Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
권호 표지
DOI QR코드

DOI QR Code

Skin Permeability of Porcine Placenta Extracts and Its Physiological Activities

  • Han, JeungHi (Department of Food and Nutrition, Korea University) ;
  • Kim, Mi-Ryung (Department of Home Economics, Korea University) ;
  • Park, Yooheon (Department of Food and Nutrition, Korea University) ;
  • Hong, Yang Hee (Department of Food and Nutrition, Korea University) ;
  • Suh, Hyung Joo (Department of Food and Nutrition, Korea University)
  • Received : 2013.04.06
  • Accepted : 2013.06.12
  • Published : 2013.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

We investigated the skin permeability and various biological activities of porcine homogenate of placenta (HP) with the highest protein contents (452.89 ${\mu}g/mg$). The content of protein in subcritical extract of HP (SPE) was decreased from the initial content of 452.9 ${\mu}g/mg$ to 262.7 ${\mu}g/mg$ at 3 h subcritical extract. The contents of amino type nitrogen (A-N) were sharply increased from 35.1 ${\mu}g/mg$ of initial content to 305.9 ${\mu}g/mg$ at 3 h subcritical extract. The HP showed a noticeable activity in terms of antioxidant capacity for ferric reducing antioxidant power (FRAP) assay and especially for 2,2'-Azinobis- (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) method. HP, SPE-0.5, SPE-2 and SPE-3 showed inhibitory effect on elastase activities with an $IC_{50}$ of 46.1, 42.9, 31.6 and 34.7 ${\mu}g/mL$, respectively. SPEs showed more significantly inhibitory effect than HP (p<0.05). The skin permeability of the SPEs was higher than that of the HP. SPE-3 showed highest skin permeation and the permeability was significantly higher than that of HP. SPE-2 also showed significantly higher permeation than HP after 4 h. As expected, increase of extraction time significantly increased skin permeability in the subcritical extract of HP (SPE). From these results, in terms of cost and source availability, porcine placenta extracted with subcritical extraction has advantages over untreated PE and have potential as a cosmetic ingredient.

