Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Construction of Amylolytic Industrial Strains of Saccharomyces cerevisiae for Improved Ethanol Production from Raw Starch

생전분으로부터 에탄올 생산이 증진된 전분 분해성 산업용 Saccharomyces cerevisiae의 개발

  • Im, Young-Kum (Department of Biological Sciences, College of Natural Sciences, Chonnam National University) ;
  • Park, Jin-Yeong (Department of Biological Sciences, College of Natural Sciences, Chonnam National University) ;
  • Lee, Ja-Yeon (Department of Biological Sciences, College of Natural Sciences, Chonnam National University) ;
  • Choi, Seung-Hyun (Department of Biological Sciences, College of Natural Sciences, Chonnam National University) ;
  • Chin, Jong-Eon (Department of Cosmetology, Dongkang College) ;
  • Ko, Hyun-Mi (Department of Microbiology, College of Medicine, Seonam University) ;
  • Kim, Il-Chul (Department of Biological Sciences, College of Natural Sciences, Chonnam National University) ;
  • Bai, Suk (Department of Biological Sciences, College of Natural Sciences, Chonnam National University)
  • 임영금 (전남대학교 자연과학대학 생물학과) ;
  • 박진영 (전남대학교 자연과학대학 생물학과) ;
  • 이자연 (전남대학교 자연과학대학 생물학과) ;
  • 최승현 (전남대학교 자연과학대학 생물학과) ;
  • 진종언 (동강대학 뷰티미용과) ;
  • 고현미 (서남대학교 의과대학 미생물학교실) ;
  • 김일철 (전남대학교 자연과학대학 생물학과) ;
  • 배석 (전남대학교 자연과학대학 생물학과)
  • Received : 2013.06.03
  • Accepted : 2013.06.21
  • Published : 2013.06.30
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Abstract

To contruct amylolytic industrial strains of Saccharomyces cerevisiae which produce ethanol efficiently from raw starch, the Bacillus amyloliquefaciens ${\alpha}$-amylase genes (Amy) or Aspergillus awamori glucoamylase genes (GA1) was separately introduced into the ribosomal DNA loci in the chromosomes of the raw starch fermenting-parental strain (ATCC 9763/$YIp{\delta}AGSA{\delta}$), using double 18S rDNA-integration system. Ethanol production after 3 days of fermentation by the strain that produced ethanol most efficiently from raw starch (ATCC 9763/$YIp{\delta}AGSA{\delta}$/YIpAG2rD) among the transformant strains was 1.5-times higher than that by the parental strain. This new strain generated 9.2% (v/v) ethanol (72 g/L) from 20% (w/v) raw corn starch and consumed 75% of the raw starch content during the same period.

생전분으로부터 에탄올을 효율적으로 생산하는 전분 분해성 산업용 Saccharomyces cerevisiae를 제조하기 위해 생전분을 발효하는 모균주(ATCC 9763/$YIp{\delta}AGSA{\delta}$)의 염색체내 ribosomal DNA loci에 double 18S rDNA-integration 시스템을 이용하여 Bacillus amyloliquefaciens ${\alpha}$-amylase 유전자(Amy) 혹은 Aspergillus awamori glucoamylase 유전자(GA1)를 다중도입시켰다. 얻어진 형질전환 균주들 중 생전분으로부터 가장 효율적으로 에탄올을 생산하는 균주(ATCC 9763/$YIp{\delta}AGSA{\delta}$/YIpAG2rD)의 발효 3일째 에탄올 생산은 모균주에 비해 1.5배 높았다. 이 새로운 균주는 생옥수수 전분이 20% (w/v) 함유된 배지에서 3일간 발효를 통해 에탄올 9.2% (v/v) (72 g/L)를 생산하였고, 생전분 함유량의 75%를 소비하였다.

Keywords

References

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