Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Celecoxib on Cycle Kinetics of Gastric Cancer Cells and Protein Expression of Cytochrome C and Caspase-9

  • Wang, Yu-Jie (Department of Gastroenterology, Zhengzhou People's Hospital Affiliated to Southern Medical University) ;
  • Niu, Xiao-Ping (Department of Digestion Medicine, Yijishan Hospital of Wannan Medicine College) ;
  • Yang, Li (Department of Gastroenterology, Zhengzhou People's Hospital Affiliated to Southern Medical University) ;
  • Han, Zhen (Department of Digestion Medicine, Yijishan Hospital of Wannan Medicine College) ;
  • Ma, Ying-Jie (Department of Gastroenterology, Zhengzhou People's Hospital Affiliated to Southern Medical University)
  • Published : 2013.04.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objective: This investigation aimed to determine effects of celecoxib on the cell cycle kinetics of the gastric cancer cell line MGC803 and the mechanisms involved by assessing expression of cytochrome C and caspase-9 at the protein level. Methods: Cell proliferation of MGC803 was determined by MTT assay after treatment with celecoxib. Apoptosis was assessed using fluorescence staining and cell cycle kinetics by flow cytometry. Western blotting was used to detect the expression of caspase-9 protein and of cytochrome C protein in cell cytosol and mitochondria. Results: Celecoxib was able to restrain proliferation and induce apoptosis in a dose- and time-dependent manner, inducing G0/G1 cell cycle arrest, release of cytochrome C into the cytosol, and cleavage of pro-caspase-9 into its active form. Conclusion: Celecoxib can induce apoptosis in MGC803 cells through a mechanism involving cell cycle arrest, mitochondrial cytochrome C release and caspase activation.

