Journal of Korean Medical Science

Korean Acad Medical Sciences (대한의학회)
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Significance of Serum Antibody Test for Toxocariasis in Healthy Healthcare Examinees with Eosinophilia in Seoul and Gyeongsangnam-do, Korea

  • Kim, Hong Seok (Department of Parasitology and Tropical Medicine, Institute of Endemic Diseases, Seoul National University College of Medicine) ;
  • Jin, Yan (Department of Parasitology and Tropical Medicine, Institute of Endemic Diseases, Seoul National University College of Medicine) ;
  • Choi, Min-Ho (Department of Parasitology and Tropical Medicine, Institute of Endemic Diseases, Seoul National University College of Medicine) ;
  • Kim, Jae-Hwan (Department of Environmental and Tropical Medicine, Konkuk University School of Medicine) ;
  • Lee, Young Ha (Korea Association of Health Promotion) ;
  • Yoon, Cheong Ha (Korea Association of Health Promotion) ;
  • Hwang, Eui-Hyuk (Korea Association of Health Promotion) ;
  • Kang, Hun (Korea Association of Health Promotion) ;
  • Ahn, Sang-Yong (Korea Association of Health Promotion) ;
  • Kim, Gi Jin (Korea Association of Health Promotion) ;
  • Hong, Sung-Tae (Department of Parasitology and Tropical Medicine, Institute of Endemic Diseases, Seoul National University College of Medicine)
  • Received : 2014.08.21
  • Accepted : 2014.09.29
  • Published : 2014.12.01
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Abstract

There have been numerous reports on the relationship between eosinophilia and toxocariasis. The present study investigated seropositive rates of toxocariasis among healthy people with or without eosinophilia in urban and rural areas, and assessed risk factors for positive antibody test. A total of 610 healthy people, who visited health check-up (Medicheck$^{(R)}$, Korea Association of Health Promotion), 310 from Seoul and 300 from Gyeongsangnam-do, were subjected for this study. Their serum samples were tested by ELISA with the crude antigen of Toxocara canis larvae. Cross-reactions with other tissue invading helminth antigens were also investigated. Total antibody positive rate of toxocariasis was 8.7% of the 610 subjects. When the subjects were grouped into 3 by their eosinophil counts, the antibody positive rates significantly differed by the groups; 5.9% (18/306) in the group<$350/{\mu}L$, 10.0% (11/110) in the group $350-500/{\mu}L$, and 12.4% (24/194) in the group>$500/{\mu}L$ (P=0.028). A total of 22 serum samples cross-reacted with other tissue-invading helminth antigens. A questionnaire analysis recognized drinking alcohol and smoking as significant risk factors of toxocariasis. In conclusion, toxocariasis antibody positive rate is correlated with eosinophil counts. It is recommended that healthy subjects with eosinophilia by routine health examination and risk factors undergo Toxocara serology by multiantigen ELISA to investigate etiology.

