The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Melanogenesis Inhibitory and Antioxidant Activities of Phellinus baumii Methanol Extract

장수진흙버섯 메탄올 추출물의 멜라닌 생성 저해작용

  • Lee, J.S. (Division of life Sciences, Incheon National University) ;
  • Shin, D.B. (Division of life Sciences, Incheon National University) ;
  • Lee, S.M. (Division of life Sciences, Incheon National University) ;
  • Kim, S.H. (Division of life Sciences, Incheon National University) ;
  • Lee, T.S. (Division of life Sciences, Incheon National University) ;
  • Jung, D.C. (Department of Chemistry, Incheon National University)
  • 이재성 (인천대학교 생명과학기술대학 생명과학부) ;
  • 신도빈 (인천대학교 생명과학기술대학 생명과학부) ;
  • 이수민 (인천대학교 생명과학기술대학 생명과학부) ;
  • 김성환 (인천대학교 생명과학기술대학 생명과학부) ;
  • 이태수 (인천대학교 생명과학기술대학 생명과학부) ;
  • 정덕채 (인천대학교 자연과학대학 화학과)
  • Received : 2013.06.05
  • Accepted : 2013.06.18
  • Published : 2013.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Phellinus baumii is a medicinal mushroom used in Asian countries for a long period of time. The purpose of this study was to investigate the skin whitening activities of methanol extracts from fruiting bodies of P. baumii. To evaluate the antioxidant activities of the extract, polyphenol and flavonoid contents, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity and chelating activity on ferrous ions were studied. For assay of skin whitening activities, tyrosinase and DOPA inhibitory activities, and tyrosinase and melanin synthesis inhibitory activities of B16/F10 melanoma cells treated with the methanol extract were investigated. The total polyphenol content of P. baumii methanol extract was 4.19. DPPH scavenging ability of P. baumii methanol extract was 88.26% in $25{\mu}g/mL$ concentration. We tested tyrosinase inhibitory activity and melanin contents in melanoma cells. The tyrosinase activity was inhibited to 65.17% at the concentration of $125{\mu}g/mL$ and melanin synthesis was inhibited to 57.61% at the concentration of $25{\mu}g/mL$. Overall, the experimental results showed that P. baumii methanol extract had inhibitory activities of tyrosinase and melanin synthesis by dose dependent manner in B16/F10 melanoma cells. Strong ultra-violet absorption spectra in the range of 270~370 nm indicated that ethanol extract of P. baumii could protect the skin from UV. Therefore, P. baumii methanol extract might be used for development of skin whitening, anti-UV and skin care agents.

장수진흙버섯 자실체에서 메탄올을 이용해 추출한 물질의 폴리페놀 함량을 측정한 결과 4.2 mg/g으로 확인되었다. 장수진흙버섯 추출물의 B16/F10 melanoma와 NIH3T3 세포주에 대한 독성시험에서 이들 공시세포는 $200{\mu}g/mL$ 농도에서 50% 이상 생존하여 세포독성은 없는 것으로 확인되었다. DPPH 전자공여능 시험에서 장수진흙버섯 추출물의 항산화 효과는 농도 의존적으로 나타났으며 양성대조군인 BHT에 비해 우수하였다. 장수진흙버섯 추출물을 B16/F10 melanoma 세포에 150 mg/mL 처리하여 세포내 티로시나아제의 저해능을 조사한 결과, 양성대조군인 arbutin은 31.2%의 저해능을 보인 반면, 같은 농도의 장수진흙버섯 추출물 처리군의 저해능은 46.0%로 나타나 장수진흙버섯 추출물의 티로시나아제의 저해능이 양성대조군 보다 높았다. 또한 장수진흙버섯 메탄올 추출물을 B16/F10 melanoma 세포에 처리하여 멜라닌 생성량을 조사한 결과 $100{\mu}g/mL$ 농도 처리군의 멜라닌 생성량은 대조군의 31.3%로 같은 농도의 arbutin을 처리한 군의 43.9%에 비해 효과가 높았으며, 투여한 추출물의 농도가 증가함에 따라 멜라닌의 생성량은 농도 의존적으로 감소하였다. 또한 장수진흙버섯 메탄올 추출물을 분광광도계를 이용하여 자외선 영역에서의 흡수 스펙트럼을 조사한 결과 270-370 nm의 자외선 파장에서 흡광도가 높아 자외선 차단효과가 우수하였다. 따라서 장수진흙버섯의 메탄올 추출물은 피부미백이나 자외선차단 기능성 화장품으로 개발의 가능성이 높다고 사료되었다.

