The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Mycelial Growth and Extracellular Enzyme Activities of Wood-decaying Mushroom Strains on Solid Media

고체배지 상에서 목재부후성 버섯 균주들의 균사생장 특성과 세포외효소 활성

  • Jeon, Sung-Min (Division of Wood Chemistry & Microbiology, Korea Forest Research Institute) ;
  • Ka, Kang-Hyeon (Division of Wood Chemistry & Microbiology, Korea Forest Research Institute)
  • 전성민 (국립산림과학원 화학미생물과) ;
  • 가강현 (국립산림과학원 화학미생물과)
  • Received : 2014.03.09
  • Accepted : 2014.03.19
  • Published : 2014.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

The mycelial growths of wood-decaying mushroom strains collected from Korean forests were investigated on solid media under different culture media and temperatures. Most of strains showed the higher mycelial growth on potato dextrose agar (PDA) than malt extract agar (MEA) or sabouraud dextrose agar (SDA) plates. Except for a few strains, they grew well on PDA at $25^{\circ}C$ and showed a poor growth at low temperature ($10^{\circ}C$) than high temperature ($30^{\circ}C$). All strains showed the carboxymethylcellulase (CM-cellulase) and laccase activities on solid media containing the specific substrates for two different enzymes.

우리나라 산림에서 수집한 목재부후성 버섯 균주들에 대한 배지 및 온도별 균사생장 특성을 고체평판배지 상에서 조사하였다. 대부분의 균주들은 malt extract agar (MEA)나 sabouraud dextrose agar (SDA)보다는 PDA (potato dextrose agar) 배지에서 균사 생장력이 더 높았다. 몇몇 균주들을 제외하고, 균주들의 대부분은 PDA 배지 상에서 배양 시 $25^{\circ}C$에서 가장 잘 생장하였으며, 고온($30^{\circ}C$)보다는 저온($10^{\circ}C$)에서 균사 생장이 저조하였다. 2종류의 서로 다른 세포외효소 활성에 각각 특이적으로 반응할 수 있는 기질이 함유된 고체평판배지 상에서 모든 시험균은 carboxymethyl cellulase 활성과 laccase 활성을 나타냈다.

Keywords

References

  1. Ka KH, Jeon SM, Ryoo R, Ryu SH, Kim MG, Bak WC, Park JW, Koo CD, Eom AH. Management of genetic resources of forest microorganisms. Research report: Korea Forest Research Institute; 2011.
  2. Jang TW, Jun SC, Ahn TS, Kim KJ. Production of lignin degrading enzymes and decolorization of various dye compounds by wood-rot fungi. Kor J Mycol 2006;42:34-39.
  3. Kasana RC, Salwan R, Dhar H, Dutt S, Gulati A. A rapid and easy method for the detection of microbial cellulases on agar plates using gram's iodine. Curr Microbiol 2008;57:503-507. https://doi.org/10.1007/s00284-008-9276-8
  4. Crowe JD, Olsson S. Induction of laccase activity in Rhizoctonia solani by antagonistic Pseudomonas fluorecens strains and a range of chemical treatments. Appl Environ Microbiol 2001;7:2088-2094.
  5. Kalmis E, Yasa I, Kalyoncu F, Pazarba B, Kocyigit A. Ligninolytic enzyme activities in mycelium of some wild and commercial mushrooms. Afr J Biotechnol 2008;7:4314-4320.
  6. Niku-Paavola ML, Raaska L, Itavaara M. Detection of white rot fungi by a non-toxic stain. Mycol Res 1990;94:27-31. https://doi.org/10.1016/S0953-7562(09)81260-4
  7. Jeon SM, Kim MS, Ka KH. Effects of medium, temperature and mycelia growth and cellulase activity of ectomycorrhizal fungi from Korean forests. Kor J Mycol 2012;40:191-203. https://doi.org/10.4489/KJM.2012.40.4.191
  8. Hankin L, Anagnostakis SL. Solid media containing carboxymethylcellulose to detect Cx cellulase activity of micro-organisms. J Gen Microbiol 1977;98:109-115. https://doi.org/10.1099/00221287-98-1-109
  9. Zhang YHP, Himmel ME, Mielenz JR. Outlook for cellulase improvement: Screening and selection. Biotechnol Adv 2006; 24:452-481. https://doi.org/10.1016/j.biotechadv.2006.03.003
  10. Giardina P, Faraco V, Pezzella C, Piscitelli A, Vanhulle S, Sannia G. Laccases: a never-ending story. Cell Mol Life Sci 2010; 67:369-385. https://doi.org/10.1007/s00018-009-0169-1
  11. Baldrian P. Fungal laccases-occurrence and properties. FEMS Microbiol Rev 2006;30:215-242. https://doi.org/10.1111/j.1574-4976.2005.00010.x
  12. Das N, Sengupta S, Mukherjee M. Importance of Laccase in vegetative growth of Pleurotus florida. Appl Environ Microbiol 1997;63:4120-4122.

Cited by

  1. Characteristics of Mycelial Growth and Enzyme Activities of Mattirolomyces terfezioides Collected from Robinia pseudoacacia Forest in Korea vol.43, pp.3, 2015, https://doi.org/10.4489/KJM.2015.43.3.165
  2. Cultural Characteristics of Korean Ectomycorrhizal Fungi vol.43, pp.1, 2015, https://doi.org/10.4489/KJM.2015.43.1.1
  3. Effects of Preservation Period at Low Temperature on the Mycelial Growth and the Lignocellulolytic Enzyme Activities of Basidiomycetes vol.42, pp.4, 2014, https://doi.org/10.4489/KJM.2014.42.4.322