Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
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Effect of Chlorella vulgaris CHK0008 Fertilization on Enhancement of Storage and Freshness in Organic Strawberry and Leaf Vegetables

Chlorella vulgaris CHK0008 시비가 유기농 딸기와 엽채소의 저장성과 신선도 향상에 미치는 영향

  • Kim, Min-Jeong (Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Shim, Chang-Ki (Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Yong-Ki (Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Park, Jong-Ho (Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Hong, Sung-Jun (Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Ji, Hyeong-Jin (Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Han, Eun-Jung (Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Yoon, Jung-Chul (Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration)
  • 김민정 (농촌진흥청 국립농업과학원 유기농업과) ;
  • 심창기 (농촌진흥청 국립농업과학원 유기농업과) ;
  • 김용기 (농촌진흥청 국립농업과학원 유기농업과) ;
  • 박종호 (농촌진흥청 국립농업과학원 유기농업과) ;
  • 홍성준 (농촌진흥청 국립농업과학원 유기농업과) ;
  • 지형진 (농촌진흥청 국립농업과학원 유기농업과) ;
  • 한은정 (농촌진흥청 국립농업과학원 유기농업과) ;
  • 윤종철 (농촌진흥청 국립농업과학원 유기농업과)
  • Received : 2014.06.16
  • Accepted : 2014.07.14
  • Published : 2014.12.31
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Abstract

This study aimed to enhance storage and freshness of strawberry fruits and foliage vegetables by spray treatment with Chlorella vulgaris as a bio-fertilizer. The tested strain, C. vulgaris CHK0008, was isolated from an organically cultivated rice paddy and identified as C. vulgaris by its morphology and 18S rDNA and 23S rDNA sequence homology. We successfully cultured C. vulgaris CHK0008 in BG11 modified medium (BG11MM) and adjusted $2.15{\times}10^6cell/mL$ C. vulgaris CHK0008 to one OD value by measuring the optical density at 680 nm using a UV-vis spectrophotometer. The soluble solid content of 'Seolhyang' and 'Yukbo' strawberry fruits treated by spray application with C. vulgaris CHK0008 was enhanced by 22.2% and 11.5% respectively, compared to untreated controls. Additionally, the decay rates of treated 'Seolhyang' and 'Yukbo' strawberry fruits decreased 63.8% and 74.4% respectively, compared to untreated control. Surface color changes and chlorosis of leaves in leaf vegetables such as lettuce, kale, red ornamental kale, white ornamental kale and beet were observed in samples treated with water spray for 10 days after cold storage. However, the decay rate of leafy vegetables treated with foliar application of 25% C. vulgaris CHK0008 liquid culture was significantly decreased compared to that of the untreated control during storage at $4^{\circ}C$.

이 연구는 생물비료로서 클로렐라(Chlorella vulgaris) 분무처리에 의한 유기농 딸기와 엽채류의 신선도와 저장성 향상을 목표로 하였다. 클로렐라 균주 CHK0008은 유기농 벼재배 논의 담수에서 분리하였으며 형태적 특징과 18S rDNA와 23S rDNA 염기서열 비교에 의해 C. vulgaris로 동정하였다. 실험에 사용한 C. vulgaris CHK0008 균주는 BG11 변형배지(BGMM)에서 잘 배양되었으며 UV/VIS 분광광도계로 680nm에서 흡광도를 측정하였을 때 1 OD의 C. vulgaris CHK0008는 $2.15{\times}10^6cell/mL$ 농도로 개산하였다. 클로렐라(C. vulgaris)의 엽면처리구와 무처리구를 비교하였을 때 '설향'과 '육보' 딸기 품종의 당도가 각각 22.2%, 11.5% 향상되었다. 또한 엽면처리구의 '설향'과 '육보' 딸기 품종의 부패율은 무처리구에 비해 각각 63.8%와 74.4% 감소하였다. 엽채류인 상추, 케일, 붉은 케일, 흰 케일, 비트의 엽색 변화 및 백화현상이 저온저장 10일 후 물만 분무 처리한 무처리에서 관찰되었다. 그러나 25% C. vulgaris 배양액을 엽면 처리한 엽채류를 $4^{\circ}C$에서 저장하는 동안 부패율이 무처리에 비해 현저히 감소하였다.

