Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Changes in the Growth and Biochemical Composition of Nannochloropsis sp. Cultures Using Light-Emitting Diodes

LED (Light Emitting Diode)를 이용한 Nannochloropsis sp.의 성장 및 생화학적 조성 변화

  • Park, Jin-Chul (East costal Life Science Institute Gangneung-Wonju National University) ;
  • Kwon, O-Nam (Marine Biology Center for Reserach and Education, Gangneung-Wonju National University) ;
  • Hong, Sung-Eic (East Sea Fisheries Research Institute, National Fisheries Research & Development Institute) ;
  • An, Heui-Chun (East Sea Fisheries Research Institute, National Fisheries Research & Development Institute) ;
  • Bae, Jea-Hyun (East Sea Fisheries Research Institute, National Fisheries Research & Development Institute) ;
  • Park, Mi-Sun (Aquaculture Management Division, National Fisheries Research & Development Institute) ;
  • Park, Heum-Gi (Department of Marine Bioscience, Gangneung-Wonju National University)
  • 박진철 (강릉원주대학교 동해안생명과학연구소) ;
  • 권오남 (강릉원주대학교 해양생물연구교육센터) ;
  • 홍성익 (국립수산과학원 동해수산연구소) ;
  • 안희춘 (국립수산과학원 동해수산연구소) ;
  • 배재현 (국립수산과학원 동해수산연구소) ;
  • 박미선 (국립수산과학원 양식관리과) ;
  • 박흠기 (강릉원주대학교 해양자원육성학과)
  • Received : 2013.03.08
  • Accepted : 2013.06.11
  • Published : 2013.06.30
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Abstract

The marine microalgae Nannochloropsis sp. was cultured under a metal halide lamp (MH) or light-emitting diodes (LEDs) of various wavelengths (blue, LB; red, LR; yellow, LY; green, LG; white, LW); changes in growth, total carotenoid, chlorophyll a, amino acid and fatty acid profiles were investigated. LB-exposed cultures exhibited the highest specific growth rate (SGR) (0.32), whereas LY-exposed cultures showed the lowest SGR (0.18). After cultivation for 9 days, the maximum dry cell weight (g/L) of LB-exposed cultures was significantly higher than that of those exposed to other light conditions (LB>MH>LW$${\geq_-}LG{\geq_-}$$LR>LY). The essential amino acid (EAA, %) contents of cultures exposed to LG, LB, LR and MH were higher than those exposed to LY and LW (P<0.05). Eicosapentaenoic acid and n-3 highly unsaturated fatty acid levels were significantly higher in MH-exposed cultures compared to those exposed to LY (P<0.05), with no marked difference compared to those exposed to LB, LG, LR and LW (P>0.05). The total carotenoid content was highest in LR-exposed cultures (18.0 mg/L), whereas MH showed the lowest (11.8 mg/L; P<0.05). Chlorophyll a content was highest in cultures exposed to LR compared to other light sources. These results suggest consistent differences in growth and biochemical composition after exposure to light of different wavelengths.

Keywords

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