Journal of The Korean Society of Grassland and Forage Science (한국초지조사료학회지)

The Korean Society of Grassland and Forage Science (한국초지조사료학회)
권호 표지
DOI QR코드

DOI QR Code

Proteomic Response of Alfalfa Subjected to Aluminum (Al) Stress at Low pH Soil

  • Rahman, Md. Atikur (Division of Applied Life Sciences (BK21Plus), IALS, PMBBRC, Gyeongsang National University) ;
  • Kim, Yong-Goo (Division of Applied Life Sciences (BK21Plus), IALS, PMBBRC, Gyeongsang National University) ;
  • Lee, Byung-Hyun (Division of Applied Life Sciences (BK21Plus), IALS, PMBBRC, Gyeongsang National University)
  • Received : 2014.12.16
  • Accepted : 2014.12.31
  • Published : 2014.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

In order to reveal the aluminum (Al) stress tolerance mechanisms in alfalfa plant at low pH soil, a proteomic approach has been conducted. Alfalfa plants were exposed to Al stress for 5 days. The plant growth and total chlorophyll content are greatly affected by Al stress. The malondialdehyde (MDA) and $H_2O_2$ contents were increased in a low amount but free proline and soluble sugar contents, and the DPPH-radical scavenging activity were highly increased. These results indicate that antioxidant activity (DPPH activity) and osmoprotectants (proline and sugar) may involve in ROS ($H_2O_2$) homeostasis under Al stress. In proteomic analysis, over 500 protein spots were detected by 2-dimentional gel electrophoresis analysis. Total 17 Al stress-induced proteins were identified, of which 8 protein spots were up-regulated and 9 were down-regulated. The differential expression patterns of protein spots were selected and analyzed by the peptide mass fingerprinting (PMF) using MALDI-TOF MS analysis. Three protein spots corresponding to Rubisco were significantly down-regulated whereas peroxiredoxin and glutamine synthetase were up-regulated in response to Al stress. The different regulation patterns of identified proteins were involved in energy metabolism and antioxidant / ROS detoxification during Al stress in alfalfa. Taken together, these results provide new insight to understand the molecular mechanisms of alfalfa plant in terms of Al stress tolerance.

