DOI QR코드

DOI QR Code

Development of the transgenic silkworm producing a improved green fluorescence cocoon

실용형질이 우수한 녹색 형광실크 형질전환 누에 개발

  • Piao, Yulan (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Kim, Sung-Wan (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Kim, Seong-Ryul (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Kim, Kee-Young (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Kang, Seok-Woo (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Goo, Tae-Won (Department of Biochemistry, School of Medicine, Dongguk University) ;
  • Choi, Kwang-Ho (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA)
  • 박옥란 (농촌진흥청 국립농업과학원 잠사양봉소재과) ;
  • 김성완 (농촌진흥청 국립농업과학원 잠사양봉소재과) ;
  • 김성렬 (농촌진흥청 국립농업과학원 잠사양봉소재과) ;
  • 김기영 (농촌진흥청 국립농업과학원 잠사양봉소재과) ;
  • 강석우 (농촌진흥청 국립농업과학원 잠사양봉소재과) ;
  • 구태원 (동국대학교 의과대학) ;
  • 최광호 (농촌진흥청 국립농업과학원 잠사양봉소재과)
  • Received : 2014.09.29
  • Accepted : 2014.10.19
  • Published : 2014.10.31

Abstract

On previous studies, we constructed a transgenic silkworm which produces the chimeric silk fused green fluorescent protein (EGFP), but the transgenic silkworm has decreased commercial feasible traits such as convenience of breeding and productivity of silk. In this study, we performed cross fertilization between green fluorescent silk transgenic silkworm and colored cocoon silkworm descents to make the transgenic the transgenic silkworm producing improved fluorescence cocoon. In the result, we found out a bit valuable cross fertilization manners ($female{\times}male$) in respect of silk productivity such as $T59B{\times}Jam26$, $Jam329{\times}T59W$, $T59W{\times}Jam329$, and $T59W{\times}Jam178$. The color-difference of offspring cocoons were measured according to different cross manners using by CIE Lab-based formulae with a X-rite VS450. In the result, the depth of green color of cocoons was a little high at cross manners as $Jam329{\times}T59W$, $T59W{\times}Jam178$. Meanwhile, the depth of yellow clolor of cocoons was remarkable at cross manners as $Jam178{\times}T59W$, $T59W{\times}Jam178$, respectively.

선행연구를 통하여, 형질전환 누에를 제작하는 원천기술을 개발하고 이를 통해 녹색 형광실크를 생산하는 형질전환 누에를 제작한 바 있다. 본 연구에서는 선행연구에서 개발된 녹색 형광실크 형질전환 누에와 국립농업과 학원이 보유하고 있는 누에 유전자원 중 유색견(絹) 누에품종을 이용하여 형질전환 누에와 유색견 누에품종 간 교차교배를 통한 잡종강세를 이용하여 고치 생산량 및 고치의 색채 등이 향상된 녹색 형광실크 형질전환 누에 1대 잡종(F1) 계통을 개발하였다. 본 연구를 통해 선발된 실용형질이 우수한 농가보급형 녹색 형광실크 형질전환 누에 1대 잡종(F1) 계통은 기존 형질전환 누에의 녹색 형광단백질 뿐 아니라 유색견 누에 특유의 천연 연록색도 함께 갖고 있어 별도의 염색이 필요 없는 차별화된 고급 패션의류나 벽지 등의 고품질 소재로 활용이 가능할 것으로 기대된다.

Keywords

References

  1. Imamura M, Nakai J, Inoue S, Guo XQ, Kanda T, Tamura T (2003) Target gene expression using the GAL4/UAS system in the silkworm Bombyx mori. Genetics 165, 13291340.
  2. Kim JR, Lee SJ, Kim SS (2005) Color differences of standard samples according to the their lightness levels. J of Korean Soc of Dyers and Finishers 17(2), 19-25.
  3. Kim NH (2004) A study on the color analysis of character by colour difference, Cartoon & Animation studies 8, 77-100.
  4. Kim SW, Kang MU, Kang SW, Yun EY, Choi KH, Kim SR, Park SW, Nho SK, Goo TW (2013a) Modification of the commercial silkworm eggs adeauate for silkworm transgenesis. J Seri Entomol Sci 51(1), 73-77.
  5. Kim SW, Yun EY, Choi KH, Kim SR, Park SW, Kang SW, Goo TW (2013b) Production of fluorescent green silk using fibroin H-chain expression system. J Seri Entomol Sci 51(2), 1-6.
  6. Kim SW, Yun EY, Choi KH, Kim SR, Park SW, Kang SW, Goo TW (2014) Expression of the blue fluorescent protein in fibroin H-chain of transgenic silkworm. J Seri Entomol Sci 52(1), 1-8.
  7. Kim SW, Yun EY, Choi KH, Kim SR, Park SW, Kang SW, Kwon OY, Goo TW (2012) Construction of fluorescent red silk using fibroin H-chain expression system. J Seri Entomol Sci 50(2), 87-92. https://doi.org/10.7852/jses.2012.50.2.87
  8. Kim YC (1982) Breeding, pp14-16, Hakmun Press, Seoul.
  9. Rika H, Tomita M, Yoshzato K (2006) The generation of germline transgenic silkworms for the production of biologically active recombinant fusion proteins of fibroin and human basic fibroblast growth factor. Biomaterials 27, 57155724.
  10. Satoshi Y, Zhu Z, Iaso K, Uchino K, Tamada Y, Tamura T,Asakura T (2007) Improving cell-adhesive properties ofrecombinant Bombyx mori silk by incorporation of collagenor fibronectin derived peptides produced by transgenicsilkworms. Biomacromolecules 8, 34873492.
  11. Tamura T, Thibert T, Royer C, Kanda T, Eappen A, Kamba M, Komoto N, Thomas JL, Mauchamp B, Chavancy G, Shirk P,Fraser M, Prudhomme JC, Couble P (2000) A piggyBacelement-deribed vector efficiently promoters germ-linetransformation in the silkworm, Bombyx mori L. NatBiotechnol 18, 8184.
  12. Tatematsu K, Kobayash I, Uchino K, Sezutsu H, Lizuka T, Yonemura N, Tamira T (2010) Construction of a binary transgenic gene expression system for recombinant protein production in the middle silk gland of the silkworm Bombyx mori. Transgenic Res 19, 473-487. https://doi.org/10.1007/s11248-009-9328-2

Cited by

  1. Single-dose oral toxicity study of genetically modified silkworm expressing EGFP protein in ICR mouse vol.43, pp.1, 2014, https://doi.org/10.7744/kjoas.20160013