Tuberculosis and Respiratory Diseases

The Korean Academy of Tuberculosis and Respiratory Diseases (대한결핵및호흡기학회)
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Effects of Lobetyolin, Lobetyol and Methyl linoleate on Secretion, Production and Gene Expression of MUC5AC Mucin from Airway Epithelial Cells

  • Yoon, Yong Pill (Department of Pharmacology, Chungnam National University School of Medicine) ;
  • Ryu, Jiho (Department of Pharmacology, Chungnam National University School of Medicine) ;
  • Park, Su Hyun (Department of Pharmacology, Chungnam National University School of Medicine) ;
  • Lee, Hyun Jae (Department of Pharmacology, Chungnam National University School of Medicine) ;
  • Lee, Seungho (Department of Pharmacy, College of Pharmacy, Yeungnam University) ;
  • Lee, Sang Kook (Department of Pharmacy, College of Pharmacy, Seoul National University) ;
  • Kim, Ju-Ock (Pulmonology Section, Department of Internal Medicine, Chungnam National University Hospital) ;
  • Hong, Jang-Hee (Department of Pharmacology, Chungnam National University School of Medicine) ;
  • Seok, Jeong Ho (Department of Pharmacology, Chungnam National University School of Medicine) ;
  • Lee, Choong Jae (Department of Pharmacology, Chungnam National University School of Medicine)
  • Received : 2014.06.01
  • Accepted : 2014.08.21
  • Published : 2014.11.28
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Abstract

Background: In this study, we investigated whether lobetyolin, lobetyol, and methyl linoleate derived from Codonopsis pilosula affect MUC5AC mucin secretion, production, and gene expression from airway epithelial cells. Methods: Confluent NCI-H292 cells were pretreated with lobetyolin, lobetyol, or methyl linoleate for 30 minutes and then stimulated with phorbol 12-myristate 13-acetate (PMA) for 24 hours. The MUC5AC mucin gene expression, and mucin protein production and secretion were measured by reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. Results: Lobetyolin, lobetyol, and methyl linoleate inhibited the gene expression of MUC5AC mucin induced by PMA; lobetyolin did not affect PMA-induced MUC5AC mucin production. However, lobetyol and methyl linoleate inhibited the production of MUC5AC mucin; lobetyolin and lobetyol did not significantly affect PMA-induced MUC5AC mucin secretion from NCI-H292 cells. However, methyl linoleate decreased the MUC5AC mucin secretion. Conclusion: These results suggest that among the three compounds, methyl linoleate can regulate gene expression, production, and secretion of MUC5AC mucin by directly acting on the airway epithelial cells.

Keywords

References

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