Keywords

References

  1. Bates, L.S., Waldren, R.P. and Teare, I.D. 1973. Rapid determination of free proline for water-stress studies. Plant and Soil. 39: 205-207. https://doi.org/10.1007/BF00018060
  2. Duressa, D., Soliman, K., Taylor, R. and Senwo, Z. 2011. Proteomic analysis of soybean roots under aluminum stress. International Journal of Plant Genomics. 2011:1-12.
  3. Dwivedi, R.S., Breiman, A. and Herman, E.M. 2003. Differential distribution of the cognate and heat-stress-induced isoforms of high Mr cis-trans prolyl peptidyl isomerase (FKBP) in the cytoplasm and nucleoplasm. Journal of Experimental Botany 54:2679-2689. https://doi.org/10.1093/jxb/erg307
  4. Ezzine, M. and Ghorbel, M.H. 2006. Physiological and biochemical responses resulting from nitrite accumulation in tomato (Lycopersicon esculentum Mill. cv. Ibiza F1). Journal of Plant Physiology. 163:1032-1039. https://doi.org/10.1016/j.jplph.2005.07.013
  5. Fukui, K., Wakamatsu, T., Agari, Y., Masui, R. and Kuramitsu, S. 2011. Inactivation of the DNA repair genes mutS, mutL or the anti-recombination gene mutS2 leads to activation of vitamin B1 biosynthesis genes. PLoS One 6:e19053. https://doi.org/10.1371/journal.pone.0019053
  6. Haider, S.I., Kang, W., Ghulam, J. and Guo-ping, Z. 2007. Interactions of cadmium and aluminum toxicity in their effect on growth and physiological parameters in soybean. Journal of Zhejiang University Science B. 8:181-188. https://doi.org/10.1631/jzus.2007.B0181
  7. Hansen, J. and Moller, I.B. 1975. Percolation of starch and soluble carbohydrates from plant tissue for quantitative determination with anthrone. Anal Biochemistry : 87-94.
  8. Houde, M. and Diallo, A.O. 2008. Identification of genes and pathways associated with aluminum stress and tolerance using transcriptome profiling of wheat near-isogenic lines. BMC Genomics. 9:1-13. https://doi.org/10.1186/1471-2164-9-1
  9. Hurkman, W.J. and Tanaka, C.K. 1986. Solubilization of plant membrane proteins for analysis by two dimensional gel electrophoresis. Plant Physiology. 81:802-806. https://doi.org/10.1104/pp.81.3.802
  10. Kang, H.-M. and Saltveit, M.E. 2001. Antioxidant Enzymes and DPPH-radical scavenging activity in chilled and heat-shocked rice (Oryza sativa L.) seedlings radicles. Journal of Agricultural and Food Chemistry. 50:513-518.
  11. Kochian, L.V., Hoekenga, O.A. and Pineros, M.A. 2004. How do crop plants tolerate acid soils? Mechanisms of aluminum tolerance and phosphorous efficiency. Annual Review of Plant Biology. 55:459-493. https://doi.org/10.1146/annurev.arplant.55.031903.141655
  12. Lee, D.G., Ahsan, N., Lee, S.H., Kang, K.Y., Bahk, J.D., Lee, I.J. and Lee, B.H. 2007. A proteomic approach in analyzing heatresponsive proteins in rice leaves. Proteomics. 7:369-3383.
  13. Lichtenthaler, H.K. 1987. Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes. In: Lester Packer R.D (ed) Methods in Enzymology, vol. Volume 148, Academic Press, pp. 350-382.
  14. Lin, C.C. and Kao, C.H. 2001. Abscisic acid induced changes in cell wall peroxidase activity and hydrogen peroxide level in roots of rice seedlings. Plant Science. 160: 323-329. https://doi.org/10.1016/S0168-9452(00)00396-4
  15. Liu, P., Yang, Y.S., Xu, G., Guo, S., Zheng, X. and Wang, M. 2006. Physiological responses of four herbaceous plants to aluminum stress in South China. Frontiers of Biology in China 1:295-302. https://doi.org/10.1007/s11515-006-0037-4
  16. Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. 1951. Protein measurement with the folinphenol reagent. Journal of Biological Chemistry. 193:265-275.
  17. Manzano-Leon, N., Delgado-Coello, B., Guaderrama-Diaz, M. and Mas-Oliva, J. 2006. ${\beta}$-adaptin: Key molecule for microglial scavenger receptor function under oxidative stress. Biochemical and Biophysical Research Communications. 351:588-594. https://doi.org/10.1016/j.bbrc.2006.10.077
  18. Narasimhamoorthy, B., Blancaflor, E.B., Bouton, J.H., Payton, M.E. and Sledge, M.K. 2007. A comparison of hydroponics, soil, and root staining methods for evaluation of aluminum tolerance in Medicago truncatula (Barrel Medic) germplasm. Crop Science. 47: 321-328. https://doi.org/10.2135/cropsci2006.03.0147
  19. Nunes-Nesi, A., Brito, D.S., Inostroza-Blancheteau, C., Fernie, A.R. and Araujo W.L. 2014. The complex role of mitochondrial metabolism in plant aluminum resistance. Trends in Plant Science. 19:399-407. https://doi.org/10.1016/j.tplants.2013.12.006
  20. Pietrini, F., Iannelli, M.A., Pasqualini. S. and Massacci, A. 2003. Interaction of cadmium with glutathione and photosynthesis in developing leaves and chloroplasts of Phragmites australis (Cav.) Trin. ex Steudel. Plant physiology. 133:829-837. https://doi.org/10.1104/pp.103.026518
  21. Sha Valli Khan, P.S., Nagamallaiah, G.V., Dhanunjay Rao, M., Sergeant, K. and Hausman, J.F. 2014. Abiotic stress tolerance in plants Insights from proteomics. In: Emerging Technologies and Management of Crop Stress Tolerance A Sustainable Approach, Academic Press, USA, 2: pp.41-45.
  22. Sharma, A.D. and Singh, P. 2003. Comparative studies on droughtinduced changes in peptidyl prolyl cis-trans isomerase activity in drought-tolerant and susceptible cultivars of Sorghum bicolor. Current Science. 84:911-918.
  23. Vidigal, P., Carvalho, R., Amancio, S. and Carvalho, L. 2013. Peroxiredoxins are involved in two independent signalling pathways in the abiotic stress protection in Vitis vinifera. Biologia Plantarum. 57:675-683. https://doi.org/10.1007/s10535-013-0346-9
  24. Weger, H.G. and Turpin, D.H. 1989. Mitochondrial respiration can support NO(3) and NO(2) reduction during photosynthesis : Interactions between photosynthesis, respiration, and N assimilation in the N-limited green alga Selenastrum minutum. Plant Physiology. 89: 409-415. https://doi.org/10.1104/pp.89.2.409
  25. Wilkins, M.R., Sanchez, J.C., Gooley, A.A., Appel, R.D., Humphery-Smith, I., Hochstrasser, D.F. and Williams, K.L. 1996. Progress with proteome projects: why all proteins expressed by a genome should be identified and how to do it. Biotechnology and Genetic Engineering Reviews. 13:19-50. https://doi.org/10.1080/02648725.1996.10647923
  26. Zhang, Y., Xu, W., Li, Z., Deng, X.W., Wu, W. and Xue, Y. 2008. F-box protein DOR functions as a novel inhibitory factor for abscisic acid-induced stomatal closure under drought stress in Arabidopsis. Plant Physiology. 148(4): 2121-2133. https://doi.org/10.1104/pp.108.126912
  27. Zheng, L., Lan, P., Shen, R.F. and Li, W.F. 2014. Proteomics of aluminum tolerance in plants. Proteomics. 14:566-578. https://doi.org/10.1002/pmic.201300252

Cited by

  1. Legume proteomics: Progress, prospects, and challenges vol.16, pp.2, 2016, https://doi.org/10.1002/pmic.201500257
  2. Aluminum Toxicity-Induced Alterations of Leaf Proteome in Two Citrus Species Differing in Aluminum Tolerance vol.17, pp.7, 2016, https://doi.org/10.3390/ijms17071180