Tissue Engineering and Regenerative Medicine

Korean Tissue Engineering and Regenerative Medicine Society (한국조직공학·재생의학회)
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Fabrication of 3D Alginate Scaffold with Interconnected Pores using Wire-Network Molding Technique

  • Lee, Se-Hwan (Division of Mechanical and Automotive Engineering, College of Engineering, Wonkwang University) ;
  • Jo, A Ra (Division of Mechanical and Automotive Engineering, College of Engineering, Wonkwang University) ;
  • Choi, Ghi Pyoung (Division of Mechanical and Automotive Engineering, College of Engineering, Wonkwang University) ;
  • Woo, Chang Hee (Division of Mechanical and Automotive Engineering, College of Engineering, Wonkwang University) ;
  • Lee, Seung Jae (Division of Mechanical and Automotive Engineering, College of Engineering, Wonkwang University) ;
  • Kim, Beom-Su (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • You, Hyung-Keun (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Cho, Young-Sam (Division of Mechanical and Automotive Engineering, College of Engineering, Wonkwang University)
  • Published : 2013.04.01
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Abstract

In this study, we fabricated 3D porous scaffold by 'Wire-Network Molding' technique with alginate gel which has been used for cartilage regeneration because of the chemical similarity. Firstly, prepared ETPCS-S wires with size of rectangular cross section $600\;{\mu}m$ by $600\;{\mu}m$, $400\;{\mu}m$ by $400\;{\mu}m$, respectively, and the wires are inserted in designed mold. Secondly, sterilized 2 wt% alginate gel within hMSC (human Mesenchymal stem cell) was injected into the assembled mold. The concentration of hMSC in the used alginate gel is about 5000 cells per scaffold. For the gelation of alginate gel, the mold was soaked in 5 wt% $CaCl_2$ solution for 5 min. Subsequently, wires are separated from the mold and the mold is removed from alginate gel. Consequently, the remained alginate scaffold has interconnected pores with a configuration of wire-network. Additionally, to analyze the cell-culturing characteristics, 1-day, 3-day, and 7-day cultured scaffolds which encapsulate hMSC are assessed using MTS assay. Consequently, the optical density of $400\;{\mu}m$-WNM scaffolds and $600\;{\mu}m$-WNM scaffolds are clearly more increased than control scaffolds without pores.

Keywords

References

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