Keywords

References

  1. Rubinsky-Elefant G, Hirata CE, Yamamoto JH, Ferreira MU. Human toxocariasis: diagnosis, worldwide seroprevalences and clinical expression of the systemic and ocular forms. Ann Trop Med Parasitol 2010; 104: 3-23. https://doi.org/10.1179/136485910X12607012373957
  2. Ashwath ML, Robinson DR, Katner HP. A presumptive case of toxocariasis associated with eosinophilic pleural effusion: case report and literature review. Am J Trop Med Hyg 2004; 71: 764.
  3. Jung JK, Jung JT, Lee CH, Kim EY, Kwon JG, Kim BS. A case of hepatic abscess caused by Toxocara. Korean J Hepatol 2007; 13: 409-13. https://doi.org/10.3350/kjhep.2007.13.3.409
  4. Park SP, Park I, Park HY, Lee SU, Huh S, Magnaval JF. Five cases of ocular toxocariasis confirmed by serology. Korean J Parasitol 2000; 38: 267-73. https://doi.org/10.3347/kjp.2000.38.4.267
  5. Lee JY, Kim BJ, Lee SP, Jeung YJ, Oh MJ, Park MS, Paeng JW, Lee BJ, Choi DC. Toxocariasis might be an important cause of atopic myelitis in Korea. J Korean Med Sci 2009; 24: 1024-30. https://doi.org/10.3346/jkms.2009.24.6.1024
  6. Cho SY, Kang SY, Ryang YS. Helminthes infections in the small intestine of stray dogs in Ejungbu City, Kyunggi Do, Korea. Korean J Parasitol 1981; 19: 55-9. https://doi.org/10.3347/kjp.1981.19.1.55
  7. Huh S, Sohn WM, Chai JY. Intestinal parasites of cats purchased in Seoul. Korean J Parasitol 1993; 31: 371-3. https://doi.org/10.3347/kjp.1993.31.4.371
  8. Kim YH, Huh S. Prevalence of Toxocara canis, Toxascaris leonina and dirofilaria immitis in dogs in Chuncheon, Korea (2004). Korean J Parasitol 2005; 43: 65-7. https://doi.org/10.3347/kjp.2005.43.2.65
  9. Park HY, Lee SU, Huh S, Kong Y, Magnaval JF. A seroepidemiological survey for toxocariasis in apparently healthy residents in Gangwon-do, Korea. Korean J Parasitol 2002; 40: 113-7. https://doi.org/10.3347/kjp.2002.40.3.113
  10. Kwon NH, Oh MJ, Lee SP, Lee BJ, Choi DC. The clinical impact of toxocariasis in patients with unknown eosinophilia. Korean J Asthma Allergy Clin Immunol 2005; 25: 299-304.
  11. Seo M, Yoon SC. A seroepidemiological survey of toxocariasis among eosinophilia patients in Chungcheongnam-do. Korean J Parasitol 2012; 50: 249-51. https://doi.org/10.3347/kjp.2012.50.3.249
  12. Kim YH, Huh S, Chung YB. Seroprevalence of toxocariasis among healthy people with eosinophilia. Korean J Parasitol 2008; 46: 29-32. https://doi.org/10.3347/kjp.2008.46.1.29
  13. Won KY, Kruszon-Moran D, Schantz PM, Jones JL. National seroprevalence and risk factors for zoonotic Toxocara spp. infection. Am J Trop Med Hyg 2008; 79: 552-7.
  14. Companion Animal Parasite Council. Parasite prevalence maps. Available at http://www.capcvet.org/parasite-prevalence-maps/ [accessed on 26 September 2014].
  15. Woodhall DM, Eberhard ML, Parise ME. Neglected parasitic infections in the United States: toxocariasis. Am J Trop Med Hyg 2014; 90: 810-3. https://doi.org/10.4269/ajtmh.13-0725
  16. Jin Y, Shen C, Huh S, Sohn WM, Choi MH, Hong ST. Serodiagnosis of toxocariasis by ELISA using crude antigen of Toxocara canis larvae. Korean J Parasitol 2013; 51: 433-9. https://doi.org/10.3347/kjp.2013.51.4.433
  17. Jacquier P, Gottstein B, Stingelin Y, Eckert J. Immunodiagnosis of toxocarosis in humans: evaluation of a new enzyme-linked immunosorbent assay kit. J Clin Microbiol 1991; 29: 1831-5.
  18. Mohamad S, Azmi NC, Noordin R. Development and evaluation of a sensitive and specific assay for diagnosis of human toxocariasis by use of three recombinant antigens (TES-26, TES-30USM, and TES-120). J Clin Microbiol 2009; 47: 1712-7. https://doi.org/10.1128/JCM.00001-09