Keywords

References

  1. Arber N (2008). Cyclooxygenase-2 inhibitors in colorectal cancer prevention: point. Cancer Epidemiol Biomarkers Prev, 17, 1852-7. https://doi.org/10.1158/1055-9965.EPI-08-0167
  2. Barr H (2011). Gastrointestinal cancer: current screening strategies. Recent Results Cancer Res, 185, 149-57. https://doi.org/10.1007/978-3-642-03503-6_9
  3. Bazuro GE, Torino F, Gasparini G, Capurso L (2008). Chemoprevention in gastrointestinal adenocarcinoma: for few but not for all? Minerva Gastroenterol Dietol, 54, 429-44.
  4. Calviello G, Di Nicuolo F, Piccioni E, et al (2003). gamma-Tocopheryl quinone induces apoptosis in cancer cells via caspase-9 activation and cytochrome c release. Carcinogenesis, 24, 427-33. https://doi.org/10.1093/carcin/24.3.427
  5. Chakraborti AK, Garg SK, Kumar R, Motiwala HF, Jadhavar PS (2010). Progress in COX-2 inhibitors: a journey so far. Curr Med Chem, 17, 1563-93. https://doi.org/10.2174/092986710790979980
  6. Chen WT, Hung WC, Kang WY, et al (2009). Overexpression of cyclooxygenase-2 in urothelial carcinoma in conjunction with tumor-associated-macrophage infiltration, hypoxiainducible factor-1alpha expression, and tumor angiogenesis. APMIS, 117, 176-84. https://doi.org/10.1111/j.1600-0463.2008.00004.x
  7. Clemett D, Goa KL (2000). Celecoxib: a review of its use in osteoarthritis, rheumatoid arthritis and acute pain. Drugs, 59, 957-80. https://doi.org/10.2165/00003495-200059040-00017
  8. Coussens LM, Werb Z (2002). Inflammation and cancer. Nature, 420, 860-7. https://doi.org/10.1038/nature01322
  9. Entezari Heravi R, Hadizadeh F, Sankian M, et al (2011). Novel selective Cox-2 inhibitors induce apoptosis in Caco-2 colorectal carcinoma cell line. Eur J Pharm Sci, 44, 479-86. https://doi.org/10.1016/j.ejps.2011.09.005
  10. Fischer SM, Hawk ET, Lubet RA (2011). Coxibs and other nonsteroidal anti-inflammatory drugs in animal models of cancer chemoprevention. Cancer Prev Res, 4, 1728-35. https://doi.org/10.1158/1940-6207.CAPR-11-0166
  11. Fisher JC, Gander JW, Haley MJ, et al (2011). Inhibition of cyclooxygenase 2 reduces tumor metastasis and inflammatory signaling during blockade of vascular endothelial growth factor. Vasc Cell, 3, 22. https://doi.org/10.1186/2045-824X-3-22
  12. Fujimura T, Ohta T, Oyama K, Miyashita T, Miwa K (2007). Cyclooxygenase-2 (COX-2) in carcinogenesis and selective COX-2 inhibitors for chemoprevention in gastrointestinal cancers. J Gastrointest Cancer, 38, 78-82. https://doi.org/10.1007/s12029-008-9035-x
  13. Ghosh N, Chaki R, Mandal V, Mandal SC (2010). COX-2 as a target for cancer chemotherapy. Pharmacol Rep, 62, 233-44. https://doi.org/10.1016/S1734-1140(10)70262-0
  14. Gu Q, Wang JD, Xia HH, et al (2005). Activation of the caspase-8/Bid and Bax pathways in aspirin-induced apoptosis in gastric cancer. Carcinogenesis, 26, 541-6.
  15. Hsu YL, Kuo YC, Kuo PL, et al (2005). Apoptotic effects of extract from Antrodia camphorata fruiting bodies in human hepatocellular carcinoma cell lines. Cancer Lett, 221, 77-89. https://doi.org/10.1016/j.canlet.2004.08.012
  16. Khan Z, Khan N, Tiwari RP, et al (2011). Biology of Cox-2: an application in cancer therapeutics. Curr Drug Targets, 12, 1082-93. https://doi.org/10.2174/138945011795677764
  17. Lai MY, Huang JA, Liang ZH, et al (2004). Cyclooxygenase-2 expression: a significant prognostic indicator for patients with colorectal cancer. Clin Cancer Res, 10, 8465-71. https://doi.org/10.1158/1078-0432.CCR-04-0653
  18. Li S, Tong Q, Zhang W, et al (2008). Mechanism of growth inhibitory effects of cyclooxygenase-2 inhibitor-NS398 on cancer cells. Cancer Invest, 26, 333-7. https://doi.org/10.1080/07357900701788056
  19. Liu H, Xiao J, Yang Y, et al (2011). COX-2 expression is correlated with VEGF-C, lymphangiogenesis and lymph node metastasis in human cervical cancer. Microvasc Res, 82, 131-40. https://doi.org/10.1016/j.mvr.2011.04.011
  20. Liou JP, Hsu KS, Kuo CC, Chang CY, Chang JY (2007). A novel oral indoline-sulfonamide agent, N-[1-(4-methoxybenzenesulfonyl)-2, 3-dihydro-1H-indol-7-yl] -isonicotinamide (J30), exhibits potent activity against human cancer cells in vitro and in vivo through the disruption of microtubule. J Pharmacol Exp Ther, 323, 398-405. https://doi.org/10.1124/jpet.107.126680
  21. Matthias C, Schuster MT, Zieger S, Harreus U (2006). COX-2 inhibitors celecoxib and rofecoxib prevent oxidative DNA fragmentation. Anticancer Res, 26, 2003-7.
  22. Mehar A, Macanas-Pirard P, Mizokami A, et al (2008). The effects of cyclooxygenase-2 expression in prostate cancer cells: modulation of response to cytotoxic agents. J Pharmacol Exp Ther, 324, 1181-7.
  23. Ogunwobi OO, Liu C (2011). Hepatocyte growth factor upregulation promotes carcinogenesis and epithelialmesenchymal transition in hepatocellular carcinoma via Akt and COX-2 pathways. Clin Exp Metastasis, 28, 721-31. https://doi.org/10.1007/s10585-011-9404-x
  24. Ohno Y, Ohno S, Suzuki N, et al (2005). Role of cyclooxygenase-2 in immunomodulation and prognosis of endometrial carcinoma. Int J Cancer, 114, 696-701. https://doi.org/10.1002/ijc.20777
  25. Park SW, Kim HS, Hah JW, et al (2010). Celecoxib inhibits cell proliferation through the activation of ERK and p38 MAPK in head and neck squamous cell carcinoma cell lines. Anticancer Drugs, 21, 823-30. https://doi.org/10.1097/CAD.0b013e32833dada8
  26. Pietenpol JA, Stewart ZA (2002). Cell cycle checkpoint signaling: cell cycle arrest versus apoptosis. Toxicology, 181-182, 475-81. https://doi.org/10.1016/S0300-483X(02)00460-2
  27. Smith WL, DeWitt DL, Garavito RM (2000). Cyclooxygenases: structural, cellular, and molecular biology. Annu Rev Biochem, 69, 145-82. https://doi.org/10.1146/annurev.biochem.69.1.145
  28. Sobolewski C, Cerella C, Dicato M, Diederich M (2011). Cox-2 inhibitors induce early c-Myc downregulation and lead to expression of differentiation markers in leukemia cells. Cell Cycle, 10, 2978-93. https://doi.org/10.4161/cc.10.17.16460
  29. Thiel A, Mrena J, Ristimaki A (2011). Cyclooxygenase-2 and gastric cancer. Cancer Metastasis Rev, 30, 387-95. https://doi.org/10.1007/s10555-011-9312-1
  30. Wang GY, Zhang JW, Lu QH, Xu RZ, Dong QH (2007). Berbamine induces apoptosis in human hepatoma cell line SMMC7721 by loss in mitochondrial transmembrane potential and caspase activation. J Zhejiang Univ Sci B, 8, 248-55. https://doi.org/10.1631/jzus.2007.B0248
  31. Wu K, Nie Y, Guo C, et al (2009). Molecular basis of therapeutic approaches to gastric cancer. J Gastroenterol Hepatol, 24, 37-41. https://doi.org/10.1111/j.1440-1746.2008.05753.x
  32. Xiao H, Zhang Q, Lin Y, Reddy BS, Yang CS (2008). Combination of atorvastatin and celecoxib synergistically induces cell cycle arrest and apoptosis in colon cancer cells. Int J Cancer, 122, 2115-24. https://doi.org/10.1002/ijc.23315
  33. Zhang CL, Wu LJ, Tashiro S, Onodera S, Ikejima T (2004). Oridonin induced A375-S2 cell apoptosis via bax-regulated caspase pathway activation, dependent on the cytochrome c/caspase-9 apoptosome. J Asian Nat Prod Res, 6, 127-38. https://doi.org/10.1080/1028602031000147375

