Korean Journal of Ecology and Environment (생태와환경)

The Korean Society of Limnology (한국수생태학회)
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Identification and Analysis of Geosmin Production Potential of Anabaena stain Isolated from North Han River using Genetic Methods

유전자 기법을 이용한 북한강 수역 Anabaena strain의 동정 및 Geosmin 생산 잠재성 분석

  • Kim, Keon Hee (Department of Environmental Science, Konkuk University) ;
  • Lim, Byung-Jin (Han River Environment Research Center, National Institute of Environmental Research) ;
  • You, Kyoung-A (Han River Environment Research Center, National Institute of Environmental Research) ;
  • Park, Myung-Hwan (Department of Environmental Science, Konkuk University) ;
  • Park, Jung Hwan (Department of Environmental Science, Konkuk University) ;
  • Kim, Baik-Ho (Department of Life Science, Hanyang University) ;
  • Hwang, Soon-Jin (Department of Environmental Science, Konkuk University)
  • 김건희 (건국대학교 환경과학과) ;
  • 임병진 (국립환경과학원 한강물환경연구소) ;
  • 유경아 (국립환경과학원 한강물환경연구소) ;
  • 박명환 (건국대학교 환경과학과) ;
  • 박정환 (건국대학교 환경과학과) ;
  • 김백호 (한양대학교 생명과학과) ;
  • 황순진 (건국대학교 환경과학과)
  • Received : 2014.11.20
  • Accepted : 2014.12.22
  • Published : 2014.12.31
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Abstract

This study was conducted to identify the bloom-forming Anabaena strains with two phenotypes (straight-type and coil-type) isolated from North Han River (Sambong-ri Joam-myun) using 16S rDNA sequence. The odor material producing potential was also examined using the geosmin-synthesizing gene cluster. Despite of striking morphological difference of the two stains, resembling A. circinalis and A. crassa species, the phylogenetic analysis using 16S rDNA identified the both strains as a single species of A. circinalis with high genetic similarity (98%~100%). Also, two Anabaena strains showed to possess the geosmin-synthesizing gene cluster, indicating that they are capable of producing the odor substance. This study is the first report that provides the direct evidence of geosmin production in the gene level by A. circinalis in Korea, and provides important basic information to identify the source alga of geosmin increase and its management in North Han River.

본 연구는 최근 북한강 수계에서 번성하고 있는 Anabaena strain의 16S rDNA 염기서열을 이용한 종 수준의 동정과 geosmin 합성 유전자의 탐침을 통해 이취미 물질의 잠재 생산능력을 분석하였다. 현장(경기도 양평군 조암면 삼봉리 수역)에서 분리 배양한 Anabaena는 직선형과 나선형 두 가지의 형태적 변이를 보였다. 이들은 세포의 크기와 사상체에서 형태적 차이를 나타냈으며, A. circinalis 및 A. crassa와 유사한 형태적 특징들을 보여주었다. 그러나 16S rDNA 계통수 및 유연관계를 분석한 결과, 직선형과 나선형 모두 동일한 A. circinalis 종으로 확인되었다(98%~100%의 유전적 유사도). 또한 직선형과 나선형 strain 모두에서 geosmin을 합성하는 유전자 구간이 발견되어, 북한강 수계에 존재하는 Anabaena circinalis는 종의 형태적 변이에 관계없이 geosmin을 생산할 수 있음을 보여주었다. 본 연구의 결과는 유전자 수준에서 A. circinalis의 geosmin 생산에 대한 직접적인 증거를 제공하는 국내 최초의 보고로서 북한강 수계에서 geosmin 증가의 원인종 확인 및 관리에 중요한 자료를 제공한다.

Keywords

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