Keywords

References

  1. Aburjai, T. and Natsheh, F. M. 2003. Plants used in cosmetics. Phytother. Res. 17:987-1000. https://doi.org/10.1002/ptr.1363
  2. Ahn, B. K., Kim, R., Choi, D. B. and Kim, Y. D. 2011. Effect of Salicornia bigelovii extract on the activities of whitening and anti-wrinkle. Appl. Chem. Eng. 22:56-60.
  3. Blois, M. S. 1958. Antioxidant determination by the use of a stable free radical. Nature. 181:1199-1200. https://doi.org/10.1038/1811199a0
  4. Briganti, S., Camera, E. and Picardo, M. 2003. Chemical instrumental approaches to treat hyperpigmentation. Pig. Cell Res. 16:101-108. https://doi.org/10.1034/j.1600-0749.2003.00029.x
  5. Cho, Y. H., Lee, B. C., Kim, J. H., Kim, J. H., Pyo, H. B., Zhang, Y. H. and Park, H. D. 2005. Effect of Artemisia anomala S. Moore on antioxidant activity and melanogenesis. Kor. J. Pharmacogn. 36:273-277.
  6. Ikekawa, J., Nakamishi, M., Uehara, N., Chihara, G. and Fukuoka, F. 1968. Antitumor action of some basidiomycetes especially Phellinus linteus. Gann. 59:155-157.
  7. Ishihara, Y., Oka, M., Tsunakawa, M., Tomita, K., Hatori, M., Yamamoto, H., Kamei, H., Miyaki, T., Konishi, M. and Oki, T. 1991. Melanostatin, a new melanin synthesis inhibitor. Production, isolation, chemical properties, structure and biological activity. J. Antibiotics. 44:25. https://doi.org/10.7164/antibiotics.44.25
  8. Kang, Y. H., Park, Y. K. and Lee, G. D. 1996. The nitrite scavenging and electron donating ability of phenol compounds. Kor. J. Food Sci. Technol. 28:232-239.
  9. Kim, M. J. and Ryu, M. J. 2012. Inhibition of melanogenesis and anti-UV properties of Reynoutria elliptica. K. J. Aesthet. Cosmotol. 10:961-968.
  10. Kim, Y. J. and Uyama, H. 2005. Tyrosinase inhibitors from natural and synthetic sources: structure, inhibition mechanism and perspective for the future. Cell Mol. Life Sci. 62:1707-1716. https://doi.org/10.1007/s00018-005-5054-y
  11. Ko, J. Y. and Kim, Y. C. 2010. Effectiveness of Scirpi rhizoma ethanol extract on skin whitening using in vitro test. J. Environ. Toxicol. 25:69-77.
  12. Lee, Y. L., Yen, M. and Mau, J. L. 2007. Antioxidant properties of various extracts from Hypsizigus marmoreus. Food Chem. 104:1-9. https://doi.org/10.1016/j.foodchem.2006.10.063
  13. Masuda, T., Yamashita, D., Takeda, Y. and Yonemori, S. 2005. Screening for tyrosinase inhibitors among extracts of seashore plants and identification of potent inhibitors from Garcinia subelliptica. Biosci. Biotechnol. Biochem. 69:197-201. https://doi.org/10.1271/bbb.69.197
  14. Moreno, M. I. N., Isla, M. I., Sampietro, A. R. and Vattuone, M. A. 2000. Comparison of the free radical-scavenging activity of propolis from several region of Argentina. J. Enthnopharm. 71:109-114. https://doi.org/10.1016/S0378-8741(99)00189-0
  15. Mosmann, T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Immunol. Meth. 65:55. https://doi.org/10.1016/0022-1759(83)90303-4
  16. Nam, B. H., Jo, W. S., Choi, Y. J., Lee, J. Y., Kang, E. Y., Jeong, M. H. and Lee, J. D. 2010. Inhibitory effects of melanin secretion on B16 melanoma cell of Cordyceps militaris water extract. Kor. J. Mycol. 38:167-171. https://doi.org/10.4489/KJM.2010.38.2.167
