Mycobiology

The Korean Society of Mycology (한국균학회)
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Diversity and Seasonal Variation of Endophytic Fungi Isolated from Three Conifers in Mt. Taehwa, Korea

  • Kim, Chang-Kyun (Department of Biology Education, Korea National University of Education) ;
  • Eo, Ju-Kyeong (Department of Biology Education, Korea National University of Education) ;
  • Eom, Ahn-Heum (Department of Biology Education, Korea National University of Education)
  • Received : 2013.03.08
  • Accepted : 2013.05.30
  • Published : 2013.06.30
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Abstract

The needled leaves of three conifer species were collected in Mt. Taehwa during different seasons of the year. Total 59 isolates and 19 species of endophytic fungi were isolated from the leaves and identified using morphological and molecular characteristics. As a result, Shannon index was different in its host plant; Larix kaempferi had a highest value of species diversity. According to the sampling season, 9 species of 19 species were isolated during fall season. The results suggest that the existing of host plant and sampling season are major factors of distribution of endophytic fungi.

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References

  1. Kong WS. Species composition and distribution of Korean alpine plants. J Korean Geogr Soc 2002;37:357-70.
  2. Koo KA, Park WK, Kong WS. Dendrochronological analysis of Abies koreana W. at Mt. Halla, Korea: effects of climate change on the growths. Korean J Ecol 2001;24:281-8.
  3. Carroll G. Fungal endophytes in stems and leaves: from latent pathogen to mutualistic symbiont. Ecology 1988;69:2-9. https://doi.org/10.2307/1943154
  4. Arnold AE, Henk DA, Eells RL, Lutzoni F, Vilgalys R. Diversity and phylogenetic affinities of foliar fungal endophytes in loblolly pine inferred by culturing and environmental PCR. Mycologia 2007;99:185-206. https://doi.org/10.3852/mycologia.99.2.185
  5. Kim CK, Eo JK, Eom AH. Diversity of foilar endophytic fungi isolated from Lindera obtusiloba in Korea. Kor J Mycol 2012;40:136-40. https://doi.org/10.4489/KJM.2012.40.3.136
  6. Paul NC, Yu SH. Two species of endophytic Cladosporium in pine trees in Korea. Mycobiology 2008;36:211-6. https://doi.org/10.4489/MYCO.2008.36.4.211
  7. Kil YJ, Eo JK, Eom AH. Molecular identification and diversity of endophytic fungi isolated from Pinus densiflora in Boeun, Korea. Kor J Mycol 2009;37:130-3. https://doi.org/10.4489/KJM.2009.37.2.130
  8. Seo ST, Kim KH, Kim MJ, Hong JS, Park JH, Shin SC. Diversity of fungal endophytes from Pinus koraiensis leaves in Korea. Kor J Mycol 2009;37:108-10. https://doi.org/10.4489/KJM.2009.37.1.108
  9. Arnold AE, Maynard Z, Gilbert GS. Fungal endophytes in dicotyledonous neotropical trees: patterns of abundance and diversity. Mycol Res 2001;105:1502-7. https://doi.org/10.1017/S0953756201004956
  10. White TJ, Bruns T, Lee S, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR protocols: a guide to methods and applications. New York: Academic Press; 1990. p. 315-22.
  11. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731-9. https://doi.org/10.1093/molbev/msr121
  12. Arnold AE. Understanding the diversity of foliar endophytic fungi: progress, challenges, and frontiers. Fungal Biol Rev 2007;21:51-66. https://doi.org/10.1016/j.fbr.2007.05.003
  13. Magurran AE. Ecological diversity and its measurement. Princeton: Princeton University Press; 1988.
  14. Schmit JP, Mueller GM. An estimate of the lower limit of global fungal diversity. Biodivers Conserv 2007;16:99-111. https://doi.org/10.1007/s10531-006-9129-3
  15. Schulz B, Boyle C. The endophytic continuum. Mycol Res 2005;109:661-86. https://doi.org/10.1017/S095375620500273X
  16. Li HY, Shen M, Zhou ZP, Li T, Wei Y, Lin L. Diversity and cold adaptation of endophytic fungi from five dominant plant species collected from the Baima Snow Mountain, Southwest China. Fungal Divers 2012;54:79-86. https://doi.org/10.1007/s13225-012-0153-1