Journal of the East Asian Society of Dietary Life (동아시아식생활학회지)

The East Asian Society of Dietary Life (동아시아식생활학회)
권호 표지

Comparison of Antioxidant and ${\alpha}$-Glucosidase Inhibition Activities among Water Extracts and Sugar Immersion Extracts of Green Pepper, Purslane and Shiitake

청고추, 쇠비름, 표고버섯의 물 추출물 및 당침액의 항산화 활성 및 ${\alpha}$-Glucosidase Inhibition 활성 비교

  • Lee, Sung Mee (Dept. of Bio-Health Technology, Kangwon National University) ;
  • Kang, Yun Hwan (Well-being Bioproducts RIC, Kangwon National University) ;
  • Kim, Dae Jung (Well-being Bioproducts RIC, Kangwon National University) ;
  • Kim, Kyoung Kon (Dept. of Bio-Health Technology, Kangwon National University) ;
  • Lim, Jun Gu (Well-being Bioproducts RIC, Kangwon National University) ;
  • Kim, Tae Woo (Well-being Bioproducts RIC, Kangwon National University) ;
  • Choe, Myeon (Dept. of Bio-Health Technology, Kangwon National University)
  • 이성미 (강원대학교 생명건강공학과) ;
  • 강윤환 (강원대학교 강원웰빙특산물산업화지역혁신센터) ;
  • 김대중 (강원대학교 강원웰빙특산물산업화지역혁신센터) ;
  • 김경곤 (강원대학교 생명건강공학과) ;
  • 임준구 (강원대학교 강원웰빙특산물산업화지역혁신센터) ;
  • 김태우 (강원대학교 강원웰빙특산물산업화지역혁신센터) ;
  • 최면 (강원대학교 생명건강공학과)
  • Received : 2013.12.12
  • Accepted : 2014.02.28
  • Published : 2014.02.28
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Abstract

This study was conducted in order to compare the biological activities of water extracts and sugar immersion extracts of green pepper (Capsicum annuum L.), purslane (Portulaca oleracea L.) and shiitake (Lentinula edodes (Berk.) Pegler) by measuring total polyphenol and flavonoid contents, antioxidant activities and inhibitory effects on ${\alpha}$-amylase and ${\alpha}$-glucosidase. The contents of total polyphenols and flavonoids were higher in water extracts than in sugar immersion extracts. The anti-oxidative activities of water and sugar immersion extracts were measured using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging activity assay and reducing power assay. All extracts scavenged radicals in a concentration-dependent manner, and water extracts showed stronger radical scavenging activity and reducing power than sugar immersion extract. However, they all exhibited lower activities than ascorbic acid. Compared to the anti-diabetic drug acarbose, which was used as a positive control, the two types of extracts exhibited low ${\alpha}$-glucosidase inhibitory activities, although the activity of sugar immersion extracts were 2-fold higher than that of water extracts. ${\alpha}$-Amylase inhibitory action was not observed for any of the extracts. Finally, by cytotoxicity test, we confirmed that sugar immersion extracts were safer than water extracts. These results indicate that water extracts and sugar immersion extracts of green pepper, purslane and shiitake have different advantages in terms of their antioxidant and anti-diabetic effects, respectively.

본 연구에서는 최근 건강 관리를 위해 다양하게 상용되고 있는 당침액의 오 남용을 막기 위해 과학적으로 물 추출물과 당침액의 특성을 규명하고자 하였다. 그 결과, 일반 물 추출물과 비교하였을 때 당침액의 총 폴리페놀 및 플라보노이드 함량이 크게 감소하는 것을 확인하였으며, 그에 따라 항산화 활성 또한 감소한 것을 확인할 수 있었다. 이는 물 추출물과 비교하여 당침액의 건조 분말에는 설탕 또는 그 분해물들이 많은 부분을 차지하기 때문이라 판단된다. 그러나 혈당강하 효능의 target 효소로 연구되고 있는 ${\alpha}$-amylase 및 ${\alpha}$-glucosidase의 활성을 측정한 결과, 청고추, 쇠비름, 표고버섯의 물 추출물과 당침액은 ${\alpha}$-amylase 억제 활성을 나타내지 않았지만, ${\alpha}$-glucosidase 억제 활성은 의약품인 acarbose에는 미치지 못하였으나, 물 추출물에 비해 당침액이 농도 의존적으로 높은 억제 활성을 나타내었다. 또한 세포 독성을 HepG2 cell에서 확인한 결과, 물 추출물과 비교하여 당침액의 독성이 약 1/10 감소한 것을 확인할 수 있었다. 이와 같은 결과는 민간에서의 믿음처럼 당침액이 물 추출물과 비교하였을 때 모든 부분에서 우수한 기능을 가진다고 할 수 없으며, 활용할 용도에 따라 소재를 물 추출하거나 당침액을 제조하는 등의 가공 과정을 거치는 것이 목표한 기능을 효과적으로 확보하는 방법임을 의미한다. 따라서 민간에서 맹목적으로 믿는 당침액의 효능에 대한 과학적인 연구 접근법은 당침액의 적절한 사용 목적과 방법을 설정하는 기초 자료로 활용이 가능할 것이다.

Keywords

References

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