  17. Park, W. H. and Lee, H. D. 2003. Illustrated book of Korean medicinal mushrooms. Kyo-Hak Publishing Co. Ltd.. Seoul.
  18. Pawelk, J. 1978. Melanoma cells in culture. Methods in Enzymol.58:564-568.
  19. Pomerantz, S. H. 1963. Separation, purification and properties of two tyrosinases from hamster melanoma. J. Biol. Chem. 238:2351-2354.
  20. Rocha-Guzmn, N. E., Gonzlez-Laredo, J. A., Conzalez-Laredo, R. F., Reynoso-Camacho, R., Ramos-Gmez, M., Garcia-Gasca, T., Rodrguez-Muoz, M. E., Guzmn-Maldonado, S. H., Medina-Torres, L. and Lujan-Garca, B. A. 2009. Antioxidant activity and genotoxic effect on HeLa cells of phenolic compounds from infusions of Quercus resinosa leaves. Food Chem. 115:1320-1325. https://doi.org/10.1016/j.foodchem.2009.01.050
  21. Shim, S. M., Im, K. H., Kim, J. W., Shim, M. J., Lee, M. W. and Lee, T. S. 2003. Studies on immuno-modulatory and antitumor effects of crude polysaccharides extracted from Paecilomyces sinclairii. Kor. J. Mycol. 31:155-160. https://doi.org/10.4489/KJM.2003.31.3.155
  22. Song, C. H., Ra, K. S., Yang, B. K. and Jeon, Y. J. 1998. Immuno-stimulating activity of Phellinus baumii. Kor. J. Mycol. 26:86-90.
  23. Swain, T., Hillis, W. E. and Ortga, M. 1959. Phenolic constituents of Prunus domestica. I. Quantitative analysis of phenolic constituents. J. Sci. Food Agric. 10:83-88.
  24. Xu, X. M., Jun, J. Y. and Jeong, I. H. 2007. A study on the antioxidant activity of Hae-Songi mushroom (Hypsizigus mamoreus) hot water extract. J. Kor. Soc. Food Sci. Nutr. 36:1351-1357. https://doi.org/10.3746/jkfn.2007.36.11.1351
  25. Yamakoshi, J., Otsuka, F., Sano, A., Tokutake, S., Saito, M., Kikuchi, M. and Kubota, Y. 2003. Lightening effect on ultraviolet induced pigmentation of guinea pig skin by oral administration of a proanthocyanidin-rich extract from grape seeds. Pig. Cell Res. 16:629-638. https://doi.org/10.1046/j.1600-0749.2003.00093.x
  26. Yang, B., Zhao, M. and Jiang, Y. 2008. Optimization of tyrosinase inhibition activity of ultrasonic-extracted polysaccharides from longan fruit pericarp. Food Chem. 110:294-300. https://doi.org/10.1016/j.foodchem.2008.01.067
  27. Yena, G. C., Duhb, P. D. and Tsaia, L. 2002. Antioxidant and pro-oxidant properties of ascorbic acid and gallic acid. Food Chem. 79:307-313. https://doi.org/10.1016/S0308-8146(02)00145-0
  28. Yim, H. S., Chye, F. Y., Ho, S. K. and Ho, W. C. 2009. Phenolic profiles of selected edible wild mushrooms as affected by extraction solvent, time and temperature. As. J. Food Ag-Ind. 2:392-401.
  29. Ying, J. Z., Mao, X. L., Ma, Q. M., Zong, Y. C. and Wen, H. A. 1987. Icones of medicinal fungi from China. Science Press,Beijing.

Cited by

  1. Mushroom Cosmetics: The Present and Future vol.3, pp.3, 2016, https://doi.org/10.3390/cosmetics3030022
  2. Antioxidant and Immunological Activities of Polysaccharide Extracted from Cultured Mycelia of Schizophyllum commune vol.43, pp.9, 2014, https://doi.org/10.3746/jkfn.2014.43.9.1334
  3. Traditional uses, fermentation, phytochemistry and pharmacology of Phellinus linteus : A review vol.113, 2016, https://doi.org/10.1016/j.fitote.2016.06.009