Development and Reproduction (한국발생생물학회지:발생과생식)

The Korean Society of Developmental Biology (한국발생생물학회)
권호 표지
DOI QR코드

DOI QR Code

Expression of Tight Junction Molecule In The Human Serum-Induced Aggregation of Human Abdominal Adipose-Derived Stem Cells In Vitro

  • Yoon, A Young (Dept. of Biotechnology, Seoul Women's University) ;
  • Yun, Sujin (Dept. of Biotechnology, Seoul Women's University) ;
  • Yang, HyeJin (Dept. of Biotechnology, Seoul Women's University) ;
  • Lim, Yoon Hwa (Dept. of Biotechnology, Seoul Women's University) ;
  • Kim, Haekwon (Dept. of Biotechnology, Seoul Women's University)
  • Received : 2014.11.07
  • Accepted : 2014.11.17
  • Published : 2014.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Previously we have shown that human abdominal adipose derived-stem cells (ADSCs) could aggregate during the high-density culture in the presence of human serum (HS). In the present study, we observed that human cord blood serum (CBS) and follicular fluid (HFF) also induced aggregation. Similarly, porcine serum could induce aggregation whereas bovine and sheep sera induced little aggregation. qRT-PCR analyses demonstrated that, compared to FBS-cultured ADSCs, HS-cultured cells exhibited higher level of mRNA expression of CLDN3, -6, -7, -15, and -16 genes among the tight junction proteins. ADSCs examined at the time of aggregation by culture with HS, BSA, HFF, CBS, or porcine serum showed significantly higher level of mRNA expression of JAM2 among JAM family members. In contrast, cells cultured in FBS, bovine serum or sheep serum, showed lower level of JAM2 expression. Immunocytochemical analyses demonstrated that the aggregates of HS-cultured cells (HS-Agg) showed intense staining against the anti-JAM2 antibody whereas neither non-aggregated cells (HS-Ex) nor FBS-cultured cells exhibited weak staining. Western blot results showed that HS-Agg expressed JAM2 protein more prominently than HS-Ex and FBS-cultured cells, both of latter reveled weaker intensity. These results suggest that the aggregation property of ADSCs during high-density culture would be dependent on the specific components of serum, and that JAM2 molecule could play a role in the animal sera-induced aggregation in vitro.

