KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Diagnostic Paper Chip for Reliable Quantitative Detection of Albumin using Retention Factor

체류 인자를 이용한, 알부민의 정량 분석용 종이 칩

  • Jeong, Seong-Geun (Department of Chemical Engineering, Chungnam National University) ;
  • Lee, Sang-Ho (Micro/Nano Scale Manufacturing Research Group, Korea Institute of Industrial Technology) ;
  • Lee, Chang-Soo (Department of Chemical Engineering, Chungnam National University)
  • 정성근 (충남대학교 공과대학 화학공학과) ;
  • 이상호 (한국생산기술연구원 마이크로나노공정연구그룹) ;
  • 이창수 (충남대학교 공과대학 화학공학과)
  • Received : 2013.06.28
  • Accepted : 2013.08.25
  • Published : 2013.08.30
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Abstract

Herein we present a diagnostic paper chip that can quantitatively detect albumin without external electronic reader and dispensing apparatus. We fabricated a diagnostic paper chip device by printing wax barrier on the paper and wicking it with citrate buffer and tetrabromophenol blue to detect albumin in sample solution. The paper chip is so simple that we dropped a sample solution at sample pad and measure the ratio of two travel distances of the sample solvent and albumin under the name of retention factor. Our result confirmed that the retention factor was constant in the samples with same concentration of albumin and useful determinant for the measurement of albumin concentration. The paper chip is affordable and equipment-free, and close to ideal point-of-care test in accordance with the assured criteria, outlined by the World Health Organization. We assume that this diagnostic paper chip will expand the concept of colorimetric determination and provide a inexpensive diagnostic method to aging society and developing country.

Keywords

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