Genes and Genomics

The Genetics Society of Korea (한국유전학회)
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Genomic cloning and promoter analysis of the ${\beta}$-actin gene from Korean rose bitterling (Rhodeus uyekii)

  • Kong, Hee Jeong (Biotechnology Research Division, National Fisheries Research and Development Institute) ;
  • Kim, Ju Lan (Biotechnology Research Division, National Fisheries Research and Development Institute) ;
  • Kim, Woo-Jin (Biotechnology Research Division, National Fisheries Research and Development Institute) ;
  • Kim, Hyung Soo (Biotechnology Research Division, National Fisheries Research and Development Institute) ;
  • Yeo, Sang-Yeob (Division of Applied Chemistry and Biotechnology, Hanbat National University) ;
  • Park, Jung Youn (Biotechnology Research Division, National Fisheries Research and Development Institute) ;
  • An, Cheul Min (Biotechnology Research Division, National Fisheries Research and Development Institute)
  • Received : 2014.07.24
  • Accepted : 2014.09.01
  • Published : 2014.12.01
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Abstract

Korean rose bitterling (Rhodeus uyekii), which belongs to the Acheilognathinae subfamily of the Cyprinidae family, has been proposed as a candidate for the development of ornamental fish in Korea. To develop a promoter capable of driving constitutive transgene expression, we identified and characterized the ${\beta}$-actin gene of R. uyekii (Ru-actb). The genomic organization of Ru-actb, which contains six exons, including an unexpressed first exon, is conserved with vertebrate ${\beta}$-actin genes. The Ru-actb gene has several regulatory elements, including a typical CAAT box, an evolutionarily conserved CArG motif, and a TATA box, in the proximal promoter region, and an additional CArG motif in the first intron. The regulatory region of Ru-actb covering from -4,474 bp to the start codon was able to drive the expression of red fluorescent protein in zebrafish and Korean rose bitterling. Ru-actb mRNA was ubiquitously detected in all tissues; highly expressed in brain, kidney, stomach, and intestine; and weakly expressed in eye, gill, fin, hepatopancreas, spleen, muscle, testis, and ovary. These results indicate that the regulatory region of Ru-actb may be used to develop a useful promoter for transgene expression in fish.

Keywords

Acknowledgement

Supported by : NFRDI

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