Keywords

References

  1. Adler-Nissen, J. (1979) Determination of the degree of hydrolysis of food protein hydrolysates by trinitrobenzenesulfonic acid. J. Agric. Food Chem. 27, 1256-1262. https://doi.org/10.1021/jf60226a042
  2. An, B. J., Kwak, J. H., Park, J. M., Lee, J. Y., Park, T. S., Lee, J. T., Son, J. H., Jo, C., and Byun, M. W. (2005) Inhibition of enzyme activities and the antiwrinkle effect of polyphenol isolated from the persimmon leaf (Diospyros kaki folium) on human skin. Dermatol. Surg. 31, 848-854.
  3. Benzie, I. F. F. and Strain, J. (1996) The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal. Biochem. 239, 70-76. https://doi.org/10.1006/abio.1996.0292
  4. Bos, J. D. and Meinardi, M. M. (2000) The 500 Dalton rule for the skin penetration of chemical compounds and drugs. Exp. Dermatol. 9, 165-169. https://doi.org/10.1034/j.1600-0625.2000.009003165.x
  5. Briganti, S., Camera, E., and Picardo, M. (2003) Chemical and instrumental approaches to treat hyperpigmentation. Pigment Cell Res. 16, 101-110. https://doi.org/10.1034/j.1600-0749.2003.00029.x
  6. Do, Y.-K., Kim, J.-M., Chang, S.-M., Hwang, J.-H., and Kim, W.-S. (2009) Enhancement of polyphenol bio-activities by enzyme reaction. J. Mol. Catal. B-Enzym. 56, 173-178. https://doi.org/10.1016/j.molcatb.2008.08.003
  7. Fang, X. P., Xia, W. S., Sheng, Q. H., and Wang, Y. L. (2007) Purification and characterization of an immunomodulatory Peptide from bovine placenta water-soluble extract. Prep. Biochem. Biotechnol. 37, 173-184. https://doi.org/10.1080/10826060701386653
  8. Georgieva, R., Stefanov, D., Fichorova, R., and Dimitrova, E. (1995) Effects of the whole extract and the chromatographic fractions of the pig placenta on lymphocyte proliferation and humoral immune response. Theriogenology 44, 539-551. https://doi.org/10.1016/0093-691X(95)00225-W
  9. Gil-Izquierdo, A., Zafrilla, P., and Tomás-Barberán, F. A. (2002) An in vitro method to simulate phenolic compound release from the food matrix in the gastrointestinal tract. Eur. Food Res. Technol. 214, 155-159. https://doi.org/10.1007/s00217-001-0428-3
  10. Hong, J. W., Lee, W. J., Hahn, S. B., Kim, B. J., and Lew, D. H. (2010) The effect of human placenta extract in a wound healing model. Ann. Plast. Surg. 65, 96-100. https://doi.org/10.1097/SAP.0b013e3181b0bb67
  11. Kim, B. Y., Kim, T., Kang, W. Y., Hyun, B., Cheon, H. Y., and Kim, D. (2010) Functional cosmetic effect of porcine placeta. Korean Chem. Eng. Res. 48, 327-331.
  12. Kim, H. J., Lee, J. W., Kim, Y. I., and Lee, M. H. (2003) The effect of placental extract on the expression of tyrosinase, TRP-1 and TRP-2 in SK30 melanoma cells. Korean J. Dermatol. 41, 1612-1618.
  13. Kim, T., Kim, S., Kang, W., Baek, H., Jeon, H. Y., Kim, B. Y., Kim, C. G., and Kim, D. (2011) Porcine amniotic fluid as possible antiwrinkle cosmetic agent. Korean J. Chem. Eng. 28, 1839-1843. https://doi.org/10.1007/s11814-011-0047-z
  14. Kraunsoe, J. A. E., Claridge, T. D. W., and Lowe, G. (1996) Inhibition of human leukocyte and porcine pancreatic elastase by homologues of bovine pancreatic trypsin inhibitor. Biochemistry 35, 9090-9096. https://doi.org/10.1021/bi953013b
  15. Lee, K. H., Park, H. J., Seo, H. G., Kim, J. H., Lim, G. S., Lee, W. Y., Kim, N. H., Lee, J. H., Jung, H. S., Sung, S. H., and Song, H. (2013) Immune modulation effect of porcine placenta extracts in weaned the pig. J. Anim. Sci. (in press).
  16. Mason, H. S. (1955) Reactions between quinones and proteins. Nature 175, 771 - 772.
  17. McGregor, C. G., Davies, W. R., Oi, K., Teotia, S. S., Schirmer, J. M., Risdahl, J. M., Tazelaar, H. D., Kremers, W. K., Walker, R. C., Byrne, G. W., and Logan, J. S. (2005) Cardiac xenotransplantation: Recent preclinical progress with 3-month median survival. J. Thorac. Cardiovasc. Surg. 130, 844-851.
  18. Mendis, E., Rajapakse, N., and Kim, S. K. (2005) Antioxidant properties of a radical-scavenging peptide purified from enzymatically prepared fish skin gelatin hydrolysate. J. Agric. Food Chem. 53, 581-587. https://doi.org/10.1021/jf048877v
  19. Nath, S. and Bhattacharyya, D. (2007) Cell adhesion by aqueous extract of human placenta used as wound healer. Indian J. Exp. Biol. 45, 732.
