Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
권호 표지
DOI QR코드

DOI QR Code

Effective DNA extraction method to improve detection of Mycobacterium avium subsp. paratuberculosis in bovine feces

  • Park, Hong-Tae (Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University) ;
  • Shin, Min-Kyoung (Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University) ;
  • Sung, Kyung Yong (Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University) ;
  • Park, Hyun-Eui (Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University) ;
  • Cho, Yong-Il (Department of Animal Resources Development, National Institute of Animal Science, Rural Development Administration) ;
  • Yoo, Han Sang (Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University)
  • Received : 2013.12.13
  • Accepted : 2014.03.14
  • Published : 2014.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

Paratuberculosis caused by Mycobacterium avium subsp. paratuberculosis (MAP) has extended latent periods of infection. Due to this property, difficulties in the detection of fecal shedder have been raised. A newly designed method for DNA extraction from fecal specimens, mGITC/SC was evaluated in terms of diagnostic efficiency. The detection limit of IS900 real-time PCR was about 50 MAP (1.5 cfu) in 250 mg of feces (6 cfu per g). Also, this DNA extraction method was faster and cheaper than that using commercial kit or other methods. Consequently, the mGITC/SC is an economical DNA extraction method that could be a useful tool for detecting MAP from fecal specimens.

Keywords

References

  1. Amaro A, Duarte E, Amado A, Ferronha H, Botelho A. Comparison of three DNA extraction methods for Mycobacterium bovis, Mycobacterium tuberculosis and Mycobacterium avium subsp. avium. Lett Appl Microbiol 2008, 47, 8-11. https://doi.org/10.1111/j.1472-765X.2008.02372.x
  2. Boom R, Sol CJA, Salimans MMM, Jansen CL, Wertheimvan Dillen PME, van der Noordaa J. Rapid and simple method for purification of nucleic acids. J Clin Microbiol 1990, 28, 495-503.
  3. Bull TJ, Hermon-Taylor J, Pavlik I, El-Zaatari F, Tizard M. Characterization of IS900 loci in Mycobacterium avium subsp. paratuberculosis and development of multiplex PCR typing. Microbiology 2000, 146, 2185-2197. https://doi.org/10.1099/00221287-146-9-2185
  4. Collins MT. Diagnosis of paratuberculosis. Vet Clin North Am Food Anim Pract 1996, 12, 357-371. https://doi.org/10.1016/S0749-0720(15)30411-4
  5. Irenge LM, Walravens K, Govaerts M, Godfroid J, Rosseels V, Huygen K, Gala J-L. Development and validation of a triplex real-time PCR for rapid detection and specific identification of M. avium sub sp. paratuberculosis in faecal samples. Vet Microbiol 2009, 136, 166-172. https://doi.org/10.1016/j.vetmic.2008.09.087
  6. Leite FL, Stokes KD, Robbe-Austerman S, Stabel JR. Comparison of fecal DNA extraction kits for the detection of Mycobacterium avium subsp. paratuberculosis by polymerase chain reaction. J Vet Diagn Invest 2013, 25, 27-34. https://doi.org/10.1177/1040638712466395
  7. Logar K, Kopinc R, Bandelj P, Staric J, Lapanje A, Ocepek M. Evaluation of combined high-efficiency DNA extraction and real-time PCR for detection of Mycobacterium avium subsp. paratuberculosis in subclinically infected dairy cattle: comparison with faecal culture, milk real-time PCR and milk ELISA. BMC Vet Res 2012, 8, 49. https://doi.org/10.1186/1746-6148-8-49
  8. Monteiro L, Bonnemaison D, Vekris A, Petry KG, Bonnet J, Vidal R, Cabrita J, Megraud F. Complex polysaccharides as PCR inhibitors in feces: Helicobacter pylori model. J Clin Microbiol 1997, 35, 995-998.
  9. Odumeru J, Gao A, Chen S, Raymond M, Mutharia L. Use of the bead beater for preparation of Mycobacterium paratuberculosis template DNA in milk. Can J Vet Res 2001, 65, 201-205.
  10. Ott SL, Wells SJ, Wagner BA. Herd-level economic losses associated with Johne's disease on US dairy operations. Prev Vet Med 1999, 40, 179-192. https://doi.org/10.1016/S0167-5877(99)00037-9
  11. Ravva SV, Stanker LH. Real-time quantitative PCR detection of Mycobacterium avium subsp. paratuberculosis and differentiation from other mycobacteria using SYBR Green and TaqMan assays. J Microbiol Methods 2005, 63, 305-317. https://doi.org/10.1016/j.mimet.2005.04.004
  12. Thornton CG, Passen S. Inhibition of PCR amplification by phytic acid, and treatment of bovine fecal specimens with phytase to reduce inhibition. J Microbiol Methods 2004, 59, 43-52. https://doi.org/10.1016/j.mimet.2004.06.001
  13. Whitlock RH, Wells SJ, Sweeney RW, Van Tiem J. ELISA and fecal culture for paratuberculosis (Johne's disease): sensitivity and specificity of each method. Vet Microbiol 2000, 77, 387-398. https://doi.org/10.1016/S0378-1135(00)00324-2
  14. Whittington RJ, Marshall DJ, Nicholls PJ, Marsh IB, Reddacliff LA. Survival and dormancy of Mycobacterium avium subsp. paratuberculosis in the environment. Appl Environ Microbiol 2004, 70, 2989-3004. https://doi.org/10.1128/AEM.70.5.2989-3004.2004
  15. Zhang MZ, Zhang S. An efficient DNA extraction method for polymerase chain reaction-based detection of Mycobacterium avium subspecies paratuberculosis in bovine fecal samples. J Vet Diagn Invest 2011, 23, 41-48. https://doi.org/10.1177/104063871102300106

