Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Biocontrol of Pectobacterium carotovorum subsp. carotovorum Using Bacteriophage PP1

  • Lim, Jeong-A (Division of Microbial Safety, National Academy of Agricultural Science, Rural Development Administration) ;
  • Jee, Samnyu (Highland Agriculture Research Center, National Institute of Crop Science, Rural Development Administration) ;
  • Lee, Dong Hwan (Division of Microbial Safety, National Academy of Agricultural Science, Rural Development Administration) ;
  • Roh, Eunjung (Division of Microbial Safety, National Academy of Agricultural Science, Rural Development Administration) ;
  • Jung, Kyusuk (Division of Microbial Safety, National Academy of Agricultural Science, Rural Development Administration) ;
  • Oh, Changsik (Department of Horticultural Biotechnology and Institute of Life Science and Resources, Kyung Hee University) ;
  • Heu, Sunggi (Division of Microbial Safety, National Academy of Agricultural Science, Rural Development Administration)
  • Received : 2013.04.02
  • Accepted : 2013.05.08
  • Published : 2013.08.28
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Abstract

Pectobacterium carotovorum subsp. carotovorum (formerly Erwinia carotovora subsp. carotovora) is a plant pathogen that causes soft rot and stem rot diseases in several crops, including Chinese cabbage, potato, and tomato. To control this bacterium, we isolated a bacteriophage, PP1, with lytic activity against P. carotovorum subsp. carotovorum. Transmission electron microscopy revealed that the PP1 phage belongs to the Podoviridae family of the order Caudovirales, which exhibit icosahedral heads and short non-contractile tails. PP1 phage showed high specificity for P. carotovorum subsp. carotovorum, and several bacteria belonging to different species and phyla were resistant to PP1. This phage showed rapid and strong lytic activity against its host bacteria in liquid medium and was stable over a broad range of pH values. Disease caused by P. carotovorum subsp. carotovorum was significantly reduced by PP1 treatment. Overall, PP1 bacteriophage effectively controls P. carotovorum subsp. carotovorum.

Keywords

References

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