Journal of Preventive Veterinary Medicine

The Korean Society of Preventive Veterinary Medicine (한국예방수의학회)
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Immunostimulatory effects of Bordetella bronchiseptica antigen on mouse spleen cells

  • Jo, Yongchan (College of Veterinary Medicine, Jeju National University) ;
  • Joo, Hong-Gu (College of Veterinary Medicine, Jeju National University)
  • Published : 2013.03.31
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Abstract

Bordetella bronchiseptica (B. bronchiseptica) is a Gram negative bacterium that causes a common respiratory disease in animals including pigs. It is a major etiological pathogen for atropic rhinitis, causing damage to the swine industry. Despite many efforts in development of an efficient vaccine against B. bronchiseptica, studies characterizing antigenic properties of B. bronchiseptica are lacking. In this study, we investigated the intact immunogenicity of B. bronchiseptica in the absence of vaccine adjuvant using mouse spleen cells. A preparation of formalin-treated intact B. bronchiseptica and mouse spleen cells was used for the experimental setup. The activation and viability of spleen cells treated with B. bronchiseptica was measured by MTT assay and Trypan blue exclusion test, respectively. A concentration range of B. bronchiseptica generated some clusters of spleen cells, indicating high proliferation. For functional analysis, production of TNF-alpha was measured using specific ELISA after harvest of culture supernatants and expression of lymphocyte activation markers (CD25, CD69) was analyzed by flow cytometry. By itself, B. bronchiseptica showed significant proliferation and functional enhancement of spleen cells. Taken together, the intact immunogenecity of B. bronchiseptica may affect the development of its vaccine.

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