Journal of Pharmacopuncture (대한약침학회지)

KOREAN PHARMACOPUNCTURE INSTITUTE (대한약침학회)
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Study on a Single-Dose Toxicity Test of D-Amino Acid Oxidase (DAAO) Extracts Injected into the Tail Vein of Rats

  • Kang, Jungue (Wonkwang University College of Oriental Medicine) ;
  • Lee, Eun-Yong (Department of Acupuncture & Moxibustion Medicine, Semyung University Oriental Medicine Hospital) ;
  • Song, Bong-Keun (Department of Internal Medicine, Wonkwang Gwangju Oriental Medical Hospital) ;
  • Lee, Seung-Deok (Department of Acupuncture & Moxibustion, Dongguk University College of Oriental Medicine) ;
  • Yook, Tae-Han (Department of Acupuncture & Moxibustion, Woosuk University College of Korean Medicine) ;
  • Ahn, Seong-Hun (Department of Meridian & Acupoint, Wonkwang University College) ;
  • Son, Il-Hong (Department of Neurology, Wonkwang University Sanbon Hospital) ;
  • Kim, Sungchul (Department of Acupuncture & Moxibustion, Wonkwang Gwangju Oriental Medical Hospital)
  • Received : 2013.04.15
  • Accepted : 2013.04.23
  • Published : 2013.06.30
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Abstract

Objective: This study was performed to analyze the single-dose toxicity of D-amino acid oxidase (DAAO) extracts. Methods: All experiments were conducted at the Korea Testing & Research Institute (KTR), an institution authorized to perform non-clinical studies, under the regulations of Good Laboratory Practice (GLP). Sprague-Dawley rats were chosen for the pilot study. Doses of DAAO extracts, 0.1 to 0.3 cc, were administered to the experimental group, and the same doses of normal saline solution were administered to the control group. This study was conducted under the approval of the Institutional Animal Ethics Committee. Results: In all 4 groups, no deaths occurred, and the $LD_{50}$ of DAAO extracts administered by IV was over 0.3 ml/kg. No significant changes in the weight between the control group and the experimental group were observed. To check for abnormalities in organs and tissues, we used microscopy to examine representative histological sections of each specified organ, the results showed no significant differences in any organs or tissues. Conclusion: The above findings suggest that treatment with D-amino acid oxidase extracts is relatively safe. Further studies on this subject should be conducted to yield more concrete evidence.

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References

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