Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
권호 표지
DOI QR코드

DOI QR Code

Inhibitory Effect of Fractionated Trapa Japonica Extracts on UVB-induced Skin Photoaging

능실 추출물 및 그 분획물의 피부 광노화 억제 효능

  • Received : 2014.06.10
  • Accepted : 2014.08.05
  • Published : 2014.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Ultraviolet B (UVB) is a primary environmental factor that induces adverse effects on skin such as photoaging, skin burn and cancer. UVB also increases the expression of $11{\beta}$-hydroxysteroid dehydrogenase type 1 ($11{\beta}-HSD1$), which converts inactive cortisone to active cortisol in response to a variety of stressors in target tissues. Thus, we have screened new herbal extracts that suppress $11{\beta}-HSD1$ expression induced by UVB in human dermal fibroblasts. We also investigated whether Trapa japonica (TJ) extract and its fractions inhibit UVB-induced photoaging in Hs68 cells and 3D skin model. Results showed that TJ extract inhibited the increase of $11{\beta}-HSD1$ expression in UVB-exposed Hs68 cells significantly. TJ extract and its fractions not only inhibited $11{\beta}-HSD1$ expression, but also suppressed the increase of matrix metalloproteinases (MMP-1, 3, 9) and proinflammatory cytokines (IL-6, 8) in UVB-irritated Hs68 cells. TJ extract also inhibited MMP-1 expression in UVB-irritated 3D skin model. In addition, TJ extract recovered UVB-induced decreases of epidermal thickness and PCNA-positive cells in 3D skin model. Taken together, these results suggest that TJ extract and its fractions inhibit UVB-induced skin photoaging by interfering with $11{\beta}-HSD1$ and MMPs.

자외선은 피부의 구조적, 생리학적 변화를 일으키는 대표적인 외부 환경 인자로서 광노화, 일광화상 및 피부암을 일으키는 원인이 된다. 이러한 자외선은 외부적인 스트레스 자극 인자로 작용하여 피부 세포 내에서 비활성 코르티손을 활성 코르티솔로 전환시키는 효소인 $11{\beta}$-hydroxysteroid dehydrogenase type 1 ($11{\beta}-HSD1$)의 발현 및 활성을 증가시킨다. 이에 본 연구에서는 UVB에 의해 증가된 $11{\beta}-HSD1$의 발현을 효과적으로 억제할 수 있는 천연 추출물을 발굴하고자 하였다. 사람 섬유아세포에 다양한 천연물을 스크리닝한 결과, 능실 추출물이 유의한 효과가 있음을 확인하고, 능실 추출물을 추가 분획하여 사람 섬유아세포와 3D skin model에서 피부 광노화 억제 소재로서의 가능성을 확인하였다. 능실 추출물 및 분획물은 섬유아세포에서 $11{\beta}-HSD1$의 발현을 억제함과 동시에 자외선에 의한 matrix metalloproteinase (MMP)-1, 3, 9 및 염증성 싸이토카인(IL-6, 8)의 발현 증가를 억제하였다. 또한, 3D skin model을 이용한 평가에서, 능실 추출물은 UVB에 의한 MMP-1 단백질 발현을 억제하였고, UVB에 의한 표피 두께 감소 및 각질형성세포의 증식 감소를 회복시켰다. 따라서, 본 연구 결과로부터 능실 추출물 및 분획물은 UVB에 의한 $11{\beta}-HSD1$의 발현 증가와 이에 수반하는 광노화를 효과적으로 예방함을 확인하였다.

