Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Antioxidant Capacity and Bioactive Composition of a Single Serving Size of Regular Coffee Varieties Commercially Available in Korea

시판 커피 한 컵에 함유된 생리활성 성분과 항산화활성

  • Kim, Mi-Jeong (Department of Food and Nutrition, Sungshin Women's University) ;
  • Park, Ji-Eun (Department of Food and Nutrition, Sungshin Women's University) ;
  • Lee, Joo-Hyun (Department of Food and Nutrition, Sungshin Women's University) ;
  • Choi, Na-Rae (Department of Food and Nutrition, Sungshin Women's University) ;
  • Hong, Myung-Hee (Department of Food and Nutrition, Sungshin Women's University) ;
  • Pyo, Young-Hee (Department of Food and Nutrition, Sungshin Women's University)
  • 김미정 (성신여자대학교 식품영양학과) ;
  • 박지은 (성신여자대학교 식품영양학과) ;
  • 이주현 (성신여자대학교 식품영양학과) ;
  • 최나래 (성신여자대학교 식품영양학과) ;
  • 홍명희 (성신여자대학교 식품영양학과) ;
  • 표영희 (성신여자대학교 식품영양학과)
  • Received : 2013.03.01
  • Accepted : 2013.04.22
  • Published : 2013.06.30
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Abstract

The major hydrophilic bioactive compounds (chlorogenic acid, caffeine, total phenolics, and flavonoids) and the antioxidant capacity in a single size were evaluated for regular coffee varieties commercially available in Korea. The content of total phenols (63.83-110.12 mg gallic acid equivalents) and flavonoids (35.27-69.27 mg catechin equivalents) were spectrophotometrically determined, and the content of chlorogenic acid (5.17-69.78 mg) and caffeine (74.38-146.32 mg) were determined using HPLC-UV. All varieties of regular coffee studied showed antioxidant potential (88.78-487.52 mg trolox equivalents/serving size), which was conferred by their concentrations of phenolic compounds, caffeine and chlorogenic acid. There were significant (p<0.01) correlations between the total phenolics ($r^2$=0.732) and flavonoids ($r^2$=0.8705) and the antioxidant capacity, suggesting that these components were likely a significant contributor to the antioxidant capacity of commercial regular coffee brews.

시판중인 레귤러커피 한 컵에 함유되어 있는 대표적인 생리활성 성분의 함량과 항산화 활성 등을 측정한 결과는 다음과 같다. 총 페놀함량은 gallic acid 등량 값으로 나타낼 때 63.83-110.12 mg이며, 총 플라보노이드 함량은 catechin 등량 값으로 35.27-69.27 mg이었다. 커피용액 시료에 있는 이들 물질의 분포는 수용성층에 각각 96%와 91% 이상 함유되었다. 레귤러커피 한 컵에 함유된 CGA와 카페인의 함량은 각각 5.17-69.78 mg과 74.38-146.32 mg으로 측정되었다. DPPH와 ABTS 라디컬 소거능 및 환원력 등의 방법으로 평가한 5개 브랜드의 레귤러커피의 항산화 활성은 표준 항산화제인 Trolox 등량 값으로 88.78-487.52 mg의 범위로 나타나 생리활성 성분의 농도에 따라 브랜드 커피의 항산화 활성은 매우 다르게(p<0.05) 나타남을 알 수 있다. 커피음료의 항산화활성은 수용성 항산화물질 특히 플라보노이드(r2=0.8705) 및 폴리페놀 함량($r^2$=0.7732)과 매우 높은 상관도를 보였으며, 그 밖에 카페인($r^2$=0.6194)과 CGA ($r^2$=0.3871)의 함량도 커피의 항산화활성에 기여하는 주요 항산화성분으로 나타났다. 본 실험에 사용된 5종의 브랜드 커피 중 전체적으로 브랜드 B와 C의 커피가 생리활성 성분의 함량과 항산화활성이 가장 높은 것으로 평가되었다(p<0.05).

Keywords

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