, Sharma DR (2004) Factors affecting secondary somatic embryogenesis and embryo maturation in Morus alba L. Sci Hort 102:359-368 https://doi.org/10.1016/j.scienta.2004.04.002
  2. Barzilay A, Zemah H, Kamenetsky R (2002) Annual life cycle and floral development of 'Sarah Bernhardt' peony in Israel. Hort Sci 37:300-303
  3. Buchheim JAT, Burkhart LF, Meyer Jr MM (1994) Effect of exogenous gibberellic acid, abscisic acid, and benzylaminopurine on epicotyls dormancy of cultured herbaceous peony embryos. Plant Cell Tiss Organ Cult 36:35-43 https://doi.org/10.1007/BF00048313
  4. Dhekney S, Li Z, Gray D (2011) Factors influencing induction and maintenance of Vitis rotundifolia Michx. embryogenic cultures. Plant Cell Tiss Organ Cult 105:175-180 https://doi.org/10.1007/s11240-010-9849-7
  5. Finkelstein RR (2004) The role of hormones during seed development and germination, pp 513-537. In: Davies PJ (ed) Plant hormones: Biosynthesis, signal transduction, and action. Kluwer Academic, Dordrecht
  6. Gaj MD (2004) Factors influencing somatic embryogenesis induction and plant regeneration with particular reference to Arabidopsis thaliana Heynt. Plant Growth Regul 43:27-47 https://doi.org/10.1023/B:GROW.0000038275.29262.fb
  7. Guleryuz G, Kirmizi S, Arslan H, Sakar FS (2011) Dormancy and germination in Stachys germanica L. subsp. bithynica (Boiss.) Bhattacharjee seeds: Effects of short-time moist chilling and plant growth regulators. Flora: morphology, distribution, functional ecology of plants 206:943-948 https://doi.org/10.1016/j.flora.2011.07.003
  8. Griess JL, Meyer MM (1976) Dormancy and survival of perennial plants. Amer Paeony Soc Bull 231:39-42
  9. Hosoki T, Ando M, Kubara T, Hamada M, Itami M (1989) In vitro propagation of herbaceous peony (Paeonia lactiflora Pall.) by a longitudinal shoot-split method. Plant Cell Rep 8:243-246 https://doi.org/10.1007/BF00778543
  10. Kabar K (1998) Comparative effects of kinetin, benzyladenine, and gibberellic acid on abscisic acid inhibited seed germination and seedling growth of red pine and arbor vitae. Tr J Bot 22:1-6
  11. Kim YS, Lee BK (1996) Somatic embryogenesis and plant regeneration in cotyledon culture of Paeonia albiflora. J Korean Soc Hort Sci 37:827-830
  12. Kim HM, Shin JH, Sohn JK (2006) Cryopreservation of somatic embryos of the herbaceous peony (Paeonia lactiflora Pall.) by air drying. Cryobiology 53:69-74 https://doi.org/10.1016/j.cryobiol.2006.03.012
  13. Kim YJ, Lee OR, Kim KT, Yang DC (2012) High frequency of plant regeneration through cyclic secondary somatic embryogenesis in Panax ginseng. J Ginseng Res 36:442-448 https://doi.org/10.5142/jgr.2012.36.4.442
  14. Koornneef M, Bentsink L, Hilhorst H (2002) Seed dormancy and germination. Curr Opin Plant Biol 5:33-36 https://doi.org/10.1016/S1369-5266(01)00219-9
  15. Lee BK, Ko JA, Kim YS (1992) Studies on the thidiazuron treatment of anther culture in Paeonia albiflora. J Korean Soc Hort Sci 33:384-395
  16. Lee CY, Kim YK, Kim YS, Suh SY, Lee SY, Park SU (2009) Somatic embryogenesis and plant regeneration in Cnidium officinale Makino. J Med Plants Res 3:96-100
  17. Linkies A, Leubner-Metzger G (2012) Beyond gibberllins and abscisic acid: How ethylene and jasmonates control seed germination. Plant Cell Rep 31:253-270 https://doi.org/10.1007/s00299-011-1180-1
  18. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473:497
  19. Rajabpoor S, Azghandi AV, Saboora A (2007) Effects of different concentrations of 2,4-D and BAP on somatic embryogenesis induction in saffron (Crocus sativus L.). Pak J Biol Sci 10:3927-3930 https://doi.org/10.3923/pjbs.2007.3927.3930
  20. Rama Swamy N, Ugandhar T, Parveen M, Venkataiah P, Rambabu M, Upender M, Subhash K (2005) Somatic embryogenesis and plantlet regeneration from cotyledon and leaf explants of Solanum surattense. Indian J Biotechnol 4:414-418
