Applied Chemistry for Engineering (공업화학)

The Korean Society of Industrial and Engineering Chemistry (한국공업화학회)
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Study on Antibacterial Activity of Ag Nanometal-deposited TiO2 Prepared by Sonochemical Reduction Method

초음파환원법에 의해 제조된 Ag-TiO2의 항균 활성도 고찰

  • Jung, Hye Yeon (Department of Chemical and Biochemical Engineering, Gachon University) ;
  • Lee, Sang-Wha (Department of Chemical and Biochemical Engineering, Gachon University)
  • 정혜연 (가천대학교 화공생명공학과) ;
  • 이상화 (가천대학교 화공생명공학과)
  • Received : 2013.11.02
  • Accepted : 2013.12.14
  • Published : 2014.02.10
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Abstract

In this work, Ag-$TiO_2$ nanocomposites were prepared via the sonochemical deposition of Ag nanometals on $TiO_2$ nanoparticles. The size of deposited Ag nanometals was ranged in 1~3 nm and the number of Ag nanometals deposited on $TiO_2$ increased in proportion to the dosage amounts of Ag precursors. As-prepared Ag-$TiO_2$ was loaded on the sterilized agar plate together with an aliquot volume of diluted E-coli, followed by 30 min irradiation of the solar simulated light ($600{\sim}1800{\mu}w/cm^2$). Finally, the agar plate was incubated for 24 h at $37^{\circ}C$ and the number of survived colonies were counted. It was experimentally confirmed that Ag-$TiO_2$ exhibited the higher antimicrobial activity than that of pure $TiO_2$, based on measuring the colony number of control sample. The survived colony numbers on the agar plate decreased with the increase of dosage amounts of Ag-$TiO_2$ and the irradiated intensity of solar simulated light for 30 min before incubating. The increase of Ag nanometal doposition induced the progressive enhancement of antimicrobial activity, but rather reduced the photocatalytic activity of Ag-$TiO_2$ probably due to the excessive presence of Ag nanometals on $TiO_2$ matrix.

본 연구에서는 초음파환원법을 이용하여 이산화티탄($TiO_2$) 미립자 표면에 은나노메탈이 도핑된 Ag-$TiO_2$ 나노복합체를 제조하였다. $TiO_2$ 표면에 생성되는 은나노금속은 약 1~3 nm의 사이즈분포를 나타내었고, 환원반응시 첨가되는 $AgNO_3$의 양이 증가할수록 $TiO_2$ 표면에 형성되는 은나노금속의 개수가 증가하였다. 이렇게 얻어진 일정량의 Ag-$TiO_2$ 나노복합체를 대장균(E-coli)과 함께 고체멸균배지에 도말하여 태양광모사 제논램프로부터 30 min간 $600{\sim}1800{\mu}w/cm^2$의 빛을 조사한 후 $37^{\circ}C$에서 24 h 배양한 후 생존한 콜로니의 개수를 측정하였다. 실험 결과 대조군대비 순수한 $TiO_2$를 첨가했을 때보다 Ag-$TiO_2$를 첨가 시 항균활성도가 더 높게 나타났다. 또한 Ag-$TiO_2$ 주입양이 증가할수록 콜로니의 개수가 감소하였고, 초기 30 min간 조사한 빛의 세기가 증가할수록 Ag-$TiO_2$의 항균효과가 증가하였다. 또한 은나노금속의 도핑양이 증가할수록 광촉매 효율은 감소하였지만 항균효과는 지속적으로 증가하는 경향을 나타내었다.

Keywords

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