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Preparation of Cheonggukjang added onion (Allium cepa L.) and its antioxidative activity

양파(Allium cepa L.) 첨가 청국장의 제조 및 항산화능 평가

  • Lee, Minji (Department of Food Science and Technology, and Functional Food Research Center, Chonnam National University) ;
  • Lee, Yu Geon (Department of Food Science and Technology, and Functional Food Research Center, Chonnam National University) ;
  • Cho, Jung-Il (Department of Food Nutrient and Culinary, Chosun College of Science and Technology) ;
  • Na, Kwang-Chool (Department of Food Nutrient and Culinary, Chosun College of Science and Technology) ;
  • Hwang, Eom Ji (Bioenergy Crop Research Center, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Mi Seung (Muan-gun Agritechnology Center) ;
  • Moon, Jae-Hak (Department of Food Science and Technology, and Functional Food Research Center, Chonnam National University)
  • 이민지 (전남대학교 식품공학과 및 기능성식품연구센터) ;
  • 이유건 (전남대학교 식품공학과 및 기능성식품연구센터) ;
  • 조정일 (조선이공대학교 식품영양조리과학과) ;
  • 나광출 (조선이공대학교 식품영양조리과학과) ;
  • 황엄지 (농촌진흥청 국립식량과학원 바이오에너지작물센터) ;
  • 김미승 (무안군 농업기술센터) ;
  • 문제학 (전남대학교 식품공학과 및 기능성식품연구센터)
  • Received : 2013.10.22
  • Accepted : 2013.12.12
  • Published : 2014.02.28

Abstract

Yellow and black soybean Cheonggukjangs (YBSC) prepared with an addition of onion (Allium cepa L.) in different contents (0, 5, 10, 20, and 30%, w/w) were manufactured, and the sensory evaluation was carried out. The optimum addition ratio of onion was determined to be 20%. Total phenolic and flavonoid contents of YBSC prepared with an addition of onion were higher than those of the YBSC prepared without an onion. In addition, YBSC prepared with an addition of onion showed higher 1,1-diphenyl-2-picrylhydrazyl radical-scavenging activities than those prepared without an onion. Furthermore, rat blood plasma 1 hour after oral administration of YBSC prepared with an onion was more effective in suppressing the accumulation of cholesteryl ester hydroperoxide than those prepared without onion and control. These results indicated that the consumption of the YBSC prepared with onion may contribute to the antioxidant defense in vivo.

양파를 첨가한 청국장 제조 가능성을 평가하고, 제조된 시료들을 대상으로 항산화능을 비교하였다. 먼저 양파의 적정 첨가량을 판단하기 위해 노랑콩과 검정콩 각각의 중량을 기준으로 0, 5, 10, 20, 30%가 되도록 양파를 첨가하여 청국장을 제조한 후 관능평가를 실시하였다. 그 결과, 노랑콩과 검정콩 청국장 모두 20% 양파 첨가 청국장이 높은 기호도를 나타냈다. 그래서 20%의 양파를 첨가하여 제조한 청국장을 대상으로 특정성분의 함량 및 항산화능을 비교 평가하였다. 그 결과, 양파를 첨가하여 제조한 청국장이 첨가하지 않은 청국장보다 유의하게 더 높은 총 페놀성 및 flavonoid 함량을 나타냈다. 그리고 DPPH radical-scavenging 활성평가에 있어서도 양파를 첨가하여 제조한 청국장이 첨가하지 않고 제조한 청국장보다 더 높은 radical-scavenging 활성을 나타냈다. 또 쥐 혈장 산화에 따른 CE-OOH 생성 억제능 평가에 있어서도 양파 첨가 청국장 추출물을 투여한 쥐혈장이 양파를 첨가하지 않은 청국장 추출물을 투여한 쥐 혈장에 비해 더 높은 산화 억제능을 나타냈다. 이상의 결과들은 양파에 다량 함유된 quercetin을 포함한 flavonoid들과 발효과정 중 생성되는 다양한 페놀성 화합물들에 의한 효과라 판단된다. 본 연구결과가 국산 양파의 가공시장확대 및 청국장 소비의 저변확대를 위한 기초자료로 활용되길 기대한다.

Keywords

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