Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
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Effects of Clematidis Radix Pharmacopuncture Injected at Sinsu ($BL_{23}$) on Neuropathic Pain in Rats

신유(腎兪)($BL_{23}$) 위령선약침이 백서(白鼠)의 L5 Spinal Nerve의 결찰로 유발된 신경병리성 동통에 미치는 영향

  • Kim, Sung Phil (Acupuncture, Moxibustion & Meridian Research Group, Department of Medical Research, Korea Institute of Oriental Medicine) ;
  • Choi, Sun Mi (Acupuncture, Moxibustion & Meridian Research Group, Department of Medical Research, Korea Institute of Oriental Medicine) ;
  • Kim, Jeong Eun (Acupuncture, Moxibustion & Meridian Research Group, Department of Medical Research, Korea Institute of Oriental Medicine) ;
  • Kim, Joo Hee (Acupuncture, Moxibustion & Meridian Research Group, Department of Medical Research, Korea Institute of Oriental Medicine) ;
  • Shin, Kyung Min (Acupuncture, Moxibustion & Meridian Research Group, Department of Medical Research, Korea Institute of Oriental Medicine) ;
  • Kim, Jae Hong (Department of Acupuncture and Moxibustion Medicine, College of Oriental Medicine, Dongshin University)
  • 김성필 (한국한의학연구원 의료연구본부 침구경락연구센터) ;
  • 최선미 (한국한의학연구원 의료연구본부 침구경락연구센터) ;
  • 김정은 (한국한의학연구원 의료연구본부 침구경락연구센터) ;
  • 김주희 (한국한의학연구원 의료연구본부 침구경락연구센터) ;
  • 신경민 (한국한의학연구원 의료연구본부 침구경락연구센터) ;
  • 김재홍 (동신대학교 한의과대학 침구의학교실)
  • Received : 2014.11.10
  • Accepted : 2014.11.23
  • Published : 2014.12.20
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Abstract

Objectives : The purpose of this study is to examine if Clematidis Radix(CR) pharmacopuncture may be effective to the neuropathic pain(mechanical allodynia) in a rat model of neuropathic pain. Methods : To produce the model of neuropathic, L5 spinal nerve was ligated by 6-0 silk thread. After neuropathic surgery, the author examined if the animals exhibited the plantar withdrawal response of allodynia. The plantar withdrawal response was assessed by dynamic plantar aesthesiometer three days after the neuropathic surgery, CR pharmacopuncture was injected at $BL_{23}$ 1time/week for 6 weeks. After that, the author examined the plantar withdrawal response of rats' leg by dynamic plantar aesthesiometer. And also the author examined mGluR5, Bax, Bcl-2, Bax/Bcl-2 ratio in spinal cord, and c-Fos. Also the author observed the change of aspartate aminotransferase(AST), alanine aminotransferase(ALT) count in the blood serum of neuropathic rats. Results : 1. The withdrawal response of allodynia decreased in the PT3, PT4 group as compared with control group. 2. The mGluR5 increased in the PT1, PT2, PT3, and PT4 group. 3. The Bax and Bax/Bcl-2 ratio decreased in the PT4 group. 4. The c-Fos increased in the PT1 group, and decreased in the PT4 group. 5. The changes of AST in blood serum decreased in the every group excluded control group and the changes of ALT in blood serum isn't shown the signigicant change. Conclusions : These results are suggested that CR pharmacopuncture at BL23 decreased mechanical allodynia, can act on anti-apoptotic, neuroprotective effects and liver fuction in the model of neuropathic pain.

Keywords

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