Keywords

References

  1. Andersen, R.A. 2005. Algal culturing techniques. Elsevier Academic Press, San Diego, C.A., USA. p. 239-287.
  2. Bai, S.C. and Y.T. Cha. 1997. Comparison of growth and body composition in olive flounder larvae (Paralichthys olivaceus) fed domestic experimental and imported commercial microparticulated diets. J. Aquaculture 10:87-95.
  3. Becker, E.W. 1994. Microalgae-biotechnology and microbiology, Cambridge studies in biotechnology Vol. 10. Cambridge University Press, Cambridge, UK.
  4. Boloor, K.K., J.P. Kamat, and T.P.A. Devasagayam. 2000. Chlorophyllin as a protector of mitochondrial membranes against ${\gamma}$-radiation and photosensitization. Toxicology 155:63-71. https://doi.org/10.1016/S0300-483X(00)00278-X
  5. Cha, J.Y., J.W. Kim, B.K. Park, H.J. Jin, S.Y. Kim, and Y.S. Cho. 2008. Isolation and identification of Chlorella sp. CMS-1 and the chemical composition of its hot water extract. J. Life Sci. 18:1723-1727. https://doi.org/10.5352/JLS.2008.18.12.1723
  6. Cho, K.J., S.H. Han, B.Y. Kim, S.G. Hwang, K.K. Park, K.H. Yang, and A.S. Chung. 2000. Chlorophyllin suppression of Lipopolysaccharide-induced nitric oxide production in RAW 264.7 cells. Toxicol. Appl. Pharm. 166:120-127. https://doi.org/10.1006/taap.2000.8958
  7. Chung, S.K. and S.H. Cho. 2003. Preservative effect of natural antimicrobial substances used as steeping and packing agent on postharvested strawberries. Korean J. Food Preserv. 10:37-40.
  8. Deng, X.Y., K. Gao, and J.L. Sun. 2012. Growth characteristics and nutrient removal properties of the freshwater cyanobacterium Synechococcus sp. PCC7942 under different kinds of nitrogen sources. African J. Mic. Res. 6:4927-4931.
  9. Heo, J.Y., H.J. Shin, D.H. Oh, S.K. Cho, C.J. Yang, I.K. Kong, S.S. Lee, K.S. Choi, S.H. Choi, S.C. Kim, H.Y. Choi, and I.H. Bae. 2006. Quality properties of appenzeller cheese added with Chlorella. Korean J. Food Sci. Ani. Resour. 26:525-531.
  10. Hsu, C.Y., C.M. Yang, C.M. Chen, P.Y. Chao, and S.P. Hu. 2005. Effects of chlorophyll-related compounds on hydrogen peoxide induced DNA damage within human lymphocytes. J. Agric. Food Chem. 53:2746-2750. https://doi.org/10.1021/jf048520r
  11. Hwang, Y.S. and J.H. Ku. 2004. Effect of high molecular weight chitosan on the quality and decay of strawberry fruits. J. Agri. Sci. Chungnam Natl. Univ. Korea 31:77-86.
  12. In, B.C. and J.G. Kim. 2008. Effect of precooling and harvesting at different times on respiration, browning and microbial growth of fresh-cut iceberg lettuce. Kor. J. Hort. Sci. Technol. 26:258-264.
  13. Jeong, J.W., J.H. Jo, D.J. Kwon, and Y.B. Kim. 1990. Studies on the low temperature storage of strawberry pulp and red pepper paste by cryoprotectants. Kor. J. Food Sci. Technol. 22:434-438.
  14. Kang, H.K., H.C. Choi, D.W. Kim, J. Hwangbo, J.C. Na, H.T. Bang, D.W. Kim, M.J. Kim, M.M.H. Mushtaq, R. Parvin, and J.H. Kim. 2013. Effect of dietary chlorella supplementation on growth performance, immune response, and intestinal micro flora concentration of broiler chickens. Korean J. Poult. Sci. 40:271-276. https://doi.org/10.5536/KJPS.2013.40.3.271
  15. Kim, S.U. and H.J. Lee. 2013. A study of consumer characteristics in organic agriculture market. J. Kor. Industrial Economics 6:1247-1270.
  16. Kim, B.S., D.C. Kim, S.E. Lee, G.B. Nahm, and J.W. Jeong. 1995. Freshness prolongation of crisphead lettuce by vacuum cooling and cold-chain system. Kor. J. Food Sci. Technol. 27:546-554.
