Sterilization Effects on Mulberries (Morus alba L.) Washed with Electrolyzed Water and Chlorine Dioxide

전해수와 이산화염소수 세척에 따른 뽕나무 오디(Morus alba L.)의 살균효과

  • Teng, Hui (School of Food Science & Biotechnology, Kyungpook National University) ;
  • Lee, Sun-Ho (Post-Harvest Engineering, National Academy of Agricultural Science, Rural Development Administrations) ;
  • Lee, Won-Young (School of Food Science & Biotechnology, Kyungpook National University)
  • 텅후이 (경북대학교 식품공학부) ;
  • 이선호 (농촌진흥청 국립농업과학원 농업공학부) ;
  • 이원영 (경북대학교 식품공학부)
  • Received : 2013.07.03
  • Accepted : 2013.10.30
  • Published : 2013.10.31

Abstract

The current research is designed to analyze sterilization effects on mulberries in terms of storage temperature and storage period after treating with tap water (TW), electrolyzed water (EW) and aqueous chlorine dioxide ($ClO_2$). The treated $ClO_2$ concentrations are 10, 50, 100 and 200 ppm. In each concentration, the mulberries are soaked for 30 seconds respectively. The sterilization effects are being compared at room temperature ($25^{\circ}C$) and at $4^{\circ}C$, respectively. And the enzyme activity related to quality is also being investigated and analyzed about for browning inhibition effects. Microbial sterilizing power increases more in treating plots with EW and $ClO_2$ than treating plot with TW. Futhermore sterilizing power of $ClO_2$ increased sharply on high concentration treatment plot as well. Sterilization effects of $ClO_2$ during storage time are better at cold temperature. Pictures taken from scanning electron microscope reveal that there are no microbes in sterilizing solutions treatment plots. From measurement of the enzyme activity, it is concluded that activities decrease more in sterilizing solutions treatment plots as comparing with TW treated plot during the time. The amount of total polyphenolics decrease with the time passing and EW and $ClO_2$ treatment shows less contents than TW treatment. Thus, EW and $ClO_2$ treatment of mulberris are considered as method to improve safety by reducing total plate count and to contribute to quality maintenance and to extend storage time.

본 연구에서는 수도수, 전해수, 이산화염소수 처리에 의한 오디의 저장온도 및 기간에 따른 품질 양상을 분석하였다. 오디를 수도수, 전해수와 이산화염소수 농도별 10, 50, 100, 200 ppm으로 각각 30초 동안 담금질을 한 후, 실온과 냉장에 저장하여 시간에 따른 저장성 효과를 비교하였다. 또한, 외관 품질 유지와 갈변 저해 효과를 탐색하였다. 또한, 오디 과실 표면으로부터 Enterobacter aerogenes와 Serratia marcescens의 위해미생물을 분리 동정하였다. 살균세척수를 처리한 후, 저장 온도별 시간에 따른 미생물 총 균수를 측정한 결과, 각 처리구의 미생물 살균력은 전해수와 이산화염소수가 수도수 처리보다 증가하였으며, 이산화염소수는 고농도일수록 현저하게 증가하였다. 상온에서 저장하는 처리구보다 냉장 저장한 처리구에서의 미생물 억제효과가 뛰어났다. 오디의 표면에 주사전자현미경 촬영 결과, 살균 세척수 처리구에서는 미생물이 생존하지 않았다. 효소활성을 측정한 결과, 수도수 처리구보다 살균 세척수 처리구에서 함량이 낮아졌으며, 시간이 지남에 따라 서서히 증가하였다. 총 폴리페놀 함량은 세척 방법 간 유사한 결과를 나타내었다. 저장일수가 증가할수록 감소하였고, 전해수와 이산화염소수 처리구의 함량이 수도수 처리보다는 낮았다. 따라서 오디의 전해수와 이산화염소수 처리는 초기의 미생물 총 균수를 감소시켜 신선도를 유지하고, 상온보다 냉장 저장에서 효과가 더 우수하였으며, 품질 유지 및 저장성 향상에 기여할 것으로 사료된다.

