Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Effects of the Inclusion of Kimchi Lactic Acid Bacterial Culture in Extruded Pellets on the Growth, Body Composition and Immune Response of Juvenile Olive Flounder Paralichthys olivaceus

김치 유산균 배양물의 부상용 배합사료내 첨가시 넙치(Paralichthys olivaceus) 치어의 성장, 체조성 및 면역성 반응에 미치는 효과

  • Jeon, Gyu Ho (Division of Marine Environment and BioScience, Korea Maritime University) ;
  • Cho, Sung Hwoan (Division of Marine Environment and BioScience, Korea Maritime University) ;
  • Kim, Hee Sung (Division of Marine Environment and BioScience, Korea Maritime University) ;
  • Myung, Sung Hyo (Division of Marine Environment and BioScience, Korea Maritime University) ;
  • Kim, Hyeon Jong (Division of Marine Environment and BioScience, Korea Maritime University) ;
  • Jung, Won-Gwan (Division of Marine Environment and BioScience, Korea Maritime University) ;
  • Park, Byum Hee (MyungSun Co. LTD.) ;
  • Lee, Keum Joo (MyungSun Co. LTD.)
  • 전규호 (한국해양대학교 해양환경생명과학부) ;
  • 조성환 (한국해양대학교 해양환경생명과학부) ;
  • 김희성 (한국해양대학교 해양환경생명과학부) ;
  • 명성효 (한국해양대학교 해양환경생명과학부) ;
  • 김현종 (한국해양대학교 해양환경생명과학부) ;
  • 정원관 (한국해양대학교 해양환경생명과학부) ;
  • 박범희 (명선해양산업(주)) ;
  • 이금주 (명선해양산업(주))
  • Received : 2013.06.12
  • Accepted : 2013.09.05
  • Published : 2013.10.31
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Abstract

This study was performed to determine effects of the supplementation of Kimchi lactic acid bacterial culture in extruded pellets (EP) on the growth, body composition, blood chemistry and immune response of olive flounder Paralichthys olivaceus. Four hundred eighty individuals averaging 16.1 g were randomly distributed into 12, 180 L flow-through tanks (forty fish per tank). Four concentrations of Kimchi lactic acid bacterial culture (KL) were prepared: Control (0%), 0.1%, 0.2% and 0.5%. Three concentrations (0.1%, 0.2% and 0.5%) of Kimchi lactic acid bacterial culture were each diluted to 10% of EP weight and then fully absorbed by EP for 10 minutes. Each diet was fed to triplicate groups of fish. Fish were hand-fed to apparent satiation twice a day for 8 weeks. At the end of the 8-week feeding trial, the plasma lysozyme and bacterial activities of fish were determined. In addition, the cumulative mortality of fish was monitored for 8 days after their artificial infection with Edwardsiella tarda. The weight gain, specific growth rate, feed efficiency ratio, protein efficiency ratio, protein retention, hepatosomatic index and condition factor of fish were not affected by dietary supplementation with KL. None of the proximate composition, plasma parameters, lysozyme or bactericidal activities of fish was affected by dietary supplementation with KL. However, the cumulative mortalities of fish fed EP containing 0.1% and 0.5% Kimchi lactic acid bacterial culture were relatively low compared to that of fish fed the control diet. In conclusion, dietary supplementation with KL did not effectively improve growth, feed utilization, body composition, plasma chemistry, lysozyme, bactericidal activities or immune response of olive flounder after E. tarda infection under these experimental conditions.

Keywords

References

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