  19. Magnaval JF, Fabre R, Maurieres P, Charlet JP, de Larrard B. Application of the western blotting procedure for the immunodiagnosis of human toxocariasis. Parasitol Res 1991; 77: 697-702. https://doi.org/10.1007/BF00928685
  20. Yamasaki H, Araki K, Lim PK, Zasmy N, Mak JW, Taib R, Aoki T. Development of a highly specific recombinant Toxocara canis second-stage larva excretory-secretory antigen for immunodiagnosis of human toxocariasis. J Clin Microbiol 2000; 38: 1409-13.
  21. Romasanta A, Romero JL, Arias M, Sanchez-Andrade R, Lopez C, Suarez JL, Diaz P, Diez-Banos P, Morrondo P, Paz-Silva A. Diagnosis of parasitic zoonoses by immunoenzymatic assays--analysis of cross-reactivity among the excretory/secretory antigens of Fasciola hepatica, Toxocara canis, and Ascaris suum. Immunol Invest 2003; 32: 131-42. https://doi.org/10.1081/IMM-120022974
  22. Ishida MM, Rubinsky-Elefant G, Ferreira AW, Hoshino-Shimizu S, Vaz AJ. Helminth antigens (Taenia solium, Taenia crassiceps, Toxocara canis, Schistosoma mansoni and Echinococcus granulosus) and cross-reactivities in human infections and immunized animals. Acta Trop 2003; 89: 73-84. https://doi.org/10.1016/j.actatropica.2003.09.005
  23. Choi D, Lim JH, Choi DC, Paik SW, Kim SH, Huh S. Toxocariasis and ingestion of raw cow liver in patients with eosinophilia. Korean J Parasitol 2008; 46: 139-43. https://doi.org/10.3347/kjp.2008.46.3.139
  24. Ryu BH, Park JS, Jung YJ, Kang SK, Lee SH, Choi SJ. Clinical and serological findings in patients with toxocariasis in the Pohang region: the features of toxocariasis in Pohang. Korean J Med 2013; 84: 203-10. https://doi.org/10.3904/kjm.2013.84.2.203
  25. Kang YR, Kim SA, Jeon K, Koh WJ, Suh GY, Chung MP, Kim H, Kwon OJ, Kang ES, Um SW. Toxocariasis as a cause of new pulmonary infiltrates. Int J Tuberc Lung Dis 2013; 17: 412-7. https://doi.org/10.5588/ijtld.12.0273
  26. Zukiewicz M, Kaczmarski M, Topczewska M, Sidor K, Tomaszewska BM. Epidemiological and clinical picture of parasitic infections in the group of children and adolescents from north-east region of Poland. Wiad Parazytol 2011; 57: 179-87.
  27. Schoenardie ER, Scaini CJ, Brod CS, Pepe MS, Villela MM, McBride AJ, Borsuk S, Berne ME. Seroprevalence of Toxocara infection in children from southern Brazil. J Parasitol 2013; 99: 537-9. https://doi.org/10.1645/GE-3182
  28. Lim JH. Foodborne eosinophilia due to visceral larva migrans: a disease abandoned. J Korean Med Sci 2012; 27: 1-2.
  29. Kim YJ, Moon CH, Chang JH. Toxocariasis of the optic disc. J Neuroophthalmol 2013; 33: 151-2. https://doi.org/10.1097/WNO.0b013e3182893ff9
  30. Yoon YS, Lee CH, Kang YA, Kwon SY, Yoon HI, Lee JH, Lee CT. Impact of toxocariasis in patients with unexplained patchy pulmonary infiltrate in Korea. J Korean Med Sci 2009; 24: 40-5. https://doi.org/10.3346/jkms.2009.24.1.40
  31. Ahn SJ, Woo SJ, Jin Y, Chang YS, Kim TW, Ahn J, Heo JW, Yu HG, Chung H, Park KH, et al. Clinical features and course of ocular toxocariasis in adults. PLoS Negl Trop Dis 2014; 8: e2938. https://doi.org/10.1371/journal.pntd.0002938
  32. Manini MP, Marchioro AA, Colli CM, Nishi L, Falavigna-Guilherme AL. Association between contamination of public squares and seropositivity for Toxocara spp. in children. Vet Parasitol 2012; 188: 48-52. https://doi.org/10.1016/j.vetpar.2012.03.011
  33. Noh Y, Hong ST, Yun JY, Park HK, Oh JH, Kim YE, Jeon BS. Meningitis by Toxocara canis after ingestion of raw ostrich liver. J Korean Med Sci 2012; 27: 1105-8. https://doi.org/10.3346/jkms.2012.27.9.1105
  34. Korea Center for Disease Control and Prevention. The 8th National Survey for Intestinal Parasites in Korea. Osong: Korea Center for Disease Control and Prevention, 2013.

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