  17. Kim, C.G., H.G. Jeong, and D.H. Moon. 2012. Current markets and production status of environmental-friendly agricultural product in domestic and foreign in 2012. Sustainable Agriculture Research 14:21-45.
  18. Kim, C.G., H.G. Jeong, and D.H. Moon. 2013. Current markets and production status of environmental-friendly agricultural product in domestic and foreign. Korea Rural Economic Institute Agri-Policy Focus 55:1-26.
  19. Kim, D.K., S.J. Kim and K.H. Lee. 2011. The effect of food choice motive on attitude and intention of purchasing organic food. Korean J. Food Culture 26:506-512.
  20. Kim, S.S., M.K. Park, N.S. Oh, D.C. Kim, M.S. Han, and M.J. In. 2003. Studies on quality characteristics and shelf-life of chlorella soybean (Tofu). J. Korean Soc. Agric. Chem. Biotechnol. 46:12-15.
  21. Korea Rural Economic Institute (KREI). 2010. Agricultural outlook 2010 (I). KREI, Seoul, Korea.
  22. Lee, H.E. and H.C. Kim. 2012. The structural relationship of perceived value, perceived quality, environmental commitment, purchasing attitude and purchasing intention of organic food consumer. J. Tourism Sci. 99:295-318.
  23. Lee, H.H., S.I. Hong, and D.N. Kim. 2013. Storage quality of Sulhyang strawberries as affected by high $O_2$ atmosphere packaging. Korean J. Food Sci. Technol. 45:191-198. https://doi.org/10.9721/KJFST.2013.45.2.191
  24. Lee, Y.S., S. Kim, and D.H. Kim. 2009. Current status of fresh-cut market in Korea and stimulus measures. Korea Rural Economic Ins. Rpt. 602:16-19.
  25. Park, J.E. and S.J. Hwang. 2010. Effect of precooling and storage temperatures on the post-harvest management of the fruits in 'Maehyang' and 'Soogyeong' strawberries for export. J. Bio-Environ. Cont. 19:366-371.
  26. Park, M.K., J.M. Lee, C.H. Park, and M.J. In. 2002. Quality characteristics of Sulgidduk containing Chlorella powder. J. Korean Soc. Food Sci. Nutr. 31:225-229. https://doi.org/10.3746/jkfn.2002.31.2.225
  27. Raposo, R.M., M. Filomena, C.D. Morais and R.M. Santos. 2011. Chlorella vulgaris as soil amendment: Influence of encapsulation and enrichment with rhizobacteria. Int. J. Agr. Bio. 13:719-724.
  28. Shin, J.W. and Y.S. Hwang. 2001. Study on cultivation and distribution of strawberry. Proceedings of Agriculture and Life Science, Chungnam University, Daejeon, Korea. p. 257-262.
  29. Stanier, R.Y., R. Kunisawa, M. Mandel, and G. Cohen-Bazire. 1971. Purification and properties of unicellular blue-green algae (order Chroococcales). Bact. Res. 35:171-205.
  30. Stirk, W.A., P. Balint, D. Tarkowska, O. Novak, G. Maroti, K. Ljung, V. Tureckova, M. Strnad, V. Ordog, and J. van Staden. 2014. Effect of light on growth and endogenous hormones in Chlorella minutissima (Trebouxiophyceae). Plant Physiol. Biochem. 79:66-76. https://doi.org/10.1016/j.plaphy.2014.03.005
  31. Tomaselli, L. 2004. The Microalgal cell, p. 11-17. In: A. Richmond (ed). Handbook of microalgal culture. Blackwell Publishing Company, Ames, Iowa, USA.
  32. Travieso, L., A. Pellon, F. Benitez, E. Sanchez, R. Borja, N. O'Farrill, and P. Weiland. 2002. Bioalga reactor: preliminary studies for heavy metals removal. Biochem. Engin. J. 12:87-91. https://doi.org/10.1016/S1369-703X(02)00045-1
  33. Zheng, Y., Z. Yang, and X. Chen. 2008. Effect of high oxygen atmospheres on fruit decay and quality in Chinese bayberries, strawberries and blueberries. Food Cont. 19:470-474. https://doi.org/10.1016/j.foodcont.2007.05.011

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