Keywords

References

  1. Abadias M, Usall J, Alegre I, Vinas I (2008) Efficacy of neutral electrolyzed water (NEW) for reducing microbial contamination on minimally-processed vegetables. Int J Food Microbiol 123: 151-158. https://doi.org/10.1016/j.ijfoodmicro.2007.12.008
  2. Aebi H (1974) Catalase. in: Methods of Enzymatic Analysis. Academic Press, New York. pp 673-684.
  3. Chen Z, Zhu C (2011) Combined effects of aqueous chlorine dioxide and ultrasonic treatments on postharvest storage quality of plum fruit (Prunus salicina L.). Postharv Biol Technol 61: 117-123. https://doi.org/10.1016/j.postharvbio.2011.03.006
  4. Havsteen B (1983) Flavonoid, a class of natural products of high pharmacological potency. Biochem Pharmacol 32: 1141-1148. https://doi.org/10.1016/0006-2952(83)90262-9
  5. Hejazi A, Falkiner FR (1997) Serratia marcescens. J Med Microbiol 46: 903-912. https://doi.org/10.1099/00222615-46-11-903
  6. Heo SI, Jin YS, Jung MJ, Wang MH (2007) Antidiabetic properties of 2,5-dihydroxy-4,3'-di(${\beta}$-D-glucopyranosyloxy) -trans-stilbene from mulberry (Morus bombycis Koidzumi) root in streptozotocin-induced diabetic rats. J Med Food 10: 602-607. https://doi.org/10.1089/jmf.2006.0241
  7. Jeong JW, Kim JH, Kwon KH, Park KJ (2006) Disinfection effects of electrolyzed water on strawberry and quality changes during storage. Korean J Food Preserv 13: 316-321.
  8. John G (1997) Essentials of Food Microbiology. 1st ed. Arnold Publishers, London. pp 97-98.
  9. Jung SW, Park KJ, Park KJ, Park BI, Kim YH (1996) Surface sterilization effect of electrolyzed acid water on vegetable. Korean J Food Sci Technol 28: 1045-1051.
  10. Kar M, Mishra D (1976) Catalase, peroxidase and polyphenoloxidase activities during rice leaf senescence. Plant Physiol 57: 315-319. https://doi.org/10.1104/pp.57.2.315
  11. Kim HB, Ryu KS (2000) Sensory characteristics of mulberry fruit jam and wine. Korean J Seric Sci 42: 73-77.
  12. Kim HB, Kim SY, Ryu KS, Lee WC, Moon JY (2001) Effect of methanol extract from mulberry fruit on the lipid metabolism and liver function in cholesterol-induced hyperlipidemia rats. Korean J Seri Sci 43: 104-108.
  13. Kim MW, Kim AJ (2007) The quality characteristics of mulberry fruit wine by two different manufacturing methods. Korean J Food & Nutr 20: 276-281.
  14. Kim SK (1991) Beneficial medicine, mulberry fruit. In Bonchohak. Younglimsa, Seoul. pp 598-605.
  15. Kim YG, Kim TW, Ding T, Oh DH (2009) Effect of electrolyzed water and citric acid on quality enhancement and microbial inhibition in head lettuce. Korean J Food Sci Technol 41: 578-586.
  16. Koseki S, Yosida K, Kamitani Y, Itoh K (2004) Efficacy of acidic electrolyzed water for microbial decontamination of cucumbers and strawberries. J Food Protect 66: 1247-1251.
  17. Kumazawa S, Hamasaka T, Nakayama T (2004) Antioxidant activity of propolis of various geographic origins. Food Chem 84: 329-339. https://doi.org/10.1016/S0308-8146(03)00216-4
  18. Lee HH, Hong SI, Kim DM (2009) Microbiological characterization and chlorine treatment of buckwheat sprouts. Korean J Food Sci Technol 41: 452-457.