Cited by

  1. PCR-based detection of Mycobacterium avium subsp. paratuberculosis infection in cattle in South Korea using fecal samples vol.78, pp.9, 2016, https://doi.org/10.1292/jvms.15-0271
  2. Gene expression profiles of putative biomarker candidates inMycobacterium aviumsubsp.paratuberculosis-infected cattle vol.74, pp.4, 2016, https://doi.org/10.1093/femspd/ftw022
  3. Potential biomarkers as an indicator of vertical transmission of Johne's disease in a Korean native cattle farm vol.18, pp.S1, 2017, https://doi.org/10.4142/jvs.2017.18.S1.343
  4. PCR Inhibition of a Quantitative PCR for Detection of Mycobacterium avium Subspecies Paratuberculosis DNA in Feces: Diagnostic Implications and Potential Solutions vol.08, 2017, https://doi.org/10.3389/fmicb.2017.00115
  5. An ISMap 02 -like insertion sequence in Mycobacterium spp. interferes with specific detection of Mycobacterium avium subsp. paratuberculosis vol.216, 2018, https://doi.org/10.1016/j.vetmic.2018.01.013
  6. PCR-REA, MIRU-VNTR, and MLSSR genotyping vol.19, pp.5, 2018, https://doi.org/10.4142/jvs.2018.19.5.627
  7. -Infected Cattle vol.48, pp.4, 2018, https://doi.org/10.4167/jbv.2018.48.4.130
  8. Multiplex Quantitative Real-time Polymerase Chain Reaction Assay for Rapid Detection of Mycobacterium avium subsp. paratuberculosis in Fecal Samples vol.32, pp.3, 2014, https://doi.org/10.17555/jvc.2015.06.32.3.219
  9. 16S and 23S rRNA Gene Mutation Independent Multidrug Resistance of Non-Tuberculous Mycobacteria Isolated from South Korean Soil vol.8, pp.8, 2020, https://doi.org/10.3390/microorganisms8081114
  10. Comparative analysis of serological tests and fecal detection in the diagnosis of Mycobacterium avium subspecies paratuberculosis infection vol.60, pp.3, 2014, https://doi.org/10.14405/kjvr.2020.60.3.117
  11. Genomic diversity of Mycobacterium avium subsp. paratuberculosis: pangenomic approach for highlighting unique genomic features with newly constructed complete genomes vol.52, pp.1, 2014, https://doi.org/10.1186/s13567-021-00905-1