Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Development of RT-PCR and Nested PCR for Detecting Four Quarantine Plant Viruses Belonging to Nepovirus

RT-PCR과 nested PCR을 이용한 Nepovirus속 식물검역 바이러스 4종의 정밀진단

  • Lee, Siwon (Plant Quarantine Technology Center, Animal and Plant Quarantine Agency) ;
  • Kang, Eun-Ha (Plant Quarantine Technology Center, Animal and Plant Quarantine Agency) ;
  • Shin, Yong-Gil (Plant Quarantine Technology Center, Animal and Plant Quarantine Agency) ;
  • Lee, Su-Heon (School of Applied Biosciences, Kyungpook National University)
  • 이시원 (농림축산검역본부 식물검역기술개발센터) ;
  • 강은하 (농림축산검역본부 식물검역기술개발센터) ;
  • 신용길 (농림축산검역본부 식물검역기술개발센터) ;
  • 이수헌 (경북대학교 응용생명과학부)
  • Received : 2013.03.25
  • Accepted : 2013.09.17
  • Published : 2013.09.30
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Abstract

For quarantine purpose, we developed the RT- and nested PCR module of Tomato black ring virus (TBRV), Arabis mosaic virus (ArMV), Cherry leafroll virus (CLRV) and Grapevine fanleaf virus (GFLV). The PCR modules, developed in this study make diagnosis more convenient and speedy because of same PCR condition. And also, the methods are more accurate because it can check whether the result is contamination or not using the mutation-positive control. We discard or return the 27 cases of Nepovirus infection seed by employing the module past 3 years. This study provides a rapid and useful method for detection of four quarantine plant viruses.

본 연구에서는 식물검역바이러스 4종(TBRV, ArMV, CLRV 및 GFLV)을 RT-PCR과 nested PCR 방법으로 진단 할 수 있는 방법을 개발하였다. 본 연구에서 개발한 방법은 모두 같은 PCR 조건으로 검사자에게 편리성과 신속성을 높여줄 뿐 아니라, 돌연변이-양성대조구의 사용으로 실험 오염여부를 확인할 수 있어 더욱 정확하다. 개발한 방법으로 최근 3년 Nepovirus속 4종의 바이러스를 검사한 결과, 27건을 검출하여 검역처분 하였다. 본 연구 결과들은 앞으로도 수출입 식물에서 해당 바이러스들을 신속, 정밀하게 진단할 수 있는 방법으로 활용할 수 있을 것으로 기대된다.

Keywords

References

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