Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Isolation and Identification of Antifungal Compounds from Eugenia caryophyllata Extracts

정향 추출물로부터 항진균성 물질의 분리 및 동정

  • Lee, Jin-Man (Department of Food Science and Technology and Basic Science Institute, Hoseo University) ;
  • Hur, Sang-Sun (Department of Food Science and Biotechnology, Joongbu University)
  • 이진만 (호서대학교 식품생물공학과) ;
  • 허상선 (중부대학교 식품생명과학과)
  • Received : 2014.11.25
  • Accepted : 2014.12.26
  • Published : 2014.12.30
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Abstract

Antifungal properties of clove(Eugenia caryophyllata) against food spoilage microorganism, Penicillium rugullosum IFO 4683 was investigated. Antifungal activity of the essential oil was as equivalent as potassium metabisulfite and myconazole. The clove extracts was fractionated to hexane, chloroform, ethyl acetate, butanol and water fraction. Hexane fraction showed the highest inhibitory effect on the Penicillium rugullosum IFO 4683. Hexane fraction was further fractionated by silica gel column chromatography and thin layer chromatography(TLC). The antifungal compound was isolated from their fractions and their chemical structures were identified as eugenol, eugenol acetate and chavicol by EI-MS, $^1H$-NMR and $^{13}C$-NMR.

식품부패미생물에 대한 정향추출물의 항진균력을 확인하고 유효성분을 분리한 후 그 구조를 확인하였다. 정향 추출물은 대표적인 항진균제인 메타중아황산칼륨 및 myconazole과 비슷한 항진균 활성을 나타내었다. 용매분획을 실시한 결과 hexane fraction이 가장 항진균활성이 높은 것으로 나타났다. 이 hexane 분획을 silica gel column chromatography를 통해 분리하여 항진균활성이 가장 큰 분획물인 JH-1, JH-2-1 및 JH-2-2을 분리하였다. 이들 화합물은 EI-MS 와 $^1H$-NMR, $^{13}C$-NMR 결과로부터 활성물질의 구조를 동정하였으며 그 결과 JH-1은 eugenol, JH-2-1은 eugenol acetate 그리고 JH-2-2은 chavicol인 것으로 확인하였다.

Keywords

References

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