Cited by

  1. Curcumin Inhibits Human Non-small Cell Lung Cancer A549 Cell Proliferation Through Regulation of Bcl-2/Bax and Cytochrome C vol.14, pp.8, 2013, https://doi.org/10.7314/APJCP.2013.14.8.4599
  2. Antiproliferative Effects of Celecoxib in Hep-2 Cells through Telomerase Inhibition and Induction of Apoptosis vol.15, pp.12, 2014, https://doi.org/10.7314/APJCP.2014.15.12.4919
  3. Silibinin Inhibits Proliferation, Induces Apoptosis and Causes Cell Cycle Arrest in Human Gastric Cancer MGC803 Cells Via STAT3 Pathway Inhibition vol.15, pp.16, 2014, https://doi.org/10.7314/APJCP.2014.15.16.6791
  4. Rice Bran Phytic Acid Induced Apoptosis Through Regulation of Bcl-2/Bax and p53 Genes in HepG2 Human Hepatocellular Carcinoma Cells vol.15, pp.8, 2014, https://doi.org/10.7314/APJCP.2014.15.8.3731
  5. Potential chemoprotective effects of green propolis, L-lysine and celecoxib on bone marrow cells and peripheral blood lymphocytes of Wistar rats subjected to bladder chemical carcinogenesis vol.29, pp.7, 2014, https://doi.org/10.1590/S0102-86502014000700003
  6. Pathway Analysis for Drug Repositioning Based on Public Database Mining vol.54, pp.2, 2014, https://doi.org/10.1021/ci4005354
  7. Celecoxib, a COX-2 Selective Inhibitor, Induces Cell Cycle Arrest at the G2/M Phase in HeLa Cervical Cancer Cells vol.17, pp.4, 2016, https://doi.org/10.7314/APJCP.2016.17.4.1655
  8. Celecoxib exhibits an anti-gastric cancer effect by targeting focal adhesion and leukocyte transendothelial migration-associated genes vol.12, pp.4, 2016, https://doi.org/10.3892/ol.2016.4976
  9. Carvacrol and human health: A comprehensive review vol.32, pp.9, 2018, https://doi.org/10.1002/ptr.6103