DOI QR코드

DOI QR Code

The Effect of Phaseoli Semen Herbal-acupuncture at $KI_{10}$ in Lipopolysaccharide Induced Acute Nephritis in Rats

음곡에 시행한 적소두약침이 LPS로 유도된 흰쥐의 급성 신장염에 미치는 영향

  • Kwak, Kyu In (Department of Acupuncture & Moxibustion Medicine, College of Oriental Medicine, Daejeon University) ;
  • Kang, Jae Hui (Department of Acupuncture & Moxibustion Medicine, College of Oriental Medicine, Daejeon University) ;
  • Lee, Hyun (Department of Acupuncture & Moxibustion Medicine, College of Oriental Medicine, Daejeon University)
  • 곽규인 (대전대학교 한의과대학 침구의학교실) ;
  • 강재희 (대전대학교 한의과대학 침구의학교실) ;
  • 이현 (대전대학교 한의과대학 침구의학교실)
  • Received : 2013.05.09
  • Accepted : 2013.06.03
  • Published : 2013.06.20

Abstract

Objectives : This study was designed to evaluate the effects of Phaseoli Semen Herbal-acupuncture(PS-HA) at $KI_{10}$ in acute nephritis induced by lipopolysaccharide(LPS) in rat. Methods : The rats were divided into 5 groups, which were control, LPS, PS-HA, NP and saline group. LPS, PS-HA, NP and saline groups were given LPS to induce acute nephritis and control group did not receive LPS. LPS group did not receive any treatment after the onset of acute nephritis. PS-HA, NP and saline group received PS-HA, normal acupuncture, and saline injection at $KI_{10}$ three times per week, respectively. To evaluate the effect of PS-HA at $KI_{10}$, the complete blood count, BUN, creatinine, TNF-${\alpha}$, and CINC-1 in serum were measured. To show its effect on renal function, creatinine, and total protein in urine was measured as well as urine output. The level of myeloperoxidase in renal tissue was quantified and complete histology was done in kidney samples obtained from the rats. Results : PS-HA group showed a significant reduction in the proportion of WBC and neutrophil, serum BUN, TNF-${\alpha}$, and CINC-1 compared to LPS group. Furthermore, a significant increase in urine output and a decrease in urinary creatinine level, MPO in renal tissue, and number of neutrophils at glomerulus was observed in PS-HA group compared to LPS group Conclusions : PS-HA at $KI_{10}$ was shown to have a significantly effect on treating LPS induced acute nephrits. Therefore, future study is needed to further evaluate the clinical usefulness of PS-HA at $KI_{10}$ in treating acute nephritis.

