The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Optimization of Extraction Conditions for Swertiamarin in Swertia japonica Makino

당약의 swertiamarin 분석을 위한 추출조건 최적화

  • Kim, Tae Hee (Herbal Medicine Resources Group, Korea Institute of Oriental Medicine) ;
  • Jang, Seol (Herbal Medicine Resources Group, Korea Institute of Oriental Medicine) ;
  • Lee, Ah Reum (Herbal Medicine Resources Group, Korea Institute of Oriental Medicine) ;
  • Lee, A Young (Herbal Medicine Resources Group, Korea Institute of Oriental Medicine) ;
  • Choi, Goya (Herbal Medicine Resources Group, Korea Institute of Oriental Medicine) ;
  • Kim, Ho Kyoung (Herbal Medicine Resources Group, Korea Institute of Oriental Medicine)
  • 김태희 (한국한의학연구원 한약자원그룹) ;
  • 장설 (한국한의학연구원 한약자원그룹) ;
  • 이아름 (한국한의학연구원 한약자원그룹) ;
  • 이아영 (한국한의학연구원 한약자원그룹) ;
  • 최고야 (한국한의학연구원 한약자원그룹) ;
  • 김호경 (한국한의학연구원 한약자원그룹)
  • Received : 2013.12.24
  • Accepted : 2014.01.21
  • Published : 2014.01.30
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Abstract

Objectives : Iridoid glycoside, swertiamarin is a well known bioactive component found in Swertia japonica Makino (SJ). In this study, we tried to optimize a suitable method which would extract swertiamarin effectively. Methods : Extraction of SJ was carried out by various conditions of time (5 - 60 min), temperature ($30-70^{\circ}C$), solvent (from non-polar to polar), and ratio of solvnet / sample (10 : 1 - 40 : 1) using ultrasonic extractor. Swertiamarin in SJ extracts was quantified by high performance liquid chromatography - Phtodiode array detector (HPLC-PDA) using C18 column and the analytical procedure was validated by evaluation of specificity, range, linearity, accuracy (recovery), precision (intra- and inter day variability), limit of detection (LOD), and limit of quantification (LOQ). Results : An efficient extraction condition for swertiamarin in SJ was optimized using sonicator extraction (temperature $40^{\circ}C$, solvent 20% methanol, solvent / sample (20 : 1), and time 10 min. Analytical procedure was optimized by HPLC-PDA using isocratic solvent system of acetonitrile and water (9 : 91), and the method was validated in regard to linearity (correlation coefficient, $R^2$ > 0.9999), range ($50-1000{\mu}g/mL$), intra- and inter-precision (RSD < 5.0 %), and recovery (99 -103 %). LOD and LOQ were 0.051 and $0.155{\mu}g/mL$, respectively. Conclusion : An optimized method of extraction for swertiamarin in SJ was established through conditions of diverse extraction and the validation result indicated that the method is suited for the determination of swertiamarin in SJ.

Keywords

References

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