Keywords

References

  1. Aldahmash A, Haack-Sorensen M, Al-Nbaheen M, Harkness L, Abdallah BM, Kassem M (2011) Human serum is as efficient as fetal bovine serum in supporting proliferation and differentiation of human multipotent stromal (mesenchymal) stem cells in vitro and in vivo. Stem Cell Rev 7:860-868. https://doi.org/10.1007/s12015-011-9274-2
  2. Bazzoni G. (2003) The JAM family of junctional adhesion molecules. Curr Opin Cell Biol 15:525-530. https://doi.org/10.1016/S0955-0674(03)00104-2
  3. Ben-David U, Nudel N, Benvenisty N. (2013) Immunologic and chemical targeting of the tight junction protein Claudin-6 eliminates tumorigenic human pluripotent stem cells. Nat Commun 4:1992.
  4. Bhumiratana S, Eton RE, Oungoulian SR, Wan LQ, Ateshian GA, Vunjak-Novakovic G (2014) Large, stratified, and mechanically functional human cartilage grown in vitro by mesenchymal condensation. Proc Natl Acad Sci U S A 111:6940-6945. https://doi.org/10.1073/pnas.1324050111
  5. Brizzi MF, Tarone G, Defilippi P (2012) Extracellular matrix, integrins, and growth factors as tailors of the stem cell niche. Curr Opin Cell Biol 24:645-651. https://doi.org/10.1016/j.ceb.2012.07.001
  6. Chen G, Shi X, Sun C, Li M, Zhou Q, Zhang C, Huang J, Qiu Y, Wen X, Zhang Y, Zhang Y, Yang S, Lu L, Zhang J, Yuan Q, Lu J, Xu G, Xue Y, Jin Z, Jiang C, Ying M, Liu X (2013) VEGF-mediated proliferation of human adipose tissue-derived stem cells. PLoS One 8: e73673. https://doi.org/10.1371/journal.pone.0073673
  7. Frith JE, Mills RJ, Hudson JE, Cooper-White JJ (2012) Tailored integrin-extracellular matrix interactions to direct human mesenchymal stem cell differentiation. Stem Cells Dev 21:2442-2456. https://doi.org/10.1089/scd.2011.0615
  8. Galderisi U1, Giordano A (2014) The gap between the physiological and therapeutic roles of mesenchymal stem cells. Med Res Rev 34:1100-1126. https://doi.org/10.1002/med.21322
  9. Garrido-Urbani S, Bradfield PF, Imhof BA (2014) Tight junction dynamics: the role of junctional adhesion molecules (JAMs). Cell Tissue Res 355:701-715. https://doi.org/10.1007/s00441-014-1820-1
  10. Ghislin S, Obino D, Middendorp S, Boggetto N, Alcaide-Loridan C, Deshayes F (2011) Junctional adhesion molecules are required for melanoma cell lines transendothelial migration in vitro. Pigment Cell Melanoma Res 24:504-511. https://doi.org/10.1111/j.1755-148X.2011.00856.x
  11. Hahn JY, Cho HJ, Kang HJ, Kim TS, Kim MH, Chung JH, Bae JW, Oh BH, Park YB, Kim HS (2008) Pretreatment of mesenchymal stem cells with a combination of growth factors enhances gap junction formation, cytoprotective effect on cardiomyocytes, and therapeutic efficacy for myocardial infarction. J Am Coll Cardiol 51:933-943. https://doi.org/10.1016/j.jacc.2007.11.040
  12. Le Blanc K, Frassoni F, Ball L, Locatelli F, Roelofs H, Lewis I, Lanino E, Sundberg B, Bernardo ME, Remberger M, Dini G, Egeler RM, Bacigalupo A, Fibbe W, Ringden O (2008) Developmental committee of the European group for blood and marrow transplantation mesenchymal stem cells for treatment of steroid resistant, severe, acute graft-versus-host disease: a phase II study. Lancet 371:1579-1586. https://doi.org/10.1016/S0140-6736(08)60690-X
  13. Li X, Yuan J, Li W, Liu S, Hua M, Lu X, Zhang H. (2013) Direct differentiation of homogeneous human adipose stem cells into functional hepatocytes by mimicking liver embryogenesis. J Cell Physiol 229:801-812.
  14. Ludwig RJ, Hardt K, Hatting M, Bistrian R, Diehl S, Radeke HH, Podda M, Schon MP, Kaufmann R, Henschler R, Pfeilschifter JM, Santoso S, Boehncke WH (2009) Junctional adhesion molecule (JAM)-B supports lymphocyte rolling and adhesion through interaction with alpha4beta1 integrin. Immunology 128:196-205. https://doi.org/10.1111/j.1365-2567.2009.03100.x
  15. Pereira J, Portron S, Dizier B, Vinatier C, Masson M, Sourice S, Galy-Fauroux I, Corre P, Weiss P, Fischer AM, Guicheux J, Helley D (2014) The in vitro and in vivo effects of a low-molecular weight fucoidan on the osteogenic capacity of human adipose-derived stromal cells. Tissue Eng Part A 20:275-284. https://doi.org/10.1089/ten.tea.2013.0028
  16. Song Y, Yun S, Yang HJ, Yoon AY, Kim H (2012) Aggregation of human eyelid adipose-derived stem cells by human body fluids. Dev Reprod 16:339-351. https://doi.org/10.12717/DR.2012.16.4.339
  17. Su RW, Jia B, Ni H, Lei W, Yue SL, Feng XH, Deng WB, Liu JL, Zhao ZA, Wang TS, Yang ZM (2012) Junctional adhesion molecule 2 mediates the interaction between hatched blastocyst and luminal epithelium: induction by progesterone and LIF. PLoS One 7:e34325. https://doi.org/10.1371/journal.pone.0034325
  18. Sun Z, Zhang Y, Yang S, Jia J, Ye S, Chen D, Mo F. (2012) Growth differentiation factor 5 modulation of chondrogenesis of self-assembled constructs involves gap junction-mediated intercellular communication. Dev Growth Differ 54:809-817. https://doi.org/10.1111/dgd.12009
  19. Valiunas V, Doronin S, Valiuniene L, Potapova I, Zuckerman J, Walcott B, Robinson RB, Rosen MR, Brink PR, Cohen IS (2004) Human mesenchymal stem cells make cardiac connexins and form functional gap junctions. J Physiol 555:617-626. https://doi.org/10.1113/jphysiol.2003.058719
  20. Wang L, Yang Y, Zhu Y, Ma X, Liu T, Zhang G, Fan H, Ma L, Jin Y, Yan X, Wei J, Li Y (2012) Characterization of placenta-derived mesenchymal stem cells cultured in autologous human cord blood serum. Mol Med Rep 6:760-766.
  21. Wang Y, Lui WY (2009) Opposite effects of interleukin-1alpha and transforming growth factor-beta2 induce stage-specific regulation of junctional adhesion molecule-B gene in Sertoli cells. Endocrinology 150:2404-2412. https://doi.org/10.1210/en.2008-1239
  22. Wong RC, Pera MF, Pebay A (2008) Role of gap junctions in embryonic and somatic stem cells. Stem Cell Rev 4:283-292. https://doi.org/10.1007/s12015-008-9038-9
  23. Yamada T, Kuramitsu K, Rikitsu E, Kurita S, Ikeda W, Takai Y (2013) Nectin and junctional adhesion molecule are critical cell adhesion molecules for the apico-basal alignment of adherens and tight junctions in epithelial cells. Genes Cells 18:985-998. https://doi.org/10.1111/gtc.12091
  24. Zhang B, Yang S, Sun Z, Zhang Y, Xia T, Xu W, Ye S (2011) Human mesenchymal stem cells induced by growth differentiation factor 5: an improved selfassembly tissue engineering method for cartilage repair. Tissue Eng Part C Methods 17:1189-1199. https://doi.org/10.1089/ten.tec.2011.0011