Keywords

References

  1. E Braunwakd, A Fauci, D Kasper, S Hauser, D Longo, J Jameson. Harrison's Principles of Internal Medicine, 15/e. Seoul : MIP. 2003 : 1583-89.
  2. Du HK. Oriental Kidney System Internal Medicine. Seoul : Sungbosa. 2003 : 225-301.
  3. Choi KH. Steps to Internal Medicine(5). Seoul : Jungdam. 2008 : 116-45.
  4. Committee of Korean Textbook Publisher. Herbology. Seoul : Yeonglimsa. 2005 : 355-56.
  5. Ahn DK. Illustrated Book of Korean Medicinal Herbs. Seoul : Kyohaksa. 2002 : 393.
  6. Yim YK, Kim JP, Kim TH. Korean Meridian & Acupoint(Kakron). Daejeon : Obikihoek. 2006 : 676-8.
  7. Committee of Korean Acupuncture & Moxibustion Textbook Publisher. Acupuncture & Moxibustion(Sang). Paju : Jipmundang. 2008 : 175-6.
  8. The Korean Society of Nephrology Restration Board. Renal Replacement Therapy in Korea - Insan Memorial Dialysis Registry 2004-. The Korean Journal of Nephrology. 2005 ; 24(2) : 425-57.
  9. National Health Insurance Service. 2008, Benefits by Frequency of Disease(2004).
  10. Choi JB, Lee KH, Kim YS. Beneficial Effect of Liuwei Dihuang Tang Jia Renshen ․ Huangqi Extract on Cisplatin Induced Kidney Toxicity in Rats. Journal of Korean Oriental Internal Medicine. 2003 ; 24(3) : 579-87.
  11. Hwang YG, Jeong JC. Effect of Salviae Radix on renal tubular reabsorption in rabbits with mercury-induced acute renal failure. Journal of Korean Oriental Internal Medicine. 2000 ; 21(2) : 227-34.
  12. Yoon CH, Jeong JC, Shin US. Effects of Cervus elaphus Acua-Acupuncture's solution on damage of Rat's Kidney induced by Ischemia and Reflow. Journal of Korean Oriental Internal Medicine. 1999 ; 20(1) : 111-21.
  13. Kim KM, Lee H, Kang HJ, Lee YH, Yim YK. Anti-inflammatory Effect of Dianthi Semen Herbal-acupuncture at KI10 on nephritis in rats. Journal of Meridian & Acupoint. 2009 ; 26(1) : 61-77.
  14. Park BM, Hur B, Yim YK. Anti-inflammatory Effect of Plantaginis Semen Herbal-acupuncture at KI10 on LPS-induced nephritis in rats. Journal of Meridian & Acupoint. 2009 ; 26(2) : 127-43.
  15. Lee JH, Kim JH, Kim YI. Anti-inflammatory Effect of Benincasae Semen Herbal-acupuncture at KI10 on nephritis in rats. Journal of Pharmacopuncture. 2010 ; 13(2) : 51-65. https://doi.org/10.3831/KPI.2010.13.1.051
  16. Yoon KS, Kang JH, Lee H. Anti-inflammatory Effect on Desmodii Herba Pharmacopuncture at KI10 on Nephritis in Rats. The Journal of Korean Acupuncture & Moxibustion Society. 2011 ; 28(3) : 43-54.
  17. Cho E, Kang JH, Lee H. Anti-inflammatory Effect of Akebiae Lignum Parmacopuncture at KI10 on LPS-induced Acute Nephritis in Rats. The Journal of Korean Acupuncture & Moxibustion Society. 2012 ; 29(3) : 41-53.
  18. Cho KH, Shin SS, Lee ES, Byun BH, Byun SE, Seo BI. The Effects of Phaseoli Angularis Semen and Artemisiae Iwayomogi Herba on Biochemical and Histological Changes of Rats Fed High Fat Diet. The 35th International Symposium of Korean Society of Life Science. 2002 ; 35 : 65.
  19. Lee WT, Lee JE, Park SH, Lee KL, Park KA, Hong SG. Hole's human anatomy & physiology. Seoul : Jungdam. 2004 : 821-3.
  20. 27 Medical Professors Joint Translation. Textbook of Medical Physiology. Seoul : Jungdam. 2002 : 318-33.
  21. Committee of Korean Renal Internal Medicine Textbook Publisher. Renal Internal Medicine. Seoul : Koonjachulpansa. 2011 : 40-45.
  22. Lee MH. Immunology. Seoul : Shinilbooks. 2008 :
  23. Lee SY, Jeong YS, Kwon OH, Song KS. Clinical Laboratory Medicine 7th ed. Seoul : Yonsei University Press. 2000 : 234-7, 617-9.
  24. Anzai M, Maezawai R, Ohara T, Kodama K, Fukuda T, Kurasawa K. Systemic lupus erythematosus associated with facial edema. overproduction of interleukin-5. and eosinophilia. J Clin Rheumatol. 2008 ; 14(6) : 361-2. https://doi.org/10.1097/RHU.0b013e31818f3b15
  25. Trowbridge HO, Emling RC. Inflammation: a review of the process. 5th Ed. Chicago : Quintessence Pub Co. 1997 : 219-45.
  26. Cho HY, Noh KH, Cho MK et al. Anti-oxidative and Anti-inflammatory Effects of Genistein in BALB/c Mice Injected with LPS. Journal of Korean food science and nutrition. 2008 ; 37(9) : 1126-35.
  27. Park SJ, Song HJ. Anti-inflammatory effect of extract of Pulsatilla koreana NAKAI. in LPSstimulated Murine peritoneal macrophage. Korean Journal of Herbology. 2007 ; 22(1) : 111-7.
  28. Bae GS, Park HJ, Kim DY et al. Inhibitory Effect of Extract of Teucrium veronicoides on the Production of Inflammatory Cytokines. Korean Journal of Herbology. 2008 ; 23(3) : 119-25.
  29. Beutler B and Cerami A. The biology of cachetin TNR-R primary mediator of the host response. Annu ReV Immunol. 1989 ; 7 : 625-55. https://doi.org/10.1146/annurev.iy.07.040189.003205
  30. Abboud HE. Growth factors in glomerulonephritis. Kidney Int. 1993 ; 43(1) : 252-67. https://doi.org/10.1038/ki.1993.39
  31. Yi KN, Kwon OH. Clinical Pathology File(Third Edition). Seoul : Eui-Hak Publishing & Printing Co. 2000 : 95-8, 102-5, 1436-39.
  32. Ha JY, Kim YK, Lee KS, Min KR, Kim YS. Inhibitory effects of herbal extracts on CINC-1 induction in LPS-stimulated rat kidney epitheloid NRK-52E cells. Natural Product Science. 1997 : 59-70.
  33. Song KY, Ji JG, Ham EG. Core Pathology. Seoul : Korea Medical Books Pub. 1998 : 573-620.
  34. Kim SB, Park JS, Seo JY, Kwon GB, Lee HS, Ryu DG. Effects of Semen Phaseoli Water Extract on the Renal Fuction in Rats. Journal of Oriental Physiology. 1999 ; 14(1) : 123-8.
  35. Sela S, Shurtz-Swirski R, Cohen-Mazor M et al. Primed peripheral polymorphonuclear leukocyte: a culprit underlying chronic low-grade inflammation and systemic oxidative stress in chronic kidney disease. Journal of the American Society of Nephrology. 2011 ; 30(7) : 603-15.

Cited by

  1. 음곡에 시술한 비해약침이 Lipopolysaccharide로 유도된 흰쥐의 신장염에 미치는 영향 vol.31, pp.2, 2013, https://doi.org/10.13045/acupunct.2014023
  2. 음곡(陰谷) 백복령(白茯苓)약침이 Lipopolysaccharide로 유도된 흰쥐의 염증성 신(腎) 손상에 미치는 영향 vol.31, pp.2, 2013, https://doi.org/10.13045/acupunct.2014025
  3. 음곡에 시술한 석위약침이 Lipopolysaccharide로 유도된 흰쥐의 신장염에 미치는 영향 vol.32, pp.3, 2013, https://doi.org/10.13045/acupunct.2015035