Keywords

References

  1. G. Rhie, M. H. Shin, J. Y. Seo, W. W. Choi, K. H. Cho, K. H. Kim, K. C. Park, H. C. Eun, and J. H. Chung, Aging- and photoaging-dependent changes of enzymic and noenzyme antioxidants in the epidermis and dermis of human skin in vivo, J. Invest. Dermatol., 117(5), 1212 (2001). https://doi.org/10.1046/j.0022-202x.2001.01469.x
  2. E. C. Naylor, R. E. B. Watson, and M. J. Sherratt, Molecular aspects of skin aging, Maturitas, 69(3), 249 (2011). https://doi.org/10.1016/j.maturitas.2011.04.011
  3. J. Y. Seo, E. K. Kim, S. H. Lee, K. C. Park, K. H. Kim, H. C. Eun, and J. H. Chung, Enhanced expression of cyclooxygenase-2 by UV in aged human skin in vivo, Mech. Ageing Dev., 124(8-9), 903 (2003). https://doi.org/10.1016/S0047-6374(03)00150-7
  4. J. M. Waller and H. I. Maibach, Age and skin structure and function, a quantitative approach (II): protein, glycosaminoglycan, water, and lipid content and structure, Skin Res. Technol., 12(3), 145 (2006). https://doi.org/10.1111/j.0909-752X.2006.00146.x
  5. M. Yaar and B. A. Gilchrest, Photoaging: mechanism, prevention and therapy, Br. J. Dermatol., 157(5), 874 (2007). https://doi.org/10.1111/j.1365-2133.2007.08108.x
  6. M. Ichihashi, M. Ueda, A. Budiyanto, T. Bito, M. Oka, M. Fukunaga, K. Tsuru, and T. Horikawa, UV-induced skin damage, Toxicology, 189(1-2), 21 (2003). https://doi.org/10.1016/S0300-483X(03)00150-1
  7. F. Afraq, V. M. Adhami, and H. Mukhtar, Photochemoprevention of ultraviolet B signaling and photocarcinogenesis, Mutat. Res., 571(1-2), 153 (2005). https://doi.org/10.1016/j.mrfmmm.2004.07.019
  8. P. Brenneisen, J. Wenk, L. O. Klotz, M. Wlascheck, K. Briviba, T. Krieg, H. Sies, and K. Scharffetter-Kochanek, Central role of ferrous/ferric iron in the ultraviolet B irradiation-mediated signaling pathway leading to increased interstitial collagenase (matrix-degrading metalloprotease (MMP)-1) and stromelysin-1 (MMP-3) mRNA level in cultured human dermal fibroblasts, J. Biol. Chem., 273(9), 5279 (1998). https://doi.org/10.1074/jbc.273.9.5279
  9. A. Tiganescu, A. A. Tahrani, S. A. Morgan, and M. Otranto, A. Desmouliere, L. Abrahams, Z. Hassan-Smith, E. A. Walker, E. H. Rabbit, M. S. Cooper, K. Amrein, G. G. Lavery, and P. M. Stewart, $11{\beta}$-Hydroxysteroid dehydrogenase blockade prevents age-induced skin structure and function defects, J. Clin. Invest., 123(7), 3051 (2013). https://doi.org/10.1172/JCI64162
  10. A. Tiganescu, E. A. Walker, R. S. Hardy, A. E. Mayes, and P. M. Stewart, Localization, age- and site-dependent expression, 11beta-hydroxysteroid dehydrogenase type 1 in skin, J. Invest. Dermatol., 131(1), 30 (2011). https://doi.org/10.1038/jid.2010.257
  11. M. Wamil and J. R. Seckl, Inhibition of 11beta-hydroxysteroid dehydrogenase type 1 as a promising therapeutic target, Drug Discov. Today, 12(13-14), 504 (2007). https://doi.org/10.1016/j.drudis.2007.06.001
  12. J. F. Michelet, C. Olive, E. Rieux, D. Fagot, L. Simonetti, J. B. Galey, M. Dalko-Csiba, B. A. Bernard, and R. Pereira, The anti-ageing potential of a new jasmonic acid derivative (LR2412): in vitro evaluation using reconstructed epidermis episkinTM, Exp. Dermatol., 21(5), 398 (2012). https://doi.org/10.1111/j.1600-0625.2012.01480.x
  13. M. Demerjian, E. H. Choi, M. Q. Man, S. Chang, P. M. Elias, and K. R. Feingold, Activators of PPARs and LXR decrease the daverse effects of exogenous glucocorticoids on the epidermis, Exp. Dermatol., 18(7), 643 (2009). https://doi.org/10.1111/j.1600-0625.2009.00841.x
  14. A. Paldo and M. Selman, MMP-1: the elder of the family, Int. J. Biochem. Cell Biol., 37(2), 283 (2005). https://doi.org/10.1016/j.biocel.2004.06.017
  15. J. S. Scott, F. W. Goldberg, and A. V. Turnbull, Medicinal chemistry of inhibitors of $11{\beta}$ -hydroxysteroid dehydrogenase type 1 ($11{\beta}$-HSD1), J. Med. Chem., 57(11), 4466 (2014). https://doi.org/10.1021/jm4014746
  16. M. Terao, H. Murota, A. Kimura, A. Kato, A. Ishikawa, K. Igawa, E. Miyoshi, and I. Katayama, $11{\beta}$-hydroxysteroid dehydrogenase-1 is a novel regulator of skin homeostasis and a candidate target for promoting tissue repair, PLoS ONE, 6(9), e25039 (2011). https://doi.org/10.1371/journal.pone.0025039
  17. H. Y. Lim, N. Muller, M. J. Herold, J. Brandt, and H. M. Reichardt, Glucocorticoids exert opposing effects on macrophage function dependent on their concentration, Immunology, 122(1), 47 (2007). https://doi.org/10.1111/j.1365-2567.2007.02611.x
  18. M. R. K. Dasu, R. E. Barrow, M. Spies, and D. N. Herndon, Matrix metalloproteinase expression in cytokine stimulated human dermal fibroblasts, Burns, 29(6), 527 (2003). https://doi.org/10.1016/S0305-4179(03)00154-2
  19. L. R. Luckette and R. M. Gallucci, Interleukin-6 (IL-6) modulates migration and matrix metalloproteinase function in dermal fibroblasts from IL-6KO mice, Br. J. Dermatol., 156(6), 1163 (2007). https://doi.org/10.1111/j.1365-2133.2007.07867.x
  20. M. A. Farage, K. W. Miller, P. Elsner, and H. I. Maibach, Characteristics of the aging skin, Adv. Wound Care, 2(1), 5 (2013). https://doi.org/10.1089/wound.2011.0356
  21. C. Skobowiat, R. M. Sayre, J. C. Dowdy, and A. T. Slominski, Ultraviolet radiation regulates cortisol activity in a waveband-dependent manner in human skin ex vivo, Br. J. Dermatol., 168(3), 595 (2012).

Cited by

  1. Chemical composition, health effects, and uses of water caltrop vol.49, 2016, https://doi.org/10.1016/j.tifs.2016.01.009
  2. Antioxidant and Antiproliferative Activity of Extracts from Water Chestnut (Trapa japonica Flerow) vol.24, pp.1, 2016, https://doi.org/10.7783/KJMCS.2016.24.1.14