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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Antioxidant Activity of a Chitin-degrading Bacterium Bacillus idriensis (CGH18)

키틴분해 박테리아 Bacillus idriensis (CGH18)의 항산화 활성

  • Jung, Myoung Eun (Division of Marine Enviroment & Bioscince, College of Ocean Science and Technology, Korea Marine University) ;
  • Hong, Joo Wan (Division of Marine Enviroment & Bioscince, College of Ocean Science and Technology, Korea Marine University) ;
  • Lee, Jeong-Im (Division of Marine Enviroment & Bioscince, College of Ocean Science and Technology, Korea Marine University) ;
  • Kwak, Myoung Kuk (Division of Marine Enviroment & Bioscince, College of Ocean Science and Technology, Korea Marine University) ;
  • Kim, Hojun (Division of Marine Enviroment & Bioscince, College of Ocean Science and Technology, Korea Marine University) ;
  • Sohn, Jae Hak (Department of Bio-food material, College of Medical & Life Sciences, Silla University) ;
  • Song, Young-Sun (Center of Smart Foods and Drugs and Food Science Institute, Inje University) ;
  • Oh, Kwang-Suk (Department of Architecture and Ocean Space, College of Ocean Science and Technology, Korea Marine University) ;
  • Seo, Youngwan (Division of Marine Enviroment & Bioscince, College of Ocean Science and Technology, Korea Marine University)
  • 정명은 (한국해양대학교 해양과학기술대학 해양환경.생명과학부) ;
  • 홍주완 (한국해양대학교 해양과학기술대학 해양환경.생명과학부) ;
  • 이정임 (한국해양대학교 해양과학기술대학 해양환경.생명과학부) ;
  • 곽명국 (한국해양대학교 해양과학기술대학 해양환경.생명과학부) ;
  • 김호준 (한국해양대학교 해양과학기술대학 해양환경.생명과학부) ;
  • 손재학 (신라대학교 의생명과학대학 바이오식품소재학과) ;
  • 송영선 (인제대학교 의생명공학대학 식의약생명공학과) ;
  • 오광석 (한국해양대학교 해양과학기술대학 해양공간건축학과) ;
  • 서영완 (한국해양대학교 해양과학기술대학 해양환경.생명과학부)
  • Received : 2013.06.18
  • Accepted : 2013.07.18
  • Published : 2013.08.30
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Abstract

A bacterium CGH18 exhibiting antioxidizing and chitin-degrading activities in the colloidal chitin culture medium was isolated from salt-fermented crab. This strain was identified as Bacillus idriensis based on 16S rDNA sequence homology search. Its crude extract was partitioned between n-BuOH and $H_2O$. The organic layer was further partitioned between $CH_2$ $Cl_2$ and $H_2O$. Antioxidant activities of crude extract and its solvent fractions were evaluated using five different bioassay methods, including the degree of occurrence of intracellular reactive oxygen species (ROS), peroxynitrite scavenging (ONOO), and oxidative damage of genomic DNA. All fractions exhibited significant antioxidant activity in bioassay systems used.

Keywords

References

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