The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
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A Study on the Inhibitory Effect and Mechanism of Lonicera Japonica on Type I Interferon

금은화의 type I interferon 억제효과 및 기전에 관한 연구

  • Kang, Yong-Goo (Dept. of Oriental Obstetrics & Gynecology, College of Oriental Medicine, Won-Kwang University) ;
  • Ryu, Ik-Han (Dept. of Oriental Obstetrics & Gynecology, College of Oriental Medicine, Won-Kwang University) ;
  • Kim, Song-Baek (Dept. of Oriental Obstetrics & Gynecology, College of Oriental Medicine, Won-Kwang University) ;
  • Choi, Chang-Min (Dept. of Oriental Obstetrics & Gynecology, College of Oriental Medicine, Won-Kwang University) ;
  • Seo, Yun-Jung (Dept. of Oriental Obstetrics & Gynecology, College of Oriental Medicine, Won-Kwang University) ;
  • Cho, Han-Baek (Dept. of Oriental Obstetrics & Gynecology, College of Oriental Medicine, Won-Kwang University)
  • 강용구 (원광대학교 한의과대학 한방부인과학 교실) ;
  • 유익한 (원광대학교 한의과대학 한방부인과학 교실) ;
  • 김송백 (원광대학교 한의과대학 한방부인과학 교실) ;
  • 최창민 (원광대학교 한의과대학 한방부인과학 교실) ;
  • 서윤정 (원광대학교 한의과대학 한방부인과학 교실) ;
  • 조한백 (원광대학교 한의과대학 한방부인과학 교실)
  • Received : 2013.04.24
  • Accepted : 2013.05.09
  • Published : 2013.05.31
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Abstract

Objectives: The purpose of this study was to investigate whether Lonicera japonica(LJ) could inhibit LPS-induced type I IFN production. Methods: To evaluate inhibitory effect of LJ on type I IFN, we examined type I IFN, IRF-1, 7 and IL-10 production on LPS-induced macrophages using real time RT-PCR. Next, we observed the interaction of type I IFN, IRF-1, 7 and IL-10 using IL-10 neutralizing antibody. Finally we examined the activation of STAT-1, 3 using western blot. Results: LJ inhibited Type I IFN expression of mRNA and increased IL-10 expression of mRNA. Also LJ inhibited the level of IRF-1, 7 mRNA and the nuclear translocation of IRF-3. Further more, LJ reduced the activation of STAT-1, 3 which are involved in continuous secretion of immune cytokines. Blockade of IL-10 action caused a significant reduction of type I IFN and IRF-1, 7 than LPS-induced LJ pretreatment. Conclusions: LJ inhibits LPS-induced production of type I IFN by IL-10. This study may provide a clinical basis for anti-inflammatory properties of LJ.

Keywords

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