Journal of Sasang Constitutional Medicine (사상체질의학회지)

The Society Of Sasang Constitutional Medicine (사상체질의학회)
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Autophagy inducing Effect of modified Yeoldahanso-tang and its related Proteins in SH-SY5Y cells

열다한소탕(熱多寒少湯) 가감방(加減方)의 자가탐식(自家貪食) 유도 활성과 관련 단백질 탐색

  • Kim, Hee-Ju (Natural Medicine Center, Korea Institute of Science and Technology) ;
  • Bae, Na-Young (Dept. of Sasang Constitution Medicine, Pusan National University School of Korean Medicine) ;
  • Jang, Moon-Hee (Dept. of Sasang Constitution Medicine, College of Korean Medicine, Daejeon Univ.) ;
  • Yang, Hyun-Ok (Natural Medicine Center, Korea Institute of Science and Technology) ;
  • Ahn, Taek-Won (Dept. of Sasang Constitution Medicine, College of Korean Medicine, Daejeon Univ.)
  • 김희주 (한국과학기술연구원 강릉분원 천연의약센터) ;
  • 배나영 (부산대학교 한의전원 사상체질과) ;
  • 장문희 (대전대학교 한의과대학 사상체질과) ;
  • 양현옥 (한국과학기술연구원 강릉분원 천연의약센터) ;
  • 안택원 (대전대학교 한의과대학 사상체질과)
  • Received : 2013.07.01
  • Accepted : 2013.07.29
  • Published : 2013.09.30
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Abstract

Objectives Modified Yeolda-Hanso tang (MYH) is a traditional herbal formula in Korea for various diseases. MYH is containing the 10 herbs : Pueraria lobata (Willd.) Ohwi, Angelica tenuissima Nakai, Scutellaria baicalensis Georgi, Platycodon grandiflorum (Jacq), Angelicae Dahurica, Cimicifuga heracleifolia Kom, Raphanus sativa L., Polygala tenuifolia (Willd), Acorus gramineus Soland and Dimocarpus longan Lour. The 10 herbs is constituted as a ratio of the 6:4:2:1:2:2:2:4:6:6. We investigated neuroprotective effects of MYH on human neuroblastoma SH-SY5Y cells and evaluated the ability of MYH to prevent and treat for neurodegenerative diseases such as Parkinson's disease via basal autophagy enhancement. Methods Pharmacological induction of Autophagy by MYH in SH-SY5Y cells: Induction of autophagy by MYH in human neuroblastoma SH-SY5Y cells was carreid out by immunoblot analysis with several autophagy markers. SH-SY5Y cells were treated with MYH at the concentration of 400 and $800{\mu}g/ml$ for 24 hr. Specifically, the autophagosome proteins LC3 II and Atg5 levels were increased and autophagy pathway related proteins such as beclin-1, PI3 Kinase class III protein, ULK1, mTOR and AMPK were activated. Conclusions MYH can enhance the induction of autophagy through key regulator AMPK, mTOR, and Beclin-1 and it should be considered as a possible candidate of neuroprotective agents for such as Parkinson's disease.

Keywords

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