  19. Lee WJ, Lee CH, Yoo JY, Kim KY, Jang KI (2011) Sterilization efficacy of washing method using based on microbubbles and electrolyzed water on various vegetables. J Korean Soc Food Sci Nut 40: 912-917. https://doi.org/10.3746/jkfn.2011.40.6.912
  20. Liu M, Qian B, Zhang H, Deng Y, Shen Y, Ping J, Cao L (2010) Sanitizer treatments alleviate lignification of sliced few-flower wildrice (Zizania latifolia Turcz.). Food Res Int 43: 2363-2368. https://doi.org/10.1016/j.foodres.2010.09.004
  21. Park CM, Hung YC, Doyle MP, Ezeke GOI, Kim C (2001) Pathogen reduction and quality of lettuce treated with electrolyzed water. J Food Sci 66: 1368-1372. https://doi.org/10.1111/j.1365-2621.2001.tb15216.x
  22. Park KJ, Lim JH, Kim JH, Jeong JW, Jo JH, Jeong SW (2007) Reduction of microbial load on radish (Raphanus sativus L.) seeds by aqueous chlorine dioxide and hot water treatment. Korean J Food Preserv 14: 487-491.
  23. Park KJ, Lim JH, Kim BK, Kim JC, Jeong JW, Jeong SW (2008) Effect of aqueous chlorine dioxide and citric acid on reduction of Salmonella typhimurium on sprouting radish seeds. Korean J Food Preserv 15: 754-759.
  24. Park SS, Sung JM, Jeong JW, Park KJ, Lim JH (2012) Efficacy of electrolyzed water and aqueous chlorine dioxide for reducing pathogenic microorganism on chinese cabbage. Korean J Food Sci Technol 44: 240-246. https://doi.org/10.9721/KJFST.2012.44.2.240
  25. Ryu SH (2007) Effects of aqueous chlorine dioxide against Escherichia coli O157:H7 and Listeria monocytogenes on broccoli served in food service institutions. J Korean Soc Food Sci Nutr 36: 1622-1627. https://doi.org/10.3746/jkfn.2007.36.12.1622
  26. Sanders Jr WE, Sanders CC (1997) Enterobacter spp.: pathogens poised to flourish at the turn of the century. Clin Microbiol Rev 10: 220-241.
  27. Shigenobu K, Seiichiro I (2007) Microbial control of fresh produce using electrolyzed water. Jpn Agr Res 41: 273-282. https://doi.org/10.6090/jarq.41.273
  28. Song HJ, Chun HH, Jo WS, Song KB (2012) Effects of aqueous chlorine dioxide and UV-C irradiation on decontamination and growth of microbes during chilled storage of celery and cherries. J Korean Soc Food Sci Nutr 41: 402-407. https://doi.org/10.3746/jkfn.2012.41.3.402
  29. Sung JM, Park KJ, Lim JH, Jeong JW (2012) Removal effects of microorganism and pesticide residues on Chinese cabbages by electrolyzed water washing. Korean J Food Sci Technol 44: 628-633. https://doi.org/10.9721/KJFST.2012.44.5.628
  30. Vandekinderen I, Camp JV, Meulenaer BD, Veramme K, Bernaert N, Denon Q, Ragaert P, Devlieghere F (2009) Moderate and high doses of sodium hypochlorite, neutral electrolyzed oxidizing water, peroxyacetic acid, and gaseous chlorine dioxide did not affect the nutritional and sensory qualities of fresh-cut iceberg lettuce (Lactuca sativa var. capitata L.) after washing. J Agric Food Chem 57: 4195-4203. https://doi.org/10.1021/jf803742v
  31. Yoo JY, Jang KI (2011) Changes in quality of soybean sprouts washed with electrolyzed water during storage. J Korean Soc Food Sci Nutr 40: 586-592. https://doi.org/10.3746/jkfn.2011.40.4.586