  21. Rogers A (1995) Peonies. p 3 Timber Press, Portland, Oregon, USA.
  22. Sahrawat AK, Chand S (2001) Continuous somatic embryogenesis and plant regeneration from hypocotyls segments of Psoralea corylifolia Linn., an endangered and medicinally important Fabaceae plant. Curr Sci 81:1328-1331
  23. Samson NP, Campa C, Gal LL, Noirot M, Thomas G, Lokeswari TS, Kochko A (2006) Effect of primary culture medium composition on high frequency somatic embryogenesis in different Coffea species. Plant Cell Tiss Organ Cult 86:37-45 https://doi.org/10.1007/s11240-006-9094-2
  24. Shohael AM, Ali MB, Hahn EJ, KY (2007) Glutathione metabolism and antioxidant responses during Eleutherococcus senticocus somatic embryo development in bioreactor. Plant Cell Tiss Organ Cult 89:121-129 https://doi.org/10.1007/s11240-007-9220-9
  25. Sivanesan I, Lim MY, Jeong BR (2011a) Somatic embryogenesis and plant regeneration from leaf and petiole explants of Campanula punctata Lam. Var. rubriflora Makino. Plant Cell Tiss Organ Cult 107:365-369 https://doi.org/10.1007/s11240-011-9983-x
  26. Sivanesan I, Lim MY, Jo EH, Jeong BR (2011b) Somatic embryogenesis and plant regeneration in Crocus vernus. Int. Proc. Chem Biol Environ Eng 4:199-201
  27. Sivanesan I, Son MS, Jana S, Jeong BR (2012) Secondary somatic embryogenesis in Crocus vernus L. Hill. Propag Ornam Plants 12:163-170
  28. Sohn JK, Kwon YS, Shin YA (2002) Effect of phenylacetic acid (PAA) on formation in anther and microspore culture of Paeonia lactiflora. Korean J Plant Biotechnol 29:193-198 https://doi.org/10.5010/JPB.2002.29.3.193
  29. Stanys V, Mazeikiene I, Staniene G, Siksnianas T (2007) Effect of phytohormones and stratification on morphogenesis of Paeonia lactiflora Pall. isolated embryos. Biologija 18:27-30
  30. Thomas TD (2008) The role of activated charcoal in plant tissue culture. Biotechnol Adv 26:618-631 https://doi.org/10.1016/j.biotechadv.2008.08.003
  31. Tian D, Tilt KM, Dane F, Woods FM, Sibley JL (2010) Comparison of shoot induction ability of different explants in herbaceous peony (Paeonia lactiflora Pall.). Sci Hort 123:385-389 https://doi.org/10.1016/j.scienta.2009.10.007
  32. Vengadesan G, Pijut PM (20090 Somatic embryogenesis and plant regeneration of northern red oak (Quercus rubra L.). Plant Cell Tiss Organ Cult 97:141-149 https://doi.org/10.1007/s11240-009-9508-z
  33. Verma SK, Yucesan BB, Gurel S, Gurel E (2011) Indirect somatic embryogenesis and shoot organogenesis from cotyledonary leaf segments of Digitalis lamarckii Ivan., an endemic medicinal species. Turk J Biol 35:743-750
  34. Wu H, Yu X, Teixeira da Silva JA, Lu G (2011) Direct shoot induction of Paeonia lactiflora 'Zhong Sheng Fen' and rejuvenation of hyperhydric shoots. New Zeal J Crop Hort 39:271-278 https://doi.org/10.1080/01140671.2011.594445
  35. Yu X, Wu H, Teixeira da Silva JA, Shen M (2012) Multiple shoot induction and rooting of Paeonia lactiflora 'Da Fu Gui'. Afr J Biotechnol 11:9776-9781