Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지
DOI QR코드

DOI QR Code

Investigation of Antimicrobial Activity and Stability of Orixa japonica Thunb. Leaf Extract

상산나무 잎 추출물의 항균활성 및 안정성 조사

  • Choe, Su-Bin (Department of Food Science and Technology, Seoul National University of Science and Technology) ;
  • Kang, Sung-Tae (Department of Food Science and Technology, Seoul National University of Science and Technology)
  • 최수빈 (서울과학기술대학교 식품공학과) ;
  • 강성태 (서울과학기술대학교 식품공학과)
  • Received : 2013.09.30
  • Accepted : 2013.11.26
  • Published : 2014.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

The antimicrobial activity of Orixa japonica Thunb. leaf extract towards 13 microorganism strains was evaluated. Both methanol (MEex) and 70% ethanol extracts showed antimicrobial activity towards Streptococcus mutans, Bacillus cereus, Staphylococcus aureus, and Pseudomonas aeruginosa. MEex showed a higher antimicrobial activity than the 70% ethanol extract. In addition, the dichloromethane fraction (DCMfr) of the MEex also had an antimicrobial effect against the microorganisms examined. The minimum inhibitory concentrations (MICs) towards S. mutans, B. cereus, S. aureus, and P. aeruginosa were 49.22, 24.61, 49.22, and 49.22 mg/mL, respectively. In contrast, the MICs of the DCMfr tpwards S. mutans, B. cereus, S. aureus, and P. aeruginosa were 3.31, 0.21, 1.7, and 1.7 mg/mL, respectively. The MEex antimicrobial activity was not affected by a 3 h exposures to pH in the range of 3-11 or by temperatures were maintained between $80^{\circ}C-100^{\circ}C$ for 6 h. However, the MEex antimicrobial activity decreased at a heat treatment of $121^{\circ}C$ 1 h.

상산나무 잎의 항미생물 활성을 알아보기 위하여 상산나무 잎을 메탄올, 70% 에탄올, $65^{\circ}C$ 열수로 추출하였고 각각의 추출물에 대하여 13종의 미생물에 대하여 항미생물 활성을 paper disc diffusion법으로 측정하였다. 그 결과 4개 균주 S. mutans, B. cereus, S. aureus, P. aeruginosa가 메탄올 추출물, 70% 에탄올 추출물에서 clear zone을 형성하여 추출물에 의하여 생육이 저해되는 것을 확인할 수 있었고 methanol 추출물이 70% ethanol 추출물에 비하여 항균활성이 크게 나타났다. 이에 methanol 추출물을 극성이 다른 여러 용매로 분획추출하고 항균활성을 측정한 결과 dichloromethane 분획물에서 항균활성을 나타냈으며 다른 분획물인 n-hexane, ethyl acetate, butanol, water 분획물에서는 항균활성이 나타나지 않았다. 가장 활성이 크게 나타났던 메탄올 추출물의 항균활성을 보인 4개의 균에 대하여 최소저해농도를 측정한 결과 S. mutans, S. aureus, P. aeruginosa는 추출물의 농도가 49.22 mg/mL에서 항균활성이 나타났으며 B. cereus의 경우 24.61 mg/mL에서 항균활성을 나타내었으며 S. mutans, B. cereus, S. aureus, P. aeruginosa에 대한 dichloromethane 분획물의 최소저해농도는 각각 3.31, 0.21, 1.7, 1.7 mg/mL으로 나타났다. 메탄올 추출물의 pH에 대한 안정성을 알아보기 위하여 pH 3, 7, 11로 조절하여 1-3시간 동안 처리한 결과 항균활성에는 영향을 주지 않았고 열에 대한 안정성을 확인하기 위하여 80, $100^{\circ}C$에서 1-6시간 동안 처리한 결과 대조군과 차이가 없었고 $120^{\circ}C$ 처리한 경우 항균활성이 약간 저하되었으나 항균활성이 완전히 실활 되지 않은 것으로 보아 상산나무 잎 methanol 추출물은 열과 pH에 비교적 안정하여 식품의 가공 처리에도 적합한 천연 식품첨가물로 유용하게 이용될 수 있을 것으로 사료된다.

