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Morphological Characteristics of Decomposition and Browning of Oak Sawdust Medium for Ground Bed Cultivation of Lentinula edodes

표고 지면재배용 참나무 톱밥배지의 분해와 갈변의 형태적 특성

  • Koo, Chang-Duck (Department of Forest Science, Chungbuk National University) ;
  • Lee, Seon-Jeong (Department of Environmental Science and Ecological Engineering, Korea University) ;
  • Lee, Hwa-Yong (Department of Forest Science, Chungbuk National University)
  • Received : 2013.01.05
  • Accepted : 2013.06.18
  • Published : 2013.06.30

Abstract

This study investigated the internal and external morphological characteristics of decomposition and browning of oak sawdust medium for ground bed cultivation of Lentinula edodes. Within fifty days after L. edodes inoculation, surface hyphae on the bed browned. In 110 days, the fungal hyphae occupied and decomposed wood fibers, vessels and parenchymatous cells from the inside as white profuse hyphal mass was amorphously dissolving the saw dust particles from the outer surface. Most of the white hyphal bed surface became cleanly brown, however, some colony surface became blackened and slimy with contaminating bacteria, hyphae and spores. The brown layer was ca. 0.34 mm thick with highly dense and white hyphal mass beneath, whereas the blackened layer was ca. 1.17 mm thick with shrunken hyphae and less decomposed sawdust particles beneath. The surface hardness of the brown surface was ca. $0.73kgf/cm^2$, soft and resilient, while that of the blackened was ca. $0.91kgf/cm^2$, hard and nonresilient. By 150 days Lentinula edodes mushrooms fruited only on the brown surface and not on the blackened medium.

본 연구에서는 표고톱밥 지면재배에서 참나무 톱밥 조직의 분해와 배지의 갈변 특성을 내외부 형태적으로 조사하였다. 표고균 접종 50일후에는 갈변이 시작되었다. 접종 110일 후 표고균사는 참나무 톱밥 목부조직내 도관, 목섬유, 유세포의 내외부에서 세포벽을 분해하고, 톱밥 표면에 밀집한 균사는 세포외벽을 부정형으로 분해하였다. 그동안에 흰색인 배지 표면 균사는 대부분 깨끗한 갈색으로 변하였고, 이 갈변층 두께는 약 0.34 mm로 표고균사가 치밀하였고, 그 아래에는 흰색의 활력있는 균사층이 있었다. 반면에 검정색 층은 두께가 약 1.17 mm로 미끈거리는 표면에는 세균과 잡균 균사 및 포자들이 점유하고, 그 아래에는 활력이 쇠퇴한 균사층이 존재하였다. 갈변표면의 경도는 약 $0.73kgf/cm^2$로 검정색 표면의 경도 약 $0.91kgf/cm^2$보다 물렀으나 탄력이 있었다. 표고 자실체는 표고균사 접종 150일 후에 밝은 갈색의 배지 표면에서는 발생하였으나, 검정색 표면층에서는 발생하지 않았다.

Keywords

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