  20. Pal, P., Mallick, S., Mandal, S. K., Das, M., Dutta, A. K., Datta, P. K., Bera, R., and Bhadra, R. (2002) A human placental extract: in vivo and in vitro assessments of its melanocyte growth and pigment-inducing activities. Int. J. Dermatol. 41, 760-767. https://doi.org/10.1046/j.1365-4362.2002.01524.x
  21. Pruitt, S. K., Kirk, A. D., Bollinger, R. R., Marsh Jr, H. C., Collins, B. H., Levin, J. L., Mault, J. R., Heinle, J. S., Ibrahim, S., and Rudolph, A. R. (1994) The effect of soluble complement receptor type 1 on hyperacute rejection of porcine xenografts. Transplantation 57, 363. https://doi.org/10.1097/00007890-199402150-00009
  22. Saiga, A., Tanabe, S., and Nishimura, T. (2003) Antioxidant activity of peptides obtained from porcine myofibrillar proteins by protease treatment. J. Agric. Food Chem. 51, 3661- 3667. https://doi.org/10.1021/jf021156g
  23. Sonavane, G., Tomoda, K., Sano, A., Ohshima, H., Terada, H., and Makino, K. (2008) In vitro permeation of gold nanoparticles through rat skin and rat intestine: Effect of particle size. Colloid Surface B 65, 1-10. https://doi.org/10.1016/j.colsurfb.2008.02.013
  24. Tandon, H. and Prakash, A. (1972) Pathology of intestinal tuberculosis and its distinction from Crohn's disease. Gut 13, 260-269. https://doi.org/10.1136/gut.13.4.260
  25. Tiwary, S. K., Shukla, D., Tripathi, A. K., Agrawal, S., Singh, M. K., and Shukla, V. K. (2006) Effect of placental-extract gel and cream on non-healing wounds. J. Wound Care 15, 325-328. https://doi.org/10.12968/jowc.2006.15.7.26937
  26. Togashi, S., Takahashi, N., Iwama, M., Watanabe, S., Tamagawa, K., and Fukui, T. (2002) Antioxidative collagen-derived peptides in human-placenta extract. Placenta 23, 497-502. https://doi.org/10.1053/plac.2002.0833
  27. Tonello, G., Daglio, M., Zaccarelli, N., Sottofattori, E., Mazzei, M., and Balbi, A. (1996) Characterization and quantitation of the active polynucleotide fraction (PDRN) from human placenta, a tissue repair stimulating agent. J. Pharm. Biomed. Anal. 14, 1555-1560. https://doi.org/10.1016/0731-7085(96)01788-8
  28. Wang, L. L. and Xiong, Y. L. (2005) Inhibition of lipid oxidation in cooked beef patties by hydrolyzed potato protein is related to its reducing and radical scavenging ability. J. Agric. Food. Chem. 53, 9186-9192. https://doi.org/10.1021/jf051213g
  29. Wiboonsirikul, J., Hata, S., Tsuno, T., Kimura, Y., and Adachi, S. (2007) Production of functional substances from black rice bran by its treatment in subcritical water. LWT-Food Sci. Technol. 40, 1732-1740. https://doi.org/10.1016/j.lwt.2007.01.003
  30. Wiechers, J. W. (1989) The barrier function of the skin in relation to percutaneous absorption of drugs. Pharm. Weekbl. Sci. 11, 185-198. https://doi.org/10.1007/BF01959410
  31. Wrathall, A. E. (1997) Risks of transmitting scrapie and bovine spongiform encephalopathy by semen and embryos. Rev. Sci. Tech. 16, 240-264.
  32. Zhang, C., Lu, Y., Tao, L., Tao, X., Su, X., and Wei, D. (2007) Tyrosinase inhibitory effects and inhibition mechanisms of nobiletin and hesperidin from citrus peel crude extracts. J. Enzyme Inhib. Med. Chem. 22, 83-90. https://doi.org/10.1080/14756360600953876

Cited by

  1. Effect of Porcine Placenta Extract from Subcritical Water Extraction on Photodamage in Human Keratinocytes vol.35, pp.2, 2015, https://doi.org/10.5851/kosfa.2015.35.2.164
  2. Effects of Ethanol Addition on the Efficiency of Subcritical Water Extraction of Proteins and Amino Acids from Porcine Placenta vol.35, pp.2, 2015, https://doi.org/10.5851/kosfa.2015.35.2.265
  3. Anti-oxidation and Anti-wrinkling Effects of Jeju Horse Leg Bone Hydrolysates vol.34, pp.6, 2014, https://doi.org/10.5851/kosfa.2014.34.6.844
  4. Effect of Porcine Placenta Extract Supplement on Skin Condition in Healthy Adult Women: A Randomized, Double-Blind Placebo-Controlled Study vol.12, pp.6, 2013, https://doi.org/10.3390/nu12061671
  5. A Review on the Extraction and Processing of Natural Source-Derived Proteins through Eco-Innovative Approaches vol.9, pp.9, 2021, https://doi.org/10.3390/pr9091626