Keywords

References

  1. Yoon SY, Lee SY, Kim KBWR, Song EJ, Kim SJ, Lee SJ, Lee CJ, Ahn DH. Antimicrobial activity of the solvent extract from different parts of Orostachys japonicus. J. Korean Soc. Food Sci. Nutr. 38: 14-18 (2009) https://doi.org/10.3746/jkfn.2009.38.1.014
  2. Lee IE, Cho SH. Antimicrobial effect of Aristolochia contorta Bge. extract on the growth of pathogenic and putrefactive microorganisms. J. Korean Soc. Food Sci. Nutr. 29: 1107-1111 (2000)
  3. Ha YM, Lee BB, Bae HJ, Je KM, Kim SR, Choi JS, Choi IS. Anti-microbial activity of grapefruit seed extract and processed sulfur solution against human skin pathogens. J. Life Science 19: 94-100 (2009) https://doi.org/10.5352/JLS.2009.19.1.094
  4. Kim HY. Antimicrobial activity and characteristics of Amblytropis pauciflora Kitagawa extract. J. Korean Soc. Food Sci. Nutr. 27: 993-999 (1998)
  5. Park JS, Shim CJ, Jung JH, Lee GH, Sung CK, Oh MJ. Antimicrobial activity of Ulmi cortex extracts. J. Korean Soc. Food Sci. Nutr. 28: 1022-1028 (1999)
  6. Lee HO, Han DM, Baek SH. Isolation and identification of anticariotic compound from Sophora flavescens Ait. Kor. J. Microbiol Biotechnol. 30: 420-424 (2002)
  7. Jang GH, Ahn BY, Oh SH, Choi DS, Kwon YJ. Anticariogenic effects of Coptis chinensis Franch extract. Korean J. Food Sci. Technol. 32: 1396-1402 (2000)
  8. Bae KH, Ji JM, Park KL. The antimicrobial component from Cinnamomi cortex against a cariogenic bacterium Streptococcus mutans OMZ 176. Arch. Pharm. Res. 15: 239-241 (1992) https://doi.org/10.1007/BF02974062
  9. Bae KH, Kim BH, Myung PK, Chung KS, Baek JH. The isolation and evaluation of bioactive components from crude drugs against a cariogenic bacterium, Streptococcus mutans OMZ 176. Yakhak Hoeji 34: 277-281 (1990)
  10. Katsura H, Tsukiyama RI, Suzuki A, Kobayashi M. In vitro antimicrobial activities of bakuchiol against oral microorganisms. Antimicrob. Agents Ch. 45: 3009-3013 (2001) https://doi.org/10.1128/AAC.45.11.3009-3013.2001
  11. Hwang JK, Chung JY, Baek NI, Park JH. Isopanduratin A from Kaempferia pandurata as an active antibacterial agent against cariogenic Streptococcus mutans. Int. J. Antimicrob. Ag. 23: 377-381 (2004) https://doi.org/10.1016/j.ijantimicag.2003.08.011
  12. Park KM, You JS, Lee HY, Baek NI, Hwang JK. Kuwanon G: antibacterial agents from the root bark of Morus alba against oral pathogens. J. Ethnopharmacol. 84: 181-185 (2003) https://doi.org/10.1016/S0378-8741(02)00318-5
  13. Sohn HY, Kum EJ, Kwon YS, Kwon GS, Jin I, Kwon HY, Kwon CS, Son KH. Screening of anticandidosis agent from medicinal and wild plants. Kor. J. Life Sci. 13: 604-617 (2003) https://doi.org/10.5352/JLS.2003.13.5.604
  14. Lee YN. New Flora of Korea. Vol. 1. Kyohaksa, Seoul, Korea. pp. 401-490 (2006)
  15. Hong WS. The use of herbs and herbal ingredients. Vol. 2. Ilwolseogak, Seoul, Korea. p. 317 (1999)
  16. Jang CS, Fu FY, Huang KC, WANG CY. Pharmacology of Ch'ang Shan (Dichroa febrifuga), a chinese antimalarial herb. Nature 161: 400-401 (1948)
  17. Koepfli JB, Mead JF and Brockman JA. An alkaloid with high antimalarial activity from Dichroa febrifuga. J. Am. Chem. Soc. 69: 1837 (1947) https://doi.org/10.1021/ja01199a513
  18. Coatney GR, Cooper WC, Culwell WB, White WC and Imboden CA. Studies in human malaria. XXV. Trial of febrifugine, an alkaloid obtained from Dichroa febrifuga Lour., against the Chesson strain of Plasmodium vivax. J. Natl. Malar. Soc. 9: 183-186 (1950)
  19. Katsura Ito. Overwintering survival and postdiapause fecundity in a population of the Kanzawa spider mite Tetranychus kanzawai (Acari: Tetranychidae) on Orixa japonica Thunb.(Rutaceae). Exp. Appl. Acarol. 53: 51-56 (2011) https://doi.org/10.1007/s10493-010-9383-7
  20. Katsura Ito. Effect of host plants on diapause induction in immature and adult Tetranychus kanzawai (Acari: Tetranychidae). Exp. Appl. Acarol. 52: 11-17 (2010) https://doi.org/10.1007/s10493-010-9342-3
  21. Ono H, Kuwahara Y, Nishida R. Hydroxybenzoic acid derivatives in a nonhost rutaceous plant, Orixa japonica Thunb., deter both oviposition and larval feeding in a rutaceae-feeding swallowtail butterfly, Papilio xuthus L. J. Chem. Ecol. 30: 287-301 (2004) https://doi.org/10.1023/B:JOEC.0000017978.73061.a0
  22. Kim KW, BaekJ K, Jang YW, Kum EJ, Kwon YS, Kim HJ, Sohn HY. Screening of antibacterial agent against Streptococcus mutans from natural and medicinal plants. J. Life Science 15: 715-725 (2005) https://doi.org/10.5352/JLS.2005.15.5.715
  23. Badk DH. Screening of the natural plant extracts for the antimicrobial activity on dental pathogens. Kor. J. Microbiol. 43: 227-231 (2007)
  24. Lee SY, Song EJ, Kim KBWR, Yoon SY, Kim SJ, Lee SJ, Hong YK, Lim SM, Ahn DH. Antimicrobial activity of ethanol extract from Sargassum thunbergii. J. Korean Soc. Food Sci. Nutr. 38: 502-508 (2009) https://doi.org/10.3746/jkfn.2009.38.4.502
  25. Yoon SY, Lee SY, Kim KBWR, Song EJ, Lee SJ, Lee CJ, Park NB, Jung JY, Kwak JH, Nam KW, Ahn DH. Antimicrobial activity of the Sargassum fulvellum ethanol extract and the effect of temperature and pH on their activity. Korean J. Food Sci. Technol. 42: 155-159 (2010)

Cited by

  1. Evaluation of Antioxidant and Antimicrobial Activities of Solvent Extracts from Coriolus versicolor vol.44, pp.12, 2015, https://doi.org/10.3746/jkfn.2015.44.12.1793
  2. Antimicrobial Effects on Food-Borne Pathogens and the Antioxidant Activity of Torreya Nucifera Extract vol.26, pp.4, 2015, https://doi.org/10